Neuronal nitric oxide synthase in the olfactory system, forebrain, pituitary and retina of the adult teleost Clarias batrachus

Gaikwad, Archana; Biju, K.C.; Barsagade, Vikas G.; Bhute, Yogesh; Subhedar, Nishikant K.

doi:10.1016/j.jchemneu.2008.12.006

Cited by 14 publications

(44 citation statements)

References 87 publications

(88 reference statements)

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“…Comparatively, intensely NOS-ir cells are present in the paraventricular area of cladistians [López et al, 2016] and their projections could be followed towards the neurohypophysis. Similar nitrergic groups with hypothalamo-hypophysial projections have been reported in the electric ray [Pérez et al, 1995], in the teleost Clarias batrachus [Gaikwad et al, 2009], and in anuran amphibians [Allaerts et al, 1997;Prasada Rao et al, 1997], although some amphibian species may lack nitrergic cells in the paraventricular region [Pinelli et al, 2014]. In mammals, the NADPH-d-reactive cells of the neurosecretory hypothalamic nuclei coexpress vasopressin/oxytocin [Arévalo et al, 1992;Miyagawa et al, 1994;Sánchez et al, 1994], where the NO probably regulates the release of these neurohormones [Vanhatalo and Soinila, 1995], whereas this colocalization is absent in the hypothalamus of birds [Sánchez et al, 1996].…”

Section: Forebrainsupporting

confidence: 55%

“…Compared to other vertebrates, the presence of nitrergic cells in the suprachiasmatic nucleus is a generally conserved feature ( Table 2) that has been reported in most species of teleosts and reptiles, although with some exceptions [Jiang and Terashima, 1996;Gaikwad et al, 2009], in birds and some species of amphibians [Lázár and Losonczy, 1999;González et al, Huynh and Boyd, 2007] and mammals [Amir et al, 1995;Wang and Morris, 1996;Agostino et al, 2004], but not in other species of these groups [Mizukawa et al, 1989;Vincent and Kimura, 1992;Egberongbe et al, 1994;Brüning and Mayer, 1996;González et al, 1996;Pinelli et al, 2014]. The colocalization of NOS and TH in the suprachiasmatic neurons of gars seems to be a specific feature of this group of fishes, since it has not been observed +, presence of NOS-ir cells; -, absence of NOS-ir cells; +/-, presence of NOS-ir cells in some species of the group; ?, no data available; PPT, pedunculopontine tegmentum.…”

Section: Forebrainmentioning

confidence: 91%

“…In the pallium, gars possess scattered and faintly NOS-ir cells separated from the ventricle in the lateral zone of the dorsal telencephalic area (Dl), largely comparable to the nitrergic population observed in the pallial field P3 of cladistians [López et al, 2016]. In teleosts, a common pattern is seen in the pallium with nitrergic cells in the Dl and in dorsal and medial zones of the dorsal telencephalic area, although in some species the total lack of nitrergic elements in the pallium has been reported [Arévalo et al, 1995;Gaikwad et al, 2009]. Because of the eversion process, the pallial Dl of gars, as the pallial P3 of cladistians, has been interpreted as the homolog of the medial pallium of the noneverted telencephalon of tetrapods [Bruce and Braford, 2008;Braford, 2009;Nieuwenhuys, 2011], and the presence of similar scattered nitrergic cells described in the medial pallium of lungfishes [González and Northcutt, 2009] and amphibians Muñoz et al, 1996; supports this homology.…”

Section: Forebrainmentioning

confidence: 94%

“…References: agnathans: Schober et al, 1994a;Kyriakatos et al, 2009;chondrichthyes: Shiells and Falk, 1992;Pérez et al, 1995;Álvarez-Otero et al, 1996;Funakoshi et al, 1997;Ferrando et al, 2012;Pose-Mendez et al, 2014;cladistians: López et al, 2016;chondrosteans: Pushchina and Obukhov, 2012;teleosts: Holmqvist et al, 1994teleosts: Holmqvist et al, , 2000Ostholm et al, 1994;Arévalo et al, 1995;Brüning et al, 1995;Anken and Rahmann, 1996;Cioni et al, 1997;Pottek et al, 1997;Funakoshi et al, 1999;Baby et al, 2000;Oyan et al, 2000;Haamedi and Djamgoz, 2002;Bordieri et al, 2003Bordieri et al, , 2005Singru et al, 2003;Ando et al, 2004;Bordieri and Cioni, 2004;Pushchina and Diuĭzen, 2004;Pushchina, 2007;Pushchina et al, 2007;Giráldez-Pérez et al, 2008Gaikwad et al, 2009;Pushchina and Obukhov, 2010;Ganz et al, 2012;lungfishes: Schober et al, 1994b;Northcutt, 2009;amphibians: Nöll et al, 1994;Crowe et al, 1995;Kurenni et al, 1995;Brüning and Mayer, 1996;González et al, 1996González et al, , 2002b…”

