Neurotropic peptide HLDF-6-amide reduces age-related decline in sexual activity in old male rats

Sokolov, O. Yu.; Прохорова, Т. А.; Tereshkina, Elena B.; Zozulya, S A; Симонов, А. Н.; Кост, Н. В.; Dadayan, A. K.; Bogachouk, A. P.; Zolotarev, Yu. A.

doi:10.1016/j.exger.2021.111329

Cited by 4 publications

(2 citation statements)

References 10 publications

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“…Upon returning to the lab at 28 weeks post-orchidectomy, none of the B6D2F1 exhibited ejaculatory reflex (Figure 1A). We speculated this could be reasons for extended social isolation with the absence of physical exposure to other mice (Liu et al ., 2019), minimal human handling (Cho et al ., 2021), or ageing (Sokolov et al ., 2021). In accordance with our values in animal ethics to reduce the use of animals, to generate more outcomes, justified euthanasia, and reduce waste (reviewed in (Smith, 2020)), we attempted to salvage this experiment drew from these speculations.…”

Section: Resultsmentioning

confidence: 99%

Neuron- and microglia-specific immunoexpression in steroid-independent male sexual behaviour in castrated B6D2F1 male mice

Chiang

Lipkin

McCusker

et al. 2022

Preprint

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Sexual behaviour is the sine qua non for procreation for several species and is traditionally viewed to be regulated by sex steroid hormones. However, several species exhibit steroid-independent sexual behaviour, and its molecular understanding is only beginning to be uncovered. We do not yet have a cell-specific understanding of the roles non-neuronal cells play in this phenomenon. Forty B6D2F1 hybrid male mice underwent orchidectomy and some of them exhibited consistent ejaculatory reflexes at least 62 weeks post-orchidectomy over 11 sexual behaviour tests (>180 minutes each), defined as steroid-independent (SI) maters (21.80%), and those that do not, defined as SI non-maters (25%). Using flow cytometry, we compared the preoptic area immunoexpression in NeuN+ neurons and Iba1+ microglia between SI maters and SI non-maters (n = 6 per group). We found neuronal immunoexpression up-regulated for amyloid precursor protein and androgen receptor, as well as down-regulated for glucocorticoid receptor. In conjunction, microglial immunoexpression of amyloid precursor protein up-regulated. These data suggest there are cell-specific immunoexpression changes including a role of non-neuronal cells in steroid-independent male sexual behaviour.

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Section: Resultsmentioning

confidence: 99%

Neuron- and microglia-specific immunoexpression in steroid-independent male sexual behaviour in castrated B6D2F1 male mice

Chiang

Lipkin

McCusker

et al. 2022

Preprint

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“…Современная терапия БП направлена на лечение последствий дегенерации дофаминовых нейронов, а не на неотъемлемую причину заболевания. Потенциальным кандидатом на роль нетоксичного и высокоэффективного препарата является пептид HLDF-6-H (Thr-Gly-Glu-Hse-His-Arg), гомосериновое производное фрагмента 41-46 фактора дифференцировки лейкоцитов человека HLDF [3]. Также было показано, что HLDF-6-H снижает вызванные старением воспалительные реакции у старых крыс.…”

Section: Introductionunclassified

HLDF-6-H Peptide Exhibits an Anti-inflammatory Effect in the Development of Depression in an MPTP Model of Parkinson's Disease

Zozulya,

Sokolov,

Kost

et al. 2023

BIOMEDICINE

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In this work, we investigate the anti-inflammatory effect of the HLDF-6-H peptide (Thr-Gly-Glu-HseHis-Arg) in a model of the presymptomatic stage of Parkinson’s disease (PD). This peptide is a bioactive fragment of the HLDF leukocyte differentiation factor. The presymptomatic stage of PD was modelled by introducing moderate doses of MPTP toxin to C57Bl/6 mice. HLDF-6-H was administered intranasally at a dose of 300 μg/kg daily for three weeks. The severity of depression was determined by the time of immobilization in a Porsolt test. The serum levels of 10 steroids were determined using MS analysis. The activity of inflammatory markers leukocyte elastase (LE) and α1-proteinase inhibitor (α1-PI) was determined using kinetic methods. The development of an inflammatory process under the influence of MPTP is evidenced by an increase in the activity of α1-PI; the anti-inflammatory effect of the peptide is manifested in a decreased activity of LE and α1-PI. At the same time, the immobilization time of mice in a Porsolt test positively correlated with α1-PI and LE, which may indicate the role of inflammation in the development of depression. A positive correlation between estradiol and LE was found in the general sample of mice. In the group of mice treated with the peptide, α1-PI correlated positively with corticosterone and negatively with estradiol and androstenedione. It can be assumed that the anti-inflammatory effect of the peptide is associated with the regulation of corticosteroids and sex hormones. Hence, the applied experimental model confirmed the development of a depressive-like syndrome and neuroendocrine disorders in the presymptomatic stage of PD. The long-term use of HLDF-6-H has antidepressant and anti-inflammatory effects.

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Effect of Neurotropic and Immunotropic Drugs on Leukocyte Elastase Activity In Vitro

Zozulya,

Sokolov,

Kost

2024

Bull Exp Biol Med

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Neurotropic peptide HLDF-6-amide reduces age-related decline in sexual activity in old male rats

Cited by 4 publications

References 10 publications

Neuron- and microglia-specific immunoexpression in steroid-independent male sexual behaviour in castrated B6D2F1 male mice

Neuron- and microglia-specific immunoexpression in steroid-independent male sexual behaviour in castrated B6D2F1 male mice

HLDF-6-H Peptide Exhibits an Anti-inflammatory Effect in the Development of Depression in an MPTP Model of Parkinson's Disease

Effect of Neurotropic and Immunotropic Drugs on Leukocyte Elastase Activity In Vitro

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