Neutral liposomes containing crown ether-lipids as potential DNA vectors

Angelini, Guido; Pisani, Michela; Mobbili, Giovanna; Marini, Milvia; Gasbarri, Carla

doi:10.1016/j.bbamem.2013.06.003

Cited by 26 publications

(15 citation statements)

References 53 publications

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“…CSCs and VM are involved in cell plasticity, which is the capability of an aberrant population to ECs transdifferentiation (Figure 1) (92). VEGF receptors regulate expression of specific marker for ECs, such as VE-Cadherin (93).…”

Section: Signaling Pathways Of Cscs In Vmmentioning

confidence: 99%

Cancer Stem Cells and Its Role in Angiogenesis and Vasculogenic Mimicry in Gastrointestinal Cancers

et al. 2020

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Cancer stem cells (CSCs) are able to promote initiation, survival and maintenance of tumor growth and have been involved in gastrointestinal cancers (GICs) such as esophageal, gastric and colorectal. It is well known that blood supply facilitates cancer progression, recurrence, and metastasis. In this regard, tumor-induced angiogenesis begins with expression of pro-angiogenic molecules such as vascular endothelial growth factor (VEGF), which in turn lead to neovascularization and thus to tumor growth. Another pattern of blood supply is called vasculogenic mimicry (VM). It is a reminiscent of the embryonic vascular network and is carried out by CSCs that have the capability of transdifferentiate and form vascular-tube structures in absence of endothelial cells. In this review, we discuss the role of CSCs in angiogenesis and VM, since these mechanisms represent a source of tumor nutrition, oxygenation, metabolic interchange and facilitate metastasis. Identification of CSCs mechanisms involved in angiogenesis and VM could help to address therapeutics for GICs.

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Section: Signaling Pathways Of Cscs In Vmmentioning

confidence: 99%

Cancer Stem Cells and Its Role in Angiogenesis and Vasculogenic Mimicry in Gastrointestinal Cancers

et al. 2020

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“…[ 28 ] synthesized and characterized a series of cholesterol and amino acid-based cationic lipids through ester and ether linkages. It has been noticed that both ether- and amide-linked lipids are more stable than the carbamate- or ester-linked lipids and provided an improved gene transfer efficiency [ 29 , 30 , 31 , 32 , 33 ]. The linker of amide and ether bonds to cationic lipids might be attributed to a stable and highly efficient gene transfer system.…”

Section: Introductionmentioning

confidence: 99%

Novel Cholesterol-Based Cationic Lipids as Transfecting Agents of DNA for Efficient Gene Delivery

Huan

Wan

et al. 2015

IJMS

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The design, synthesis and biological evaluation of the cationic lipid gene delivery vectors based on cholesterol and natural amino acids lysine or histidine are described. Cationic liposomes composed of the newly synthesized cationic lipids 1a or 1b and neutral lipid DOPE (1,2-dioleoyl-l-α-glycero-3-phosphatidyl-ethanolamine) exhibited good transfection efficiency. pEGFP-N1 plasmid DNA was transferred into 293T cells by cationic liposomes formed from cationic lipids 1a and 1b, and the transfection activity of the cationic lipids was superior (1a) or parallel (1b) to that of the commercially available 3β-[N-(N',N'-dimethylaminoethyl)-carbamoyl] cholesterol (DC-Chol) derived from the same cholesterol backbone with different head groups. Combined with the results of agarose gel electrophoresis, transfection experiments with various molar ratios of the cationic lipids and DOPE and N/P (+/−) molar charge ratios, a more effective formulation was formed, which could lead to relatively high transfection efficiency. Cationic lipid 1a represents a potential agent for the liposome used in gene delivery due to low cytotoxicity and impressive gene transfection activity.

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“…In this area of interest, the self-assembled association of neutral liposomes (L), DNA and divalent metal cations (M 2+ ) in ternary L-DNA-M 2+ complexes able to transfect genetic materials into cells have been explored; [3][4][5][6][7] in this system the interaction between neutral lipids and DNA is mediated by metal cations that provide stability to the complexes. In order to improve the ability of neutral liposomes to complex DNA, our strategy was to develop liposomal gene delivery systems containing new synthetic lipids lacking in positive charge but capable of acting as effective cationic lipids.…”

Section: Introductionmentioning

confidence: 99%

Liposome-based gene delivery systems containing a steroid derivative: computational and small angle X-ray diffraction study

et al. 2015

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In this study, the structural properties and the phase behaviour of mixed composition neutral liposomes containing a functionalized steroid are reported. With the aim to design neutral liposomes able to coordinate cations and to complex DNA, we synthesized cholesteryl-2-(picolinamido)-phenylcarbamate (CHOLp) containing an N-aryl picolinamide group as chelating agent linked to the steroid structure via a carbamate moiety. The phase behaviour of mixtures of the functionalized cholesterol (CHOLp) and dioleoyl-phosphatidylcholine (DOPC) was investigated by means of X-ray diffraction. Simultaneously, atomistic molecular dynamics simulations of DOPC/CHOLp bilayers as a function of CHOLp molar fractions were carried out to investigate the specific effects of the polar steroid on the structural and dynamic properties of these zwitterionic bilayers. The molecular modelling studies have been performed both in absence and in presence of bivalent cations salts in order to assess the CHOLp ability to coordinate metal ions. The results show good stability of the resulting DOPC/CHOLp bilayers which is improved by the presence of salt. This is particularly evident at low amount of CHOLp where a high order of the lipid tails can be observed, suggesting stabilization of the corresponding DOPC liposomes.This feature can be ascribed to the polar nature and structural properties of the ligand. In fact, due to the presence of the aromatic moieties, CHOLp combines two different behaviours, namely a propensity to realize both intermolecular p-stacking interactions and cation-p bonding mainly evident in CaCl 2 . The last feature confirms for CHOLp a role as a cation-mediated complexation agent for DNA. The X-ray diffraction data on the capability of DOPC/CHOLp liposomes to complex DNA was also reported.

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Neutral liposomes containing crown ether-lipids as potential DNA vectors

Cited by 26 publications

References 53 publications

Cancer Stem Cells and Its Role in Angiogenesis and Vasculogenic Mimicry in Gastrointestinal Cancers

Cancer Stem Cells and Its Role in Angiogenesis and Vasculogenic Mimicry in Gastrointestinal Cancers

Novel Cholesterol-Based Cationic Lipids as Transfecting Agents of DNA for Efficient Gene Delivery

Liposome-based gene delivery systems containing a steroid derivative: computational and small angle X-ray diffraction study

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