Neuron-specific enolase as a marker of the severity and outcome of hypoxic ischemic encephalopathy

Çeltik, Coşkun; Acunaş, Betül; Öner, Naci; Pala, Özer

doi:10.1016/j.braindev.2003.12.007

Cited by 100 publications

(76 citation statements)

References 16 publications

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“…The incidence of apnea in the 5-cm group was significantly higher than that in the 7.5-cm and 10-cm groups, and recovery time of spontaneous breathing was much longer. Studies suggested that the serum NSE concentration is a sensitive biomarker for the degree of brain injury [17]. In this study, the NSE concentration increased early postexplosion in the 7.5-cm group, reaching a peak at 1 d, which may imply the early leakage of intracellular NSE into the blood.…”

Section: Discussionmentioning

confidence: 87%

Development of a rat model for studying blast-induced traumatic brain injury

Cheng

et al. 2010

Journal of the Neurological Sciences

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Section: Discussionmentioning

confidence: 87%

Development of a rat model for studying blast-induced traumatic brain injury

Cheng

et al. 2010

Journal of the Neurological Sciences

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“…Scale bar is 30 μm Figure 6 Using immunofluorescent technique, the expressions of NSE (6A) and MMP-9 (6B) can be observed in the hippocampus in ischemic 1.5 h/reperfusion 24 h. And the coexpression of NSE and MMP-9 proteins is shown in the same cell with double immunofluorescence staining (6C). Scale bar is 30 μm in the early diagnosis, severity, and prognosis of brain injury (Sulemanji et al 2007;Celtik et al 2004). Our study showed that NSE-positive cells temporarily increased in the control group at ischemia 0.5 h and reached the peak at ischemia 1 h. This illustrates that ischemia-reperfusion can induce the expression of NSE and its transient increase may be spontaneous protection mechanisms against brain injury at early stage of cerebral ischemia (<1 h) so as to enhance the anti-anoxia ability of nerve cells.…”

Section: Discussionmentioning

confidence: 99%

Effects of Neuregulin on Expression of MMP-9 and NSE in Brain of Ischemia/Reperfusion Rat

Rui

et al. 2008

J Mol Neurosci

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The aim is to investigate the effects of neuregulin-1beta (NRG-1beta) on expression of matrix metalloproteinase-9 (MMP-9) and neuron-specific enolase (NSE) in brain tissue in rats following cerebral ischemia/reperfusion. One hundred and fifty adult healthy male Wistar rats were used in the present study. Ten of them were randomized into a sham-operation group (n = 10) and the rest suffered surgery operation of middle cerebral artery occlusion/reperfusion with intraluminal monofilament suture from the left external-internal carotid artery. As a result, 100 rats of successful models were randomly divided into a control group (n = 50) and a treatment group (n = 50). Rats in the treatment group were injected 1.5% NRG-1beta at a dosage of 0.3 microg/kg from the stump of the left external carotid artery into the internal carotid artery. The expressions of MMP-9 and NSE proteins were determined by immunohistochemical, immunofluorescent double labeling, and Western blot assay. Ischemia/reperfusion induced morphological changes of brain tissue, including neurocyte shrinkage, chromatin condensation, nuclei fragment, and gliacyte and endothelial cell swelling. NRG-1beta obviously reduced and delayed the cerebral damage. With the duration of ischemia, the expression of MMP-9 gradually increased in the control group. NRG-1beta decreased the level of MMP-9 compared with that in the control group (P < 0.01). NSE immunoreaction transiently elevated at the early stage of cerebral ischemia insult, and then gradually decreased in the control group. The administration of NRG-1beta significantly increased the level of NSE, and thus delayed the time and the degree of neuron damage. There were statistical differences in contrast to the control group (P < 0.01). There was no relationship between the expressions of the two proteins. MMP-9 might aim at various target cells at different stages and contribute to the inflammatory reaction after cerebral ischemia-reperfusion insult. NRG-1beta inhibits the activation of MMP-9 and development of inflammation, enhances the activity of NSE, improves the microenvironment of neuron survivals, and delays the phase of irreversible neuron necrosis. Therefore, NRG-1beta may play a neuroprotective role in cerebral ischemia/reperfusion.