Section: Forebrainmentioning

confidence: 99%

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Pattern of Nitrergic Neuronal System Organization in the Brain of Two Holostean Fishes (Actinopterygii: Ginglymodi)

et al. 2017

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The study of the nitrergic system, formed by the networks of neurons containing the enzyme nitric oxide synthase (NOS), has been extremely useful in unraveling neuroanatomical features of the organization of the central nervous system of vertebrates. Thus, data are available for representatives of most vertebrate classes and, in particular, several studies have detailed the organization of this system in teleosts. In contrast, no information is available regarding this neurotransmission system in the brains of holosteans, an early diverged and poorly understood group of actinopterygian fishes, currently considered a sister group of teleosts that contains only 8 species. The present study provides the first detailed information on the distribution of nitrergic cell bodies and fibers in 2 holostean species of the genus Lepisosteus, the spotted gar L. oculatus and the Florida gar L. platyrhincus. NOS immunohistochemistry and the NADPH diaphorase (NADPH-d) histochemical reaction were used, and both techniques yielded identical results, with the exception of the primary olfactory and terminal nerve fibers, which only labeled for NADPH-d exclusively in L. oculatus. Double immunohistochemistry was conducted for the simultaneous detection of NOS with tyrosine hydroxylase, choline acetyltransferase, calbindin, calretinin, and serotonin to accurately establish the localization of the nitrergic neurons and fibers in the brain of holosteans, the neuroanatomy of which has been mostly neglected, and to assess possible interactions between these neuroactive substances. Distinct groups of nitrergic cells were located in subpallial areas, the basal hypothalamus, posterior tubercle, optic tectum and mesencephalic tegmentum, reticular formation, solitary tract nucleus, spinal cord, and amacrine cells in the retina. In addition, low numbers of nitrergic cells were observed in the pallium, suprachiasmatic nucleus, prethalamic and thalamic areas, torus lateralis and torus semicircularis, cerebellar and laterodorsal tegmental nuclei, and the ventral octavolateral area. Comparison of these results with those from other classes of vertebrates, and including a segmental analysis to correlate cell populations, reveals that the pattern of the nitrergic system in holosteans is very close to that in ancestral actinopterygian fishes and highlights conserved and derived traits.

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Section: Forebrainsupporting

confidence: 55%

Section: Forebrainmentioning

confidence: 91%

Section: Forebrainmentioning

confidence: 94%

Section: Forebrainmentioning

confidence: 99%

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Pattern of Nitrergic Neuronal System Organization in the Brain of Two Holostean Fishes (Actinopterygii: Ginglymodi)

et al. 2017

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“…In addition, ciliated olfactory neurons of the normal epithelium were immunoreactive to nNOS. Previous reports have also described nNOS immunoreactivity in receptor neurons from olfactory epithelium in Oreochromis mossambicus (Peters) (Singru et al 2003) and Clarias batrachus (L.) (Gaikwad et al 2009). Thus, immunoreactivity of the goldfish ONB cells with this antibody is plausible.…”

mentioning

confidence: 78%

First description of an olfactory neuroblastoma in goldfish Carassius auratus: a case report

Vigliano

Marcaccini²,

Sarradell³

et al. 2011

Dis. Aquat. Org.

