Neuropeptide and neurohormone precursors in the pea aphid, <i>Acyrthosiphon pisum</i>

Huybrechts, Jurgen; Bonhomme, Joël; Minoli, Sebastián; Prunier-Leterme, Nathalie; Dombrovsky, Aviv; Abdel-latief, Mohatmed; Robichon, Alain; Veenstra, Jan A.; Tagu, Denis

doi:10.1111/j.1365-2583.2009.00951.x

Cited by 120 publications

(128 citation statements)

References 37 publications

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“…Adipokinetic hormone is synthesized in the glandular lobe of the CC, reviewed by Nassel and Winther [29], and consistent with this, AKH was identified from direct analyses of single CC by mass spectrometry. Furthermore, incompletely processed forms of AKH, extended at its C-terminus with the amidation and cleavage sites (GK and GKR), were also present in the CC.…”

Section: Discussionmentioning

confidence: 88%

“…Similar evaluations of various invertebrates have since been facilitated by the availability of other genome databases [4,16,17,27,29,57], and the recent publication of the genome of D. suzukii has now enabled a similar evaluation of the peptidome of this invasive pest. A comparison between the peptides and precursors of D. suzukii and D. melanogaster show they are highly conserved.…”

Section: Discussionmentioning

confidence: 99%

“…This tetrapeptide itself is not present in insects, only N-terminally extended FMRFamides have been identified to date. Furthermore, although a variable number of peptides are predicted from the FMRFamide-like peptide precursors of insects, it is only in flies [5,46] and aphids [16,29] where peptides with a C-terminal FMRFamide motif have been identified. In extracts of nervous tissues from D. suzukii, FMRFamides were only found in the brain and TAG, and do not appear to be present in the CC of this insect.…”

Section: Discussionmentioning

confidence: 99%

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Genomic and peptidomic analyses of the neuropeptides from the emerging pest, Drosophila suzukii

2015

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Tel: +44 (0)1904 4626282 Highlights  In silico analysis identified 28 peptide precursors  Peptidome analysis of adult brain, corpus cardiacum, thoracico-abdominal ganglion identified peptides from 15 different families.  Myosuppressin was present in all tissue analysed, and was the only peptide identified in the crop nerve bundle AbstractDrosophila suzukii is a highly polyphagous invasive pest which has been recently introduced into Europe and North America, where it is causing severe economic losses through larval infestations of stone and berry fruits. The peptidome of the selected nervous tissues of adult D. suzukii was investigated as a first step in identifying potential targets for the development of novel insecticides.Through in silico analyses of the D. suzukii genome databases 28 neuropeptide families, comprising more than 72 predicted peptides were identified. Using a combination of liquid chromatography and mass spectrometry of tissue extracts, 33 predicted peptides, representing 13 different peptide families were identified by their molecular masses and a total of 17 peptide sequences were confirmed by ion fragmentation.A comparison between the peptides and precursors of D. suzukii and D. melanogaster shows they are highly conserved, with differences only identified in the amino acid sequences of the peptides encoded in the FMRFamide, hugin and ecydysis triggering hormone precursors. All other peptides predicted and identified from D. suzukii appear to be identical to those previously characterized from D. melanogaster.Adipokinetic hormone was only identified in the corpus cardiacum, other peptides present included short neuropeptide F, a pyrokinin and myosuppressin, the latter of which was the only peptide identified from the crop nerve bundle. Peptides present in extracts of the brain and/or thoracicoabdominal ganglion included allatostatins, cardioacceleratory peptide 2b, corazonin, extended FMRFamides, pyrokinins, myoinihibitory peptides, neuropeptide-like precursor 1, SIFamide, short neuropeptide F, kinin, sulfakinins and tachykinin related peptides.

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Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Genomic and peptidomic analyses of the neuropeptides from the emerging pest, Drosophila suzukii

2015

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“…These approaches have recently uncovered the FGLa/ASTs from the Hymenoptera, including the honey bee (Apis mellifera L., 1758) (Hummon et al 2006;Boerjan et al 2010), the parasitoid wasp Nasonia vitripennis (Walker, 1836) (Hauser et al 2010), and the Panamanian leafcutter ant (Acromyrmex echinatior (Forel, 1899)) (GenBank accession No. EGI57352); Hemiptera, including the pea aphid (Acrythosiphon pisum Harris, 1776) (Huybrechts et al 2010) and R. prolixus (Ons et al 2009(Ons et al , 2011; and Siphunculata, the human body louse (Pediculus humanus corporis) (cited in Weaver and Audsley 2009). Furthermore, FGL-amide ASTs have been uncovered in the genome of arachnids including the black-legged deer tick (Ixodes scapularis Say, 1821) (Christie 2008;Neupert et al 2009a) and the American dog tick (Dermacentor variabilis (Say, 1821)) (Bissinger et al 2011).…”

Section: The Fglamide-related-ast Familymentioning

confidence: 99%

Families of allatoregulator sequences: a 2011 perspective¹This review is part of a virtual symposium on recent advances in understanding a variety of complex regulatory processes in insect physiology and endocrinology, including development, metabolism, cold hardiness, food intake and digestion, and diuresis, through the use of omics technologies in the postgenomic era.

