Neurons from substantia nigra increase the efficacy and potency of second messenger arising from striatal astroglia dopamine receptor

Hanssoń, Elisabeth; Rönnbäck, Lars

doi:10.1002/glia.440010606

Cited by 25 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…*P Ͻ 0.05. Rönnbäck, 1988). Previous works have also found a positive correlation between the entity of dopaminedependent cAMP formation in cultured astrocytes and the dopaminergic innervation of the brain areas of the astrocytes (Henn et al, 1977;Hansson and Rönnbäck, 1988).…”

Section: Discussionmentioning

confidence: 88%

Pharmacological and molecular evidence for dopamine D1 receptor expression by striatal astrocytes in culture

et al. 1999

View full text Add to dashboard Cite

The neurotransmitter dopamine (DA) at a 10 microM concentration elicited a stimulation of intracellular cyclic AMP (cAMP) accumulation in cultured astrocytes derived from embryonic rat striatum. This accumulation was partially blocked by the beta-adrenergic receptors antagonist propranolol, mimicked by the D(1) agonist SKF 38393 and by the mixed D(1)/D(2) agonist apomorphine. A regional heterogeneity in the magnitude of dopamine-induced cAMP accumulation was observed in cultured astrocytes obtained from different brain areas. The maximum effect was observed in striatal astrocytes, a lower effect in cortical astrocytes, and no increase was detected in cerebellar astrocytes. Reverse transcription-polymerase chain reaction (RT-PCR) coupled to Southern blot hybridization demonstrated that striatal astrocytes express only D(1) receptor mRNA and Western blot analysis confirmed the expression of the D(1) receptor protein in striatal astrocytes. In contrast to what found in neurons, the D(1)-dependent cAMP formation in striatal astrocytes is partially reduced by pertussis toxin (PTX) treatment. The stimulation of D(1) receptors or the activation of adenylyl cyclase by forskolin led to an increase of cytosolic and nuclear protein kinase A (PKA) catalytic activity. The presence of dopamine D(1) receptors in cultured striatal astrocytes suggests a role of dopamine in the regulation of cellular processes in striatal astrocytes.

show abstract

Section: Discussionmentioning

confidence: 88%

Pharmacological and molecular evidence for dopamine D1 receptor expression by striatal astrocytes in culture

et al. 1999

View full text Add to dashboard Cite

show abstract

“…These data suggest that dopamine induced transmigration of CD14 + CD16 + monocytes across the BBB involves the activation of D1-like dopamine receptors on CD14 + CD16 + monocytes. BMVEC and astrocytes also express D1-like dopamine receptors (Hansson and Ronnback, 1988; Bacic et al, 1991). The possible contribution of activation of D1-like dopamine receptors on the cells of the BBB in dopamine induced CD14 + CD16 + monocyte transmigration will be examined in future studies.…”

Section: Resultsmentioning

confidence: 99%

Dopamine Increases CD14+CD16+ Monocyte Transmigration across the Blood Brain Barrier: Implications for Substance Abuse and HIV Neuropathogenesis

