Neuronal development and migration in zebrafish hindbrain explants

Bingham, Stephanie; Toussaint, Gesulla; Chandrasekhar, Anand

doi:10.1016/j.jneumeth.2005.05.002

Cited by 9 publications

(9 citation statements)

References 38 publications

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“…We favor a model in which the importance of the second cell is not in having pioneer-like capacity, but rather in being the closest FBMN to the pioneer, serving to link the pioneer to follower FBMNs. Much previous work examining FBMN migration has focused on the PCP pathway; several labs have shown that multiple components of the PCP pathway are crucial for FBMN migration both in zebrafish and in mouse Jessen et al, 2002;Carreira-Barbosa et al, 2003;Bingham et al, 2005;Wada et al, 2005;Wada et al, 2006;Rohrschneider et al, 2007;Vivancos et al, 2009;Qu et al, 2010;Walsh et al, 2011;Glasco et al, 2012). Our analysis of pioneer neurons and the MLF supports a model in which FBMN migration requires additional inputs beyond PCP signaling.…”

Section: Research Articlesupporting

confidence: 69%

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Axon tracts guide zebrafish facial branchiomotor neuron migration through the hindbrain

Wanner

Prince

2013

Development

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SUMMARYAppropriate localization of neurons within the brain is a crucial component of the establishment of neural circuitry. In the zebrafish hindbrain, the facial branchiomotor neurons (FBMNs) undergo a chain-like tangential migration from their birthplace in rhombomere (r) 4 to their final destination in r6/r7. Here, we report that ablation of either the cell body or the trailing axon of the leading FBMN, or 'pioneer' neuron, blocks the migration of follower FBMNs into r5. This demonstrates that the pioneer neuron and its axon are crucial to the early migration of FBMNs. Later migration from r5 to r6 is not dependent on pioneer neurons but on the medial longitudinal fasciculus (MLF), a bundle of axons lying ventral to the FBMNs. We find that MLF axons enter r5 only after the pioneer neuron has led several followers into this region; the MLF is then contacted by projections from the FBMNs. The interactions between FBMNs and the MLF are important for migration from r5 to r6, as blocking MLF axons from entering the hindbrain can stall FBMN migration in r5. Finally, we have found that the adhesion molecule Cdh2 (N-cadherin) is important for interactions between the MLF and FBMNs, as well as for interactions between the trailing axon of the pioneer neuron and follower FBMNs. Interestingly, migration of pioneer neurons is independent of both the MLF and Cdh2, suggesting pioneer migration relies on independent cues.

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Section: Research Articlesupporting

confidence: 69%

“…Tg(zCREST1:membRFP) embryos were placed into explant media (Bingham et al, 2005) containing L15 media (Gibco), 1 M glucose (Sigma) and pen/strep (Sigma) in a depression slide and oriented in 2% methylcellulose. The brain was severed at r2 using a set of Lumsden BioScissors (Oban Precision Instruments) at 14 hpf.…”

Section: Mlf Surgical Blockmentioning

confidence: 99%

Axon tracts guide zebrafish facial branchiomotor neuron migration through the hindbrain

Wanner

Prince

2013

Development

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“…4J), showing that migrating motor neurons also extend axons. A timelapse study using hindbrain explants (Bingham et al, 2005) (see Materials and methods) confirmed that the motor neurons were always located near the pial surface of the hindbrain during migration (Fig. 5A, see also Movie S1 in the supplementary material).…”

Section: Research Articlementioning

confidence: 64%

“…The procedures for time-lapse observations were essentially the same as described (Langenberg et al, 2003;Bingham et al, 2005) with modifications (H.T. and H.O., unpublished).…”

Section: Time-lapse Observationsmentioning

confidence: 99%

Frizzled3a and Celsr2 function in the neuroepithelium to regulate migration of facial motor neurons in the developing zebrafish hindbrain

et al. 2006

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Migration of neurons from their birthplace to their final target area is a crucial step in brain development. Here, we show that expression of the off-limits/frizzled3a (olt/fz3a) and off-road/celsr2 (ord/celsr2) genes in neuroepithelial cells maintains the facial (nVII) motor neurons near the pial surface during their caudal migration in the zebrafish hindbrain. In the absence of olt/fz3a expression in the neuroepithelium, nVII motor neurons extended aberrant radial processes towards the ventricular surface and mismigrated radially to the dorsomedial part of the hindbrain. Our findings reveal a novel role for these genes, distinctive from their already known functions, in the regulation of the planar cell polarity (i.e. preventing integration of differentiated neurons into the neuroepithelial layer). This contrasts markedly with their reported role in reintegration of neuroepithelial daughter cells into the neuroepithelial layer after cell division.

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“…The zebrafish, Danio rerio, has been extensively used as a model for studying gene expression in developing vertebrates [19,20]. A transient high expression of HSP 70 arising in zebrafish early larvae at 80-84 h post fertilization (hpf) was repeatedly observed in our previous studies [21,22].…”

Section: Introductionmentioning

confidence: 81%

A spontaneous high expression of heat shock cognate 70 (HSC 70) in zebrafish Danio rerio larvae arising from tissue-specific translation of preexisting mRNA

et al. 2010

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A transient high expression of heat shock protein 70 (HSP 70) in zebrafish Danio rerio at 84 h post fertilization (hpf) suspected to arise from translational control of heat shock cognate 70 (hsc 70) mRNA has been detected in our previous studies. In this report, we examined the patterns of development-regulated hsc 70 mRNA translation in zebrafish tissues and the possible mechanism of translational control. HSP 70 spontaneously expressed in 84 hpf zebrafish contained HSC 70 (pI 5.5) and stressinduced HSP 70 (pI 5.7) at a 10:1 ratio based on 2-D Western blotting. Whole mount in situ hybridization showed very similar patterns of hsc 70 transcription in the head regions and swim bladders of 60-84 hpf zebrafish, while hsp 70 transcripts appeared only in the lens. As in situ immunohistochemistry detected an intense level of HSC 70 synthesis in the swim bladders of 84 hpf zebrafish, but not in those of 60 hpf zebrafish, HSC 70 produced by up-regulated hsc 70 mRNA translation should be critical to certain stages of swim bladder development. RT-PCR-poly(A) tail (RT-PCR-PAT) assay excluded the possible involvement of poly(A) tail elongation in regulating hsc 70 mRNA translation. Unlike zebrafish hsp 70 mRNA, hsc 70 mRNA lacking a long 5 0 untranslated region (5 0 -UTR) that might provide an internal ribosome entry site displayed a weak translatability in rabbit reticulocyte lysates, suggesting that hsc 70 mRNA translation in zebrafish tissues is controlled by the availability of cap-binding proteins.

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Neuronal development and migration in zebrafish hindbrain explants

Cited by 9 publications

References 38 publications

Axon tracts guide zebrafish facial branchiomotor neuron migration through the hindbrain

Axon tracts guide zebrafish facial branchiomotor neuron migration through the hindbrain

Frizzled3a and Celsr2 function in the neuroepithelium to regulate migration of facial motor neurons in the developing zebrafish hindbrain

A spontaneous high expression of heat shock cognate 70 (HSC 70) in zebrafish Danio rerio larvae arising from tissue-specific translation of preexisting mRNA

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