Neuroprotective effects of collagen matrix in rats after traumatic brain injury

Shin, Samuel S.; Grandhi, Ramesh; Henchir, Jeremy; Yan, Hong; Badylak, Stephen F.; Dixon, C. Edward

doi:10.3233/rnn-140430

Cited by 5 publications

(4 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When Lighthall and his colleagues first developed the CCI model, the device was powered by pressurized gas (i.e., pneumatically driven). Pneumatic CCI remains widely used to study TBI pathophysiology and test novel therapies ( 43 – 45 ). A typical pneumatic CCI device (Figure 1 ) includes a cylinder, which is rigidly mounted to a crossbar.…”

Section: Past and Present Applications: Model Overview Development mentioning

confidence: 99%

The Controlled Cortical Impact Model: Applications, Considerations for Researchers, and Future Directions

2016

Self Cite

View full text Add to dashboard Cite

Controlled cortical impact (CCI) is a mechanical model of traumatic brain injury (TBI) that was developed nearly 30 years ago with the goal of creating a testing platform to determine the biomechanical properties of brain tissue exposed to direct mechanical deformation. Initially used to model TBIs produced by automotive crashes, the CCI model rapidly transformed into a standardized technique to study TBI mechanisms and evaluate therapies. CCI is most commonly produced using a device that rapidly accelerates a rod to impact the surgically exposed cortical dural surface. The tip of the rod can be varied in size and geometry to accommodate scalability to difference species. Typically, the rod is actuated by a pneumatic piston or electromagnetic actuator. With some limits, CCI devices can control the velocity, depth, duration, and site of impact. The CCI model produces morphologic and cerebrovascular injury responses that resemble certain aspects of human TBI. Commonly observed are graded histologic and axonal derangements, disruption of the blood–brain barrier, subdural and intra-parenchymal hematoma, edema, inflammation, and alterations in cerebral blood flow. The CCI model also produces neurobehavioral and cognitive impairments similar to those observed clinically. In contrast to other TBI models, the CCI device induces a significantly pronounced cortical contusion, but is limited in the extent to which it models the diffuse effects of TBI; a related limitation is that not all clinical TBI cases are characterized by a contusion. Another perceived limitation is that a non-clinically relevant craniotomy is performed. Biomechanically, this is irrelevant at the tissue level. However, craniotomies are not atraumatic and the effects of surgery should be controlled by including surgical sham control groups. CCI devices have also been successfully used to impact closed skulls to study mild and repetitive TBI. Future directions for CCI research surround continued refinements to the model through technical improvements in the devices (e.g., minimizing mechanical sources of variation). Like all TBI models, publications should report key injury parameters as outlined in the NIH common data elements (CDEs) for pre-clinical TBI.

show abstract

Section: Past and Present Applications: Model Overview Development mentioning

confidence: 99%

The Controlled Cortical Impact Model: Applications, Considerations for Researchers, and Future Directions

2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…Specific religious groups and animal activists may object to the use of xeno-products and thus synthetic biomaterials can provide an ethically viable alternative. DuraGen Plus TM is reported to be minimally immunogenic, whilst also being compatible and mouldable for versus sham injury rats (130). Although this study shows promise for TBI, currently there are no studies which investigate DuraGen Plus TM 's efficacy in pTBI; the cellular responses of neural cells to this material have also not been documented in detail.…”

Section: Limitations In Relation To Hydrogel Testingmentioning

confidence: 95%

518 New In Vitro Model of Traumatic Brain Injury to Assess Biomaterial Based Regenerative Strategies

Basit

Jenkins

Chari

2021

British Journal of Surgery

View full text Add to dashboard Cite

Introduction Penetrating traumatic brain injury (pTBI) management is largely supportive, with no clinically established regenerative therapies. Neurocompatible biomaterials offer a high potential to promote regenerative mechanisms but facile, high throughput, pathomimetic in vitro pTBI models for the developmental testing of neuro-materials is lacking. Method A mouse mixed glial culture system was utilised within which penetrating injuries could be induced. DuraGen PlusTM – an FDA approved neurosurgical grade biomaterial could be implanted into lesions to assess cell-biomaterial responses. Reactive gliosis (astrocytic morphological responses/GFAP expression) and microglial infiltration (Iba1 expression) were assessed/quantified. Results Key pathological features of pTBI were observed in the model, with the ability to (i) introduce reproducible lesions (diameter 949 ± 26 μm) and (ii) for DuraGen PlusTM to be implanted into lesions. Peri-lesional astrocytes displayed hypertrophic palisading morphologies and GFAP upregulation, analogous to gliosis in vivo. Significant microglial numbers infiltrated the DuraGen PlusTM implant at 7 days post-lesion (132.41 ± 15.83 cells/mm2) versus lesion only (82.04 ± 5.11 cells/mm2), p < 0.05). Conclusions We have developed a novel, neuropathomimetic pTBI model, wherein biomaterial implantation enables investigation of neural cell-biomaterial responses. This model can facilitate early-stage evaluation of novel biomaterials as high throughput, inexpensive and facile screening tool.

show abstract

“…Collagen peptides were shown to enhance neurogenesis in the HC and reduce anxiety-like behavior in mice [59]. Research demonstrated that implanting collagen can be used to remediate traumatic brain injury by supporting neural regeneration and reducing cognitive loss [60]. It is interesting that, in mice, the HC contrasts with the ACC in collagen expression.…”

Section: Common Pathwaymentioning

confidence: 99%

Molecular Mechanisms for Changing Brain Connectivity in Mice and Humans

Voelker,

Weible,

Niell

et al. 2023

IJMS

View full text Add to dashboard Cite

The goal of this study was to examine commonalities in the molecular basis of learning in mice and humans. In previous work we have demonstrated that the anterior cingulate cortex (ACC) and hippocampus (HC) are involved in learning a two-choice visuospatial discrimination task. Here, we began by looking for candidate genes upregulated in mouse ACC and HC with learning. We then determined which of these were also upregulated in mouse blood. Finally, we used RT-PCR to compare candidate gene expression in mouse blood with that from humans following one of two forms of learning: a working memory task (network training) or meditation (a generalized training shown to change many networks). Two genes were upregulated in mice following learning: caspase recruitment domain-containing protein 6 (Card6) and inosine monophosphate dehydrogenase 2 (Impdh2). The Impdh2 gene product catalyzes the first committed step of guanine nucleotide synthesis and is tightly linked to cell proliferation. The Card6 gene product positively modulates signal transduction. In humans, Card6 was significantly upregulated, and Impdh2 trended toward upregulation with training. These genes have been shown to regulate pathways that influence nuclear factor kappa B (NF-κB), a factor previously found to be related to enhanced synaptic function and learning.

show abstract

Neuroprotective effects of collagen matrix in rats after traumatic brain injury

Cited by 5 publications

References 30 publications

The Controlled Cortical Impact Model: Applications, Considerations for Researchers, and Future Directions

The Controlled Cortical Impact Model: Applications, Considerations for Researchers, and Future Directions

518 New In Vitro Model of Traumatic Brain Injury to Assess Biomaterial Based Regenerative Strategies

Molecular Mechanisms for Changing Brain Connectivity in Mice and Humans

Contact Info

Product

Resources

About