Neuropeptides predicted from the transcriptome analysis of the gray garden slug Deroceras reticulatum

Ahn, Seung‐Joon; Martin, Ruth C.; Rao, Sujaya; Choi, Man‐Yeon

doi:10.1016/j.peptides.2017.05.005

Cited by 52 publications

(81 citation statements)

References 87 publications

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“…The open reading frames (ORF) of allatostatin A1 and A2 cDNA sequences encoded deduced proteins of 114 and 129 amino acids with theoretical molecular mass of 12.60 and 14.06 kDa, respectively. The peptide precursor contains GxLamide at the C-terminal end, which is in agreement with a previous report on allatostatins A in D. reticulatum and P. yessoensis (Ahn et al 2017;Zhang et al 2018). Phylogenetic analysis revealed that buccalin precursors A1 and A2 of H. discus hannai were most closely related to D. reticulatum buccalin A1 and C. virginica buccalinlike neuropeptide precursors, respectively (Figure 1).…”

Section: Allatostatin a Or Buccalinsupporting

confidence: 91%

“…In H. discus hannai, allatostatin B was identified. It was found to encode a 25-residue signal peptide followed by four putative allatostatin B peptides that contained conserved W residues at both ends, consistent with the results of previous studies (Ahn et al 2017;Zhang et al 2018).…”

Section: Allatostatin Bsupporting

confidence: 89%

“…This neuropeptide was first isolated from Aplysia californica (Miller et al 1993). It has been also reported in L. gigantea (Veenstra 2010), C. gigas (Stewart et al 2014), D. reticulatum (Ahn et al 2017), and P. yessoensis (Zhang et al 2018). In H. discus hannai, two buccalin transcripts, allatostatin A1 and A2, were identified that encode 24 and 30-residue signal peptides and 3 and 5 copies of the mature peptide, respectively.…”

Section: Allatostatin a Or Buccalinmentioning

confidence: 84%

“…This neuropeptide was first reported as an insect metabolic neuropeptide involved in the mobilization of energy substrates during physiological activities (Gäde 2009). In mollusks, AKH has been identified in Crassostrea gigas (Dubos et al 2017) and D. reticulatum (Ahn et al 2017). An 84-residue AKH precursor protein was identified that had a predicted N-terminal signal peptide and an AKH amidated peptide (pQVSFSTNWGSamide).…”

Section: Adipokinetic Hormone (Akh)mentioning

confidence: 99%

“…Two peptides of the vasopressin-oxytocin family have been identified and characterized in marine gastropod mollusks (Cruz et al 1987). In addition, neuropeptides have been identified from Lottia gigantea (Veenstra 2010), Deroceras reticulatum (Ahn et al 2017), Charonia tritonis (Bose et al 2017), and Patinopecten yessoensis (Zhang et al 2018), all of which provide valuable information to understand the mechanisms of neuroendocrine regulation in mollusks.…”

Section: Introductionmentioning

confidence: 99%

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In silico prediction of neuropeptides from the neural ganglia of Pacific abalone Haliotis discus hannai (Mollusca: Gastropoda)

Sharker

Sukhan

Kim

et al. 2020

The European Zoological Journal

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Neuropeptides play a central role in the regulation of reproduction, growth, development, and various other physiological processes. In our study, 26 neuropeptide precursors were identified from the pleuropedal and cerebral ganglia of Pacific abalone using transcriptome analysis. Of them, two (neuromacin and neuroglian) were potentially novel and two (myomodulin and FMRFamide) were involved in the reproductive regulation of mollusks. A BLAST search indicated that most of these neuropeptides exhibited the highest homologies with neuropeptides of other protostomian species. Myomodulin, FMRFamide, and molluscan insulin-related peptides shared 87%, 72%, and 88% sequence identities with homologous peptides of the tropical gastropod mollusk, Haliotis asinina. In silico analysis revealed that these identified neuropeptide precursors were likely to be extracellular secreted proteins. Alignment of multiple cerebrin, FFamide, and insulin-related peptide sequences illustrated that most of their biologically active residues were highly conserved with other invertebrate homologous neuropeptide residues. Three-dimensional (3D) structures of adipokinetic hormone, allatostain (A, B, and C), allatotropin, cerebrin, clionin, conopressin, elevenin, and neuromycin precursors in H. discus hannai exhibited a helix-loophelix structure. Based on phylogenetic analysis, buccalin A1 and A2, allatostain B, and FCAP neuropeptide precursors of Pacific abalone formed a clade with other gastropod and bivalve mollusks neuropeptide precursors. This study provides a novel insight for further functional studies of abalone and other gastropod mollusks.