Calderon

Williams

Lopez

et al. 2017

J Neuroimmune Pharmacol

View full text Add to dashboard Cite

In human immunodeficiency virus-1 (HIV) infected individuals, substance abuse may accelerate the development and/or increase the severity of HIV associated neurocognitive disorders (HAND). It is proposed that CD14+CD16+ monocytes mediate HIV entry into the central nervous system (CNS) and that uninfected and infected CD14+CD16+ monocyte transmigration across the blood brain barrier (BBB) contributes to the establishment and propagation of CNS HIV viral reservoirs and chronic neuroinflammation, important factors in the development of HAND. The effects of substance abuse on the frequency of CD14+CD16+ monocytes in the peripheral circulation and on the entry of these cells into the CNS during HIV neuropathogenesis are not known. PBMC from HIV infected individuals were analyzed by flow cytometry and we demonstrate that the frequency of peripheral blood CD14+CD16+ monocytes in HIV infected substance abusers is increased when compared to those without active substance use. Since drug use elevates extracellular dopamine concentrations in the CNS, we examined the effects of dopamine on CD14+CD16+ monocyte transmigration across our in vitro model of the human BBB. The transmigration of this monocyte subpopulation is increased by dopamine and the dopamine receptor agonist, SKF 38393, implicating D1-like dopamine receptors in the increase in transmigration elicited by this neurotransmitter. Thus, elevated extracellular CNS dopamine may be a novel common mechanism by which active substance use increases uninfected and HIV infected CD14+CD16+ monocyte transmigration across the BBB. The influx of these cells into the CNS may increase viral seeding and neuroinflammation, contributing to the development of HIV associated neurocognitive impairments.

show abstract

“…For example, the glia of the projection area of mesencephalic dopaminergic neurons may be more important than the glia in the perikaryal area (Prochiantz et al, 1987). Conversely, glial development may be regulated by neuronal afferents from a natural projection area (Hansson and Ronnback, 1988). The alternative conclusion would be that certain features, e.g., functional properties, of neurons develop according to an inherent program, the realization of which is more dependent on the glial environment in one neuronal phenotype than another.…”

Section: Discussionmentioning

confidence: 99%

Region‐ and sex‐related differences in maturation of astrocytes in dissociated cell cultures of embryonic rat brain

Beyer

Epp

Fassberg

et al. 1990

Glia

View full text Add to dashboard Cite

Previous studies using dissociated cell cultures of fetal rat brain have revealed considerable regional diversity as well as sex steroid-independent sex differences in developmental schedules of dopaminergic neurons. Because these phenomena might be related to glial heterogeneity, cultures of dissociated male and female diencephalon, mesencephalon, and rhombencephalon of gestational day 14 rats were investigated with respect to the development of astrocytic markers. Cultures were incubated for 3-8 days in vitro (DIV) in serum-supplemented or serum-free medium. Vimentin and glial fibrillary acidic protein (GFAP) were quantified by counting of immunolabeled cells and immunoblotting. Vimentin and GFAP content rose from DIV 3 to 6 in all cultures. Regional variation of vimentin content was low, but large differences occurred in amounts of GFAP. GFAP reached high levels in rhombencephalon, especially when supplemented with serum, but remained very low or not detectable in mesencephalon. Simultaneous immunostaining for both cytoskeletal proteins revealed the presence of large numbers of vimentin single-labeled and small numbers of vimentin/GFAP double-labeled cells. Numbers of cells expressing GFAP showed similar regional variations as GFAP contents in both serum-free and serum-supplemented medium. They rose steeply from DIV 3 to 8 in rhomb- and diencephalon but not in mesencephalon. Transiently, female diencephalic cultures contained slightly more GFAP-immunoreactive cells than male cultures. The results thus demonstrate considerable regional heterogeneity of astrocytic maturation. However, neither the regional nor the sex differences show a consistent correlation with previous data on development of dopaminergic and other monoaminergic neurons in vitro. It seems likely that the dependence of neurons on glial environment for realization of an inherent developmental program varies among neuronal phenotypes.

show abstract

Neurons from substantia nigra increase the efficacy and potency of second messenger arising from striatal astroglia dopamine receptor

Cited by 25 publications

References 18 publications

Pharmacological and molecular evidence for dopamine D1 receptor expression by striatal astrocytes in culture

Pharmacological and molecular evidence for dopamine D1 receptor expression by striatal astrocytes in culture

Dopamine Increases CD14+CD16+ Monocyte Transmigration across the Blood Brain Barrier: Implications for Substance Abuse and HIV Neuropathogenesis

Region‐ and sex‐related differences in maturation of astrocytes in dissociated cell cultures of embryonic rat brain

Contact Info

Product

Resources

About