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An external pinkish growing mass that emerged from the right nostril of an adult goldfish Carassius auratus L. was evaluated by means of light microscopy and immunohistochemistry. The neoplasm presented a well-developed fibrovascular stroma associated with solid cell nests and a large number of Flexner-Wintersteiner rosettes. Myelinated fibres were observed around them. Neoplastic cells showed a prominent degree of nuclear atypia and low mitotic activity. The latter was in agreement with the low reactivity of tumour cells to anti-proliferating cell nuclear antigen antibody. Immunohistochemistry also revealed anti-neuronal nitric oxide synthase, anti-S100 protein, antineuropeptide Y, and anti-cytokeratin immunoreactivity in tumour cells as well as in normal olfactory epithelium of goldfish control sections. Histopathological and immunohistochemical findings strongly suggest a diagnosis of an olfactory neuroblastoma (ONB). To our knowledge this is the first description of ONB in goldfish. KEY WORDS: Tumour · Olfactory neuroblastoma · Goldfish · Carassius auratus · Histopathology · Immunohistochemistry Resale or republication not permitted without written consent of the publisherDis Aquat Org 96: [61][62][63][64][65][66][67][68] 2011 MATERIALS AND METHODSAn adult goldfish Carassius auratus L. was referred to a private veterinary clinic located in Rosario City, Argentina. The specimen showed an external pinkish growing mass emerging from the right nostril. The roughly shaped mass of approximately 0.5 cm in diameter was excised, fixed in 10% v/v buffered formaldehyde solution and submitted to the Diagnostic Pathology Service of the School of Veterinary Sciences-National University of Rosario, Argentina. The sample was dehydrated in a graded series of ethanol and embedded in paraffin wax. Sections of 3 to 5 µm in thickness were stained using haematoxylin and eosin, combined periodic acid-Schiff (PAS)/Alcian blue, the Grimelius technique and Tolivia staining.In order to define the origin of the presumptive tumour, an immunohistochemical study was carried out. Unless otherwise stated, all incubations were performed at room temperature in a humid chamber, and all washing procedures consisted of 3 successive 5 min immersions in 0.1 M phosphate-buffered saline (PBS). Endogenous peroxidase activity was blocked by incubation in Peroxidase Blocking Reagent (Dako) for 30 min, and after a rinse in PBS, antigens were exposed by heating under pressure in different buffer solutions or by incubating the samples with proteolytic enzymes depending on the primary antibody employed ( Table 1). The sections were subsequently washed again with PBS, treated with 3% skimmed milk powder for 15 min to block non-specific antibody binding, incubated with the primary antibody as indicated in Table 1, washed with PBS, and incubated for 30 min with anti-mouse or anti-rabbit EnVision+ System Labelled Polymer-HRP (DakoCytomation). After further rinsing, the sections were finally developed using 3, 3-diaminobenzidine tetrahydrochloride...

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Organization of the nitrergic neuronal system in the primitive bony fishesPolypterus senegalusandErpetoichthys calabaricus(Actinopterygii: Cladistia)

López

Lozano

Morona

et al. 2015

J of Comparative Neurology

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Cladistians are a group of basal actinopterygian fishes that constitute a good model for studying primitive brain features, most likely present in the ancestral bony fishes. The analysis of the nitrergic neurons (with the enzyme nitric oxide synthase; NOS) has helped in understanding important aspects of brain organization in all vertebrates studied. We investigated the nitrergic system of two cladistian species by means of specific antibodies against NOS and NADPH-diaphorase (NADPH-d) histochemistry, which, with the exception of the primary olfactory and terminal nerve fibers, labeled only for NADPH-d, yielded identical results. Double immunohistochemistry was conducted for simultaneous detection of NOS with tyrosine hydroxylase, choline acetyltransferase, calbindin, calretinin, and serotonin, to establish accurately the localization of the nitrergic neurons and fibers and to assess possible interactions between these neuroactive substances. The pattern of distribution in both species showed only subtle differences in the density of labeled cells. Distinct groups of NOS-immunoreactive cells were observed in pallial and subpallial areas, paraventricular region, tuberal and retromammillary hypothalamic areas, posterior tubercle, prethalamic and thalamic areas, optic tectum, torus semicircularis, mesencephalic tegmentum, interpeduncular nucleus, superior and middle reticular nuclei, magnocellular vestibular nucleus, solitary tract nucleus, nucleus medianus magnocellularis, the spinal cord and amacrine cells in the retina. Large neurons in cranial nerve sensory ganglia were also labeled. The comparison of these results with those from other vertebrates, using a neuromeric analysis, reveals a conserved pattern of organization of the nitrergic system from this primitive fish group to amniotes, including mammals.

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Neuronal nitric oxide synthase in the olfactory system, forebrain, pituitary and retina of the adult teleost Clarias batrachus

Cited by 14 publications

References 87 publications

Pattern of Nitrergic Neuronal System Organization in the Brain of Two Holostean Fishes (Actinopterygii: Ginglymodi)

Pattern of Nitrergic Neuronal System Organization in the Brain of Two Holostean Fishes (Actinopterygii: Ginglymodi)

First description of an olfactory neuroblastoma in goldfish Carassius auratus: a case report

Organization of the nitrergic neuronal system in the primitive bony fishesPolypterus senegalusandErpetoichthys calabaricus(Actinopterygii: Cladistia)

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