Neurons in the Rat Lateral Hypothalamic Area Integrate Information from the Gastric Vagal Nerves and the Cerebellar Interpositus Nucleus

Zhang, Yueping; Zhu, Jing-Ning; Chen, Kun; Li, Hongzhao; Wang, Jianjun

doi:10.1159/000088639

Cited by 18 publications

(28 citation statements)

References 37 publications

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“…The response patterns of VMN neurons were determined by comparing the discharge rate before and after the stimulation/administration. According to the previous established criteria (Min et al 1989;Zhang et al 2003Zhang et al , 2005Wen et al 2004;Zhu et al 2004Zhu et al , 2006aLi et al 2009), inhibition from stimulation was significant if the discharge frequency decreased at least to 30 % (70 % change) of the basal rate, and excitation was significant if the discharge frequency increased to at least 200 % (100 % change) of the basal firing level. Furthermore, statistical analyses were performed using Student's t test.…”

Section: Stimulation Electrophysiological Recording Data Acquisitiomentioning

confidence: 99%

Medial cerebellar nucleus projects to feeding-related neurons in the ventromedial hypothalamic nucleus in rats

Zhuang

Gao

et al. 2016

Brain Struct Funct

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The cerebellum, a hindbrain motor center, also participates in regulating nonsomatic visceral activities such as feeding control. However, the underlying neural mechanism is largely unknown. Here, we investigate whether the cerebellar medial nucleus (MN), one of the final outputs of the cerebellum, could directly project to and modulate the feeding-related neurons in the ventromedial hypothalamic nucleus (VMN), which has been traditionally implicated in feeding behavior, energy balance, and body weight regulation. The retrograde tracing results show that both GABAergic and glutamatergic projection neurons in the cerebellar MN send direct projections to the VMN. Electrical stimulation of cerebellar MN elicits an inhibitory, excitatory or biphasic response of VMN neurons. Interestingly, the VMN neurons modulated by cerebellar MN afferents not only receive phasic and tonic inputs from the gastric vagal nerves, but also are sensitive to peripheral glycemia and ghrelin signals. Moreover, a summation of inputs from the cerebellar MN and gastric vagal afferents occurs on single glycemia/ghrelin-sensitive neurons in the VMN, and the immunostaining result show that the axons from the cerebellar MN and the projections from the nucleus tractus solitarius, which conveys the gastric vagal inputs to hypothalamus, converge on single VMN glycemia/ghrelin-sensitive neurons. These results demonstrate that the somatic information forwarded by the cerebellar MN, together with the feeding signals from periphery, converge onto single VMN neurons, suggesting that a somatic-visceral integration related to feeding may occur in the VMN and the cerebellum may actively participate in the feeding regulation through the direct cerebellar MN-VMN projections.

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Section: Stimulation Electrophysiological Recording Data Acquisitiomentioning

confidence: 99%

Medial cerebellar nucleus projects to feeding-related neurons in the ventromedial hypothalamic nucleus in rats

Zhuang

Gao

et al. 2016

Brain Struct Funct

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“…LHA neuronal activity has been suggested to be closely associated with the onset of feeding, and inhibition of lateral hypothalamic neurons by glucose is sufficient to suppress feeding (Booth, 1968;Kurata et al, 1986;Bernardis and Bellinger, 1996;Zhang et al, 2005). Hence amylin's inhibition of dLHA neurons may be involved in the reduction of food intake after peripheral amylin.…”

Section: Functional Considerationsmentioning

confidence: 99%

Identification of central projections from amylin-activated neurons to the lateral hypothalamus

2010

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The ability of the pancreatic hormone amylin to inhibit food intake relies on a direct activation of the area postrema (AP). This activation is synaptically transmitted to the nucleus of the solitary tract (NTS), the lateral parabrachial nucleus (LPB), the central amygdaloid nucleus (Ce) and the lateral bed nucleus of stria terminalis (BSTL). Interestingly, neurons of the rostro-dorsal lateral hypothalamic area (dLHA), which are activated during fasting, are inhibited by peripheral amylin, although they lack amylin receptors. Using the retrograde tracer cholera toxin-B (Ctb) we analyzed whether the dLHA receives neuronal projections from amylin-activated brain areas. The anterograde tracer biotinylated dextran-amine (BDA) was used to confirm the projections and to identify further neuronal pathways potentially involved in amylin signaling. We identified dense projections from the amylin activated neurons in the LPB and sparse projections from the NTS to the dLHA. LPB fiber efferents were found in close proximity to dLHA nuclei activated by 24h of fasting. The AP and the Ce showed no projections to the dLHA. Dense efferents were also observed from the LPB to other hypothalamic areas, namely to the ventromedial, dorsomedial, paraventricular and arcuate nuclei. This study provides neuroanatomical evidence that among the amylin activated areas, the LPB provides the strongest input to the dLHA, thus it may mediate the amylin-induced inhibition of the dLHA. ABSTRACTThe ability of the pancreatic hormone amylin to inhibit food intake relies on a direct

