Neurotoxic effect of 2,5-hexanedione on neural progenitor cells and hippocampal neurogenesis

Kim, Min-Sun; Park, Hee Ra; Park, Mi-Kyung; Kim, So Jung; Kwon, Mu-Gil; Yu, Byung Pal; Chung, Hae Young; Kim, Hyung Sik; Kwack, Seung Jun; Kang, Tae Seok; Kim, Seung Hee; Lee, Joo Young

doi:10.1016/j.tox.2009.03.013

Cited by 27 publications

(29 citation statements)

References 47 publications

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“…It has been reported that n-hexane exerted its cytotoxic effects by generating excess ROS, which could suppress neurogenesis and the proliferation of neural progenitor cells [46,47]. Oxidative damage could result in neuronal cell death and growth inhibition by hampering the normal function of the glial cells [48].…”

Section: Discussionmentioning

confidence: 99%

Effects of 2,5-hexanedione on angiogenesis and vasculogenesis in chick embryos

Cheng

Luo

Wang

et al. 2015

Reproductive Toxicology

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Section: Discussionmentioning

confidence: 99%

Effects of 2,5-hexanedione on angiogenesis and vasculogenesis in chick embryos

Cheng

Luo

Wang

et al. 2015

Reproductive Toxicology

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“…Therefore, the suppressive effects of capsaicin on NPC proliferation were evaluated in vivo by examining hippocampal neurogenesis, which occurs throughout life in adult mammals. Hippocampal neurogenesis decreases with aging (Kuhn et al, 1996) and is affected by environmental toxins such as the gamma-diketone compound 2,5-HD and acrylamide (Kim et al, 2009b;Park et al, 2010). It is of interest that environmental pollutants, endocrine-disrupting chemicals, and food additives were reported to affect developing embryonic and hippocampal neurogenesis (Kim et al, 2009a;Kreiling et al, 2000;Park et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Capsaicin Impairs Proliferation of Neural Progenitor Cells and Hippocampal Neurogenesis in Young Mice

Kong

Kim²,

Song³

et al. 2010

Journal of Toxicology and Environmental Health, Part A

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Capsaicin (N-vanillyl-8-methyl-1-nonenamide) is a major pungent ingredient in hot peppers and induces apoptosis in malignant carcinoma cell lines. However, the adverse effects of capsaicin on neuronal development have not been fully explored. The aim of this study was to determine whether capsaicin affected murine-derived cerebellar multi-potent neural progenitor cells (NPC) or adult hippocampal neurogenesis in vivo. Capsaicin dose-dependently suppressed NPC proliferation, and higher concentrations were cytotoxic. Capsaicin decreased the activation of extracellular signal-regulated kinases (ERK) without markedly affecting p38 kinases. Capsaicin reduced the number of newly generated cells in the dentate gyrus of the hippocampus but did not significantly alter learning and memory performance in young adult mice. Interestingly, capsaicin decreased ERK activation in the hippocampus, suggesting that reduced ERK signaling may be involved in the capsaicin-mediated regulation of hippocampal neurogenesis.

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“…The chemical is known to be a potent neurotoxicant and chronic exposure to it leads to severe neuropathy in humans and experimental animals1, 2 ) . Metabolism studies have demonstrated that 2,5-hexanedione (HD), a metabolite of n-hexane, is the causative agent in n-hexane-induced neurotoxicity1, 3 ) . Several studies showed that HD caused neuron loss.…”

Section: Introductionmentioning

confidence: 99%

“…Kim et al. 3 ) reported that HD decreased viability of C17.2 cells as multipotent neural progenitor cells (NPC) isolated from neonatal mouse cerebellum and reduced numbers of newly generated cells in the hippocampus of mice. Moreover, it was indicated that the mechanism of apoptosis may be involved in HD-induced inhibition of NPC proliferation and hippocampal neurogenesis.…”

Section: Introductionmentioning

confidence: 99%

2,5-hexanedione induced apoptosis in mesenchymal stem cells from rat bone marrow via mitochondria-dependent caspase-3 pathway

et al. 2015

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2,5-hexanedione (HD) induces apoptosis of nerve cells. However,the mechanism of HD-induced apoptosis remains unknown. Mesenchymal stem cells (MSCs) are multipotential stem cells with the ability to differentiate into various cell types. This study is designed to investigate the apoptosis induced by HD in rat bone marrow MSCs (BMSCs) and the related underlying mechanisms. The fifth generation of MSCs was treated with 0, 10, 20 and 40 mM HD respectively. The viability of BMSCs was observed by MTT. Apoptosis were estimated by Hoechst 33342 staining and TUNEL assay. The disruption of mitochondrial transmembrane potential (MMP) was examined by JC-1 staining. Moreover, the expression of Bax and Bcl-2, cytochrome c release, and caspase-3 activity were determined by real-time RT-PCR, Western blot and Spectrophotometry. Our results showed that HD induced apoptosis in MSCs in a dose dependent manner. Moreover, HD downregulated the Bcl-2 expression,upregulated the Bax expression and the Bax/Bcl-2 ratio, promoted the disruption of MMP, induced the release of cytochrome c from mitochondria to cytosol, and increased the activity of caspase-3 in MSCs. These results indicate that HD induces apoptosis in MSCs and the activated mitochondria-dependent caspase-3 pathway may be involved in the HD-induced apoptosis.

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Neurotoxic effect of 2,5-hexanedione on neural progenitor cells and hippocampal neurogenesis

Cited by 27 publications

References 47 publications

Effects of 2,5-hexanedione on angiogenesis and vasculogenesis in chick embryos

Effects of 2,5-hexanedione on angiogenesis and vasculogenesis in chick embryos

Capsaicin Impairs Proliferation of Neural Progenitor Cells and Hippocampal Neurogenesis in Young Mice

2,5-hexanedione induced apoptosis in mesenchymal stem cells from rat bone marrow via mitochondria-dependent caspase-3 pathway

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