Neutral lipid enzyme electrode based on ion-sensitive field effect transistors

Nakako, Mamiko; Hanazato, Yoshio; Maeda, Mitsuo; Shiono, Satoru

doi:10.1016/0003-2670(86)80044-7

Cited by 48 publications

(8 citation statements)

References 7 publications

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“…Micro reactor packed with immobilized lipase has been used for triglyceride detection using ion sensitive field effective transistor (ISFET) [10]. Nakako et al have prepared an enzyme electrode for neutral lipid determination based on hydrogen ion-sensitive field effect transistor (pH-FET) [11]. Reddy [3].…”

Section: Introductionmentioning

confidence: 99%

Nanostructured cerium oxide film for triglyceride sensor

Solanki¹,

Dhand²,

Kaushik³

et al. 2009

Sensors and Actuators B: Chemical

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Section: Introductionmentioning

confidence: 99%

Nanostructured cerium oxide film for triglyceride sensor

Solanki¹,

Dhand²,

Kaushik³

et al. 2009

Sensors and Actuators B: Chemical

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“…Micro reactor packed with immobilized lipase (LIP) has been used for triglyceride detection using ion-sensitive-field-effective-transistor (ISFET) [20]. Nakako et al have prepared an enzyme electrode for neutral lipid determination based on hydrogen ion-sensitive field effect transistor (pH-FET) [21]. Reddy et al have fabricated porous silica based potentiometric pH dependant triglyceride biosensor [22].…”

mentioning

confidence: 99%

Polyaniline/Single‐Walled Carbon Nanotubes Composite Based Triglyceride Biosensor

et al. 2010

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Nanocomposite film comprising of polyaniline (PANI) and single walled carbon nanotubes (SWCNT) has been fabricated onto indium-tin-oxide (ITO) coated glass plate using electrophoretic technique. Co-immobilization of glycerol dehydrogenase (GDH) and lipase (LIP) has been done via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry to explore its application for triglyceride (tributyrin) sensing. Response studies have been done using linear sweep voltammetry revealing that LIP-GDH/PANI-SWCNT-TB/ITO bioelectrode can detect tributyrin in the range of 50 to 400 mg dL À1 with low Michaelis-Menten constant of 1.138 mM, improved response time of 12 s, high sensitivity as 4.28 10 À4 mA mg À1 dL and storage stability of about 13 weeks.

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“…One of the FET's was immobilized with lipase membrane. The wide concentration ranges of triglycerides were determined by the proposed electrode within a short response time of 2 min [45]. The electrode suffered few technological and fundamental problems including instability in the functional groups of the sensing layer and impurities of the semiconductor.…”

Section: Tg Biosensors Based On Electrolyte-insulatorsemiconductor Camentioning

confidence: 99%

Recent trends and perspectives in enzyme based biosensor development for the screening of triglycerides: a comprehensive review

Hooda

Gahlaut

Gothwal

2018

Artificial Cells, Nanomedicine, and Biotechnology

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Clinical manifestations of the elevated plasma triacylglycerol (TG) include a greater prevalence of atherosclerotic heart disease, acute pancreatitis, diabetes mellitus, hypertension, and ischemic vascular disease. Hence, these significant health troubles have attracted scientific attention for the precise detection of TG in biological samples. Numerous techniques have been employed to quantify TG over many decades, but biosensors hold the leading position owing to their superior traits such as highly specific recognition for target molecules, accuracy, minituarization, small sample requirement and rapid response. Enzyme-based electrochemical biosensors represent an instantaneous resolution for the foremost bottlenecks constraining laboratory prototypes to reach real time bedside applications. We highlight the choice of transducers and constructive strategies to design high-performance biosensor for the quantification of triglycerides in sera and early diagnosis of health problems related to it. In the present review, a small effort has been made to emphasize the significant role of enzymes, nanostructured metal oxides, graphene, conducting polypyrrole, nanoparticles, porous silicon, EISCAP and ENFET in enabling TG biosensors more proficient and taking a revolutionary step forward.

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Neutral lipid enzyme electrode based on ion-sensitive field effect transistors

Cited by 48 publications

References 7 publications

Nanostructured cerium oxide film for triglyceride sensor

Nanostructured cerium oxide film for triglyceride sensor

Polyaniline/Single‐Walled Carbon Nanotubes Composite Based Triglyceride Biosensor

Recent trends and perspectives in enzyme based biosensor development for the screening of triglycerides: a comprehensive review

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