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“…If the recorded DMN neuron responded to the cerebellar FN stimulation, the neuron was tested for its activity changes in response to 1 or more of the following stimuli in succession: (1) electrical stimulation of the gastric vagal nerves; (2) glycemia-sensitivity test [38,39] , that is, intravenous administration of glucose (0.4 M , 0.5 ml/kg; Sigma), which moderately modified blood glucose in small amounts to simulate normal physiological glycemic fluctuations associated with feeding [39][40][41] ; (3) intravenous and ICA administrations of normal saline (0.9% NaCl, 0.5 ml/kg) and mannitol (0.4 M , 0.5 ml/kg; Sigma), used as a volumetric and an osmotic control, respectively [2,30,39,40,[42][43][44] ; (4) ICA injection of leptin (25 or 100 g/kg; Sigma) [34] , dissolved freshly before the experiment in sterile normal saline (0.9% NaCl). In some DMN neurons that responded to both cerebellar FN and gastric vagal stimulations, responses of the neurons to the simultaneous electrical stimulations of the cerebellar FN and gastric vagal nerves were further observed.…”

Section: Stimulation Electrophysiological Recording Data Acquisitiomentioning

confidence: 99%

“…The response patterns of DMN neurons were determined by comparing the discharge rate before and after the stimulation/administration. According to the previously established criteria [27,30,31,[42][43][44][45] , inhibition due to the stimulation was significant if the discharge rate in the responsive window decreased at least to 30% (70% change) of the basal rate in control window, and excitation was significant if the discharge rate increased to at least 200% (100% change) of the basal rate. Furthermore, statistical analyses were performed by using Student's t test.…”

Section: Stimulation Electrophysiological Recording Data Acquisitiomentioning

confidence: 99%

“…We conducted the glycemia-sensitivity tests on 70 DMN neurons that responded to the cerebellar FN stimulation. By use of the established criteria [30,38,42,43] that the hypothalamic glycemiasensitive neurons show a specific and significant response to the intravenous injection of glucose but no response to the administration of normal saline and mannitol, 49 (70%) neurons were identified to be glycemia sensitive (p ! 0.05; fig.…”

Section: Responses Of Dmn Neurons Receiving Cerebellar Fn Inputs To Imentioning

confidence: 99%

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Cerebellar Fastigial Nuclear Inputs and Peripheral Feeding Signals Converge on Neurons in the Dorsomedial Hypothalamic Nucleus

Li¹,

Guo²,

Tang³

et al. 2009

Neurosignals

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Previous studies have indicated that neurons in the dorsomedial hypothalamic nucleus (DMN) receive feeding-related signals from the gastric vagal nerves, glycemia as well as leptin. On the other hand, it is intriguing that the cerebellum participates in regulating nonsomatic visceral activities including food intake via the direct cerebellohypothalamic projections. The present study was designed to examine, by using extracellular recordings in vivo in rats, whether the cerebellar fastigial nucleus (FN) could reach and converge with the feeding-associated gastric vagal, glycemia and leptin signals onto single DMN neurons. Of the 200 DMN neurons recorded, 104 (52%) responded to the cerebellar FN stimulation, in which 95 (91.3%) were also responsive to the gastric vagal stimulation, suggesting a convergence of cerebellar FN and gastric vagal inputs on the DMN neurons. Moreover, a summation of responses was observed (n = 10) when the cerebellar FN and gastric vagal nerve were simultaneously stimulated. Among the 18 DMN neurons receiving convergent inputs from the cerebellar FN and gastric vagal nerves, 16 (88.9%) cells also responded to the systemic administrations of glucose and leptin. These results demonstrated that the cerebellar FN-afferent inputs, together with the feeding signals from the gastric vagal nerves, blood glucose as well as leptin, converge onto single DMN neurons, suggesting that a somatic-visceral integration related to the feeding may occur in the DMN and the cerebellum may actively participate in the feeding regulation through the cerebellar FN-DMN projections.

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Neurons in the Rat Lateral Hypothalamic Area Integrate Information from the Gastric Vagal Nerves and the Cerebellar Interpositus Nucleus

Cited by 18 publications

References 37 publications

Medial cerebellar nucleus projects to feeding-related neurons in the ventromedial hypothalamic nucleus in rats

Medial cerebellar nucleus projects to feeding-related neurons in the ventromedial hypothalamic nucleus in rats

Identification of central projections from amylin-activated neurons to the lateral hypothalamus

Cerebellar Fastigial Nuclear Inputs and Peripheral Feeding Signals Converge on Neurons in the Dorsomedial Hypothalamic Nucleus

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