BendenaWilliam

TobeStephen

2012

Can. J. Zool.

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Three different peptide families have been named "allatostatins" (ASTs), based on their initial purifications which were based on their ability to inhibit juvenile hormone (JH) biosynthesis. These include (i) a family of peptides that have a consensus C-terminal sequence Y/FXFGL-NH 2 ; (ii) a family of peptides with a conserved C-terminal sequence W(X) 6 W-NH 2 ; and(iii) a family of peptides with C-terminal sequence PISCF, some of which are C-terminally-amidated. Each allatostatin family has functions distinct and apart from the inhibition of JH biosynthesis. A peptide family known as the "allatotropins" serve to stimulate JH biosynthesis. This family of peptides also has been proven to exert multiple effects dependent on the species in question. Genome and peptidome projects are uncovering new members of these families and it is clear that these structures are not just confined to Insecta but are found in a range of invertebrates. The receptors for these neuropeptides have been identified and tested experimentally for specific ligand binding. The Y/FXFGLa-ASTs exert their action through galanin-like receptors, W(X) 6 Wa-ASTs through a sex peptide-binding receptor, and PISCF-ASTs through somatostatin-like receptors. These receptors are conserved through evolutionary time and are being identified in numerous invertebrates by way of genome projects.Key words: allatostatin, allatotropin, juvenile hormone, muscle contraction, neuropeptides, neuropeptide receptors, insects, crustaceans, arthropods.Résumé : Trois familles différentes de peptides portent le nom d'« allatostatines » (AST) d'après leurs purifications initiales basées sur leur capacité d'inhiber la biosynthèse de l'hormone juvénile (JH). Elles incluent (i) une famille de peptides qui possèdent une séquence consensus C-terminale Y/FXFGL-NH 2 , (ii) une famille de peptides avec une séquence C-terminale conservée W(X) 6 W-NH 2 et (iii) une famille de peptides avec une séquence C-terminale PISCF, dont certains sont amidatées sur l'extrémité C-terminale. Chaque famille d'allatostatines possède des fonctions distinctes et additionnelles à l'inhibition de la synthèse de l'hormone juvénile. Une famille de peptides connus sous le nom d'« allatotropines » sert à stimuler la synthèse de la JH. Cette famille de peptides produit aussi des effets multiples selon l'espèce concernée. Des projets sur le génome et le peptidome découvrent de nouveaux membres de ces familles et il est clair que ces structures ne se retrouvent pas seulement chez les insectes, mais aussi chez une gamme d'invertébrés. Les récepteurs de ces neuropeptides ont été identifiés et ont été testé expérimentalement pour des liaisons spécifiques au ligand. Les AST-Y/FXFGLa exercent leur action par des récepteurs de type galanine, les AST-W(X)6Wa par un récepteur liant un peptide sexuel et les AST-PISCF par des récepteurs de type somatostatine. Ces récepteurs sont conservés au cours de l'évolution et sont présentement retrouvés chez de nombreux invertébrés dans le cadre des projets de génome.

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“…Other brain proteome studies have been conducted on the fleshfly Sarcophaga crassipalpis (Pavlides et al, 2011), aphid (Hummon et al, 2006;Huybrechts et al, 2010), the silkworm Bombyx mori (Li et al, 2007;Li et al, 2010;Li et al, 2009), and the honey bee (Uno et al, 2007;Wolschin et al, 2009). A differential proteomic study of diapausing and nondiapausing pupal brains of fleshfly have shown higher amounts of stress-related proteins but lower abundance of other metabolism-related brain proteins in the diapausal state (Li et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Brain Proteomics of Anopheles gambiae

Dwivedi

Muthusamy

Kumar

et al. 2014

OMICS: A Journal of Integrative Biology

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Anopheles gambiae has a well-adapted system for host localization, feeding, and mating behavior, which are all governed by neuronal processes in the brain. However, there are no published reports characterizing the brain proteome to elucidate neuronal signaling mechanisms in the vector. To this end, a large-scale mapping of the brain proteome of An. gambiae was carried out using high resolution tandem mass spectrometry, revealing a repertoire of >1800 proteins, of which 15% could not be assigned any function. A large proportion of the identified proteins were predicted to be involved in diverse biological processes including metabolism, transport, protein synthesis, and olfaction. This study also led to the identification of 10 GPCR classes of proteins, which could govern sensory pathways in mosquitoes. Proteins involved in metabolic and neural processes, chromatin modeling, and synaptic vesicle transport associated with neuronal transmission were predominantly expressed in the brain. Proteogenomic analysis expanded our findings with the identification of 15 novel genes and 71 cases of gene refinements, a subset of which were validated by RT-PCR and sequencing. Overall, our study offers valuable insights into the brain physiology of the vector that could possibly open avenues for intervention strategies for malaria in the future.

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Neuropeptide and neurohormone precursors in the pea aphid, Acyrthosiphon pisum

Cited by 120 publications

References 37 publications

Genomic and peptidomic analyses of the neuropeptides from the emerging pest, Drosophila suzukii

Genomic and peptidomic analyses of the neuropeptides from the emerging pest, Drosophila suzukii

Brain Proteomics of Anopheles gambiae

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