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Section: Allatostatin a Or Buccalinsupporting

confidence: 91%

Section: Allatostatin Bsupporting

confidence: 89%

Section: Allatostatin a Or Buccalinmentioning

confidence: 84%

Section: Adipokinetic Hormone (Akh)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In silico prediction of neuropeptides from the neural ganglia of Pacific abalone Haliotis discus hannai (Mollusca: Gastropoda)

Sharker

Sukhan

Kim

et al. 2020

The European Zoological Journal

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Identification and localization of a gonadotropin‐releasing hormone‐related neuropeptide in Biomphalaria, an intermediate host for schistosomiasis

Rosa-Casillas

Jesús

Rodríguez³

et al. 2021

J of Comparative Neurology

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Freshwater snails of the genus Biomphalaria serve as obligatory hosts for the digenetic trematode Schistosoma mansoni, the causative agent for the most widespread form of intestinal schistosomiasis. Within Biomphalaria, S. mansoni larvae multiply and transform into the cercariae form that can infect humans. Trematode development and proliferation is thought to be facilitated by modifications of host behavior and physiological processes, including a reduction of reproduction known as “parasitic castration.” As neuropeptides participate in the control of reproduction across phylogeny, a neural transcriptomics approach was undertaken to identify peptides that could regulate Biomphalaria reproductive physiology. The present study identified a transcript in Biomphalaria alexandrina that encodes a peptide belonging to the gonadotropin‐releasing hormone (GnRH) superfamily. The precursor and the predicted mature peptide, pQIHFTPDWGNN‐NH2 (designated Biom‐GnRH), share features with peptides identified in other molluscan species, including panpulmonates, opisthobranchs, and cephalopods. An antibody generated against Biom‐GnRH labeled neurons in the cerebral, pedal, and visceral ganglia of Biomphalaria glabrata. GnRH‐like immunoreactive fiber systems projected to all central ganglia. In the periphery, immunoreactive material was detected in the ovotestis, oviduct, albumen gland, and nidamental gland. As these structures serve crucial roles in the production, transport, nourishment, and encapsulation of eggs, disruption of the GnRH system of Biomphalaria could contribute to reduced reproductive activity in infected snails.

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FMRF‐NH₂‐related neuropeptides in Biomphalaria spp., intermediate hosts for schistosomiasis: Precursor organization and immunohistochemical localization

Rolón‐Martínez

Habib

Mansour

et al. 2021

J of Comparative Neurology

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Freshwater snails of the genus Biomphalaria serve as intermediate hosts for the digenetic trematode Schistosoma mansoni, the etiological agent for the most widespread form of intestinal schistosomiasis. As neuropeptide signaling in host snails can be altered by trematode infection, a neural transcriptomics approach was undertaken to identify peptide precursors in Biomphalaria glabrata, the major intermediate host for S. mansoni in the Western Hemisphere. Three transcripts that encode peptides belonging to the FMRF‐NH2‐related peptide (FaRP) family were identified in B. glabrata. One transcript encoded a precursor polypeptide (Bgl‐FaRP1; 292 amino acids) that included eight copies of the tetrapeptide FMRF‐NH2 and single copies of FIRF‐NH2, FLRF‐NH2, and pQFYRI‐NH2. The second transcript encoded a precursor (Bgl‐FaRP2; 347 amino acids) that comprised 14 copies of the heptapeptide GDPFLRF‐NH2 and 1 copy of SKPYMRF‐NH2. The precursor encoded by the third transcript (Bgl‐FaRP3; 287 amino acids) recapitulated Bgl‐FaRP2 but lacked the full SKPYMRF‐NH2 peptide. The three precursors shared a common signal peptide, suggesting a genomic organization described previously in gastropods. Immunohistochemical studies were performed on the nervous systems of B. glabrata and B. alexandrina, a major intermediate host for S. mansoni in Egypt. FMRF‐NH2‐like immunoreactive (FMRF‐NH2‐li) neurons were located in regions of the central nervous system associated with reproduction, feeding, and cardiorespiration. Antisera raised against non‐FMRF‐NH2 peptides present in the tetrapeptide and heptapeptide precursors labeled independent subsets of the FMRF‐NH2‐li neurons. This study supports the participation of FMRF‐NH2‐related neuropeptides in the regulation of vital physiological and behavioral systems that are altered by parasitism in Biomphalaria.

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Neuropeptides predicted from the transcriptome analysis of the gray garden slug Deroceras reticulatum

Cited by 52 publications

References 87 publications

In silico prediction of neuropeptides from the neural ganglia of Pacific abalone Haliotis discus hannai (Mollusca: Gastropoda)

In silico prediction of neuropeptides from the neural ganglia of Pacific abalone Haliotis discus hannai (Mollusca: Gastropoda)

Identification and localization of a gonadotropin‐releasing hormone‐related neuropeptide in Biomphalaria, an intermediate host for schistosomiasis

FMRF‐NH₂‐related neuropeptides in Biomphalaria spp., intermediate hosts for schistosomiasis: Precursor organization and immunohistochemical localization

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Neuropeptides predicted from the transcriptome analysis of the gray garden slug Deroceras reticulatum

Cited by 52 publications

References 87 publications

In silico prediction of neuropeptides from the neural ganglia of Pacific abalone Haliotis discus hannai (Mollusca: Gastropoda)

In silico prediction of neuropeptides from the neural ganglia of Pacific abalone Haliotis discus hannai (Mollusca: Gastropoda)

Identification and localization of a gonadotropin‐releasing hormone‐related neuropeptide in Biomphalaria, an intermediate host for schistosomiasis

FMRF‐NH2‐related neuropeptides in Biomphalaria spp., intermediate hosts for schistosomiasis: Precursor organization and immunohistochemical localization

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FMRF‐NH₂‐related neuropeptides in Biomphalaria spp., intermediate hosts for schistosomiasis: Precursor organization and immunohistochemical localization