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“…Serupa dengan laporan penelitian sebelumnya, Thornberg dkk 9 melaporkan rerata kadar NSE CSS pada EHI derajat 1, 2, dan 3 berturut-turut 17,2; 34,5; dan 185 µg/L . Garcia-Alix dkk, 10 melakukan penelitian kohort prospektif terhadap 69 bayi asfiksia yang mengalami 12 pada bayi dengan sindroma aspirasi mekoneum yang mengalami komplikasi EHI, melaporkan kadar NSE serum pada EHI derajat 1, 2, dan 3 berturut-turut (65,3±32,4), (64,6±32,9), dan (115±60,9) µg/L. Hasil tersebut berbeda dengan penelitian kami, mungkin disebabkan perbedaan sampel.…”

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Kadar Neuron-Specific Enolase Serum dan Derajat Ensefalopati Hipoksik Iskemik pada Asfiksia Neonatorum

Wijata

Wilar²,

Warouw³

et al. 2016

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Latar belakang. Ensefalopati hipoksik iskemik (EHI) merupakan komplikasi asfiksia neonatorum yang mengenai sistem saraf pusat berupa disfungsi serebrovaskular dan oksigenasi, gangguan neurotransmiter, degenerasi sel-sel neuron, edema serebral yang berakhir pada kematian sel. Penelitian sebelumnya mendapatkan peningkatan kadar neuron specific enolase (NSE) cairan serebrospinal pada bayi dengan EHI. Masih belum banyak penelitian mengenai kadar NSE serum pada bayi dengan EHI. Tujuan. Mengetahui hubungan kadar NSE serum dengan derajat EHI pada asfiksia neonatorum. Metode. Penelitian analitik observasional pendekatan potong lintang dilakukan dari bulan Oktober 2015 sampai Februari 2016. Sampel adalah bayi asfiksia yang mengalami EHI, diambil secara konsekutif. Analisis statistik diuji dengan korelasi Spearman. Orang tua atau wali diminta menandatangani informed consent. Hasil. Kami mendapatkan 39 bayi asfiksia yang mengalami EHI, terdiri atas 20 bayi laki-laki dan 19 perempuan. Terdapat hubungan bermakna antara kadar NSE serum dengan derajat EHI pada asfiksia neonatorum (koefisien korelasi r =0,893 nilai p<0,001). Kesimpulan. Terdapat korelasi positif antara kadar NSE dengan derajat EHI pada asfiksia neonatorum. Sari Pediatri 2016;18(1):1-5Kata kunci: neuron-specific enolase, ensefalopati hipoksik iskemik, asfiksia neonatorum Serum Neuron-Specific Enolase and Hypoxic Ischemic Encephalopathy Degree in Neonatal AsphyxiaAgus Wijata, Rocky Wilar, Sarah M. Warouw, Johnny Rompis Background. Hypoxic ischemic encephalopathy (HIE) is a poor complication of neonatal asphyxia. Previous studies showed elevated levels of cerebrospinal fluid neuron specific enolase (NSE) in infant with HIE. There are very few studies have been conducted about serum NSE level in infant with HIE. Objective. To determine the relationship of serum NSE levels with HIE degree in neonatal asphyxia Method. We conducted an observational study with cross-sectional approach from October 2015 until February 2016. Samples were asphyxiated newborn with HIE based on Sarnat and Sarnat modification criteria. Samples were taken consecutively. The relationship between serum NSE levels with HIE degree were tested with Spearman correlation analysis. Informed consent was attained from parents or guardians. This study was approved by ethical comittee Prof. Dr. R. D. Kandou Hospital Manado. Results. We found 39 asphyxiated newborns with HIE, consisted of 20 boys and 19 girls. There was a significant correlation between serum NSE levels with HIE degree in neonatal asphyxia (correlation coefficient r s =0.893 with p<0.001). Conclusion. The higher levels of serum NSE, the greater degree of HIE in asphyxia neonatorum. Sari Pediatri 2016;18(1):1-5

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Neuron-specific enolase as a marker of the severity and outcome of hypoxic ischemic encephalopathy

Cited by 100 publications

References 16 publications

Development of a rat model for studying blast-induced traumatic brain injury

Development of a rat model for studying blast-induced traumatic brain injury

Effects of Neuregulin on Expression of MMP-9 and NSE in Brain of Ischemia/Reperfusion Rat

Kadar Neuron-Specific Enolase Serum dan Derajat Ensefalopati Hipoksik Iskemik pada Asfiksia Neonatorum

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