Neutralization Epitopes on Poliovirus Type 3 Particles: an Analysis Using Monoclonal Antibodies

Ferguson, Morag; Minor, Philip D.; Magrath, D. I.; Yi-Hua, Qui; Spitz, M.; Schild, G. C.

doi:10.1099/0022-1317-65-1-197

Cited by 46 publications

(43 citation statements)

References 14 publications

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“…Monoclonal antibodies were prepared by fusion of splenocytes from immunized BALB/c mice and myeloma cells as previously described (Ferguson et at., 1984). Ascites were prepared in syngeneic mice.…”

Section: Methodsmentioning

confidence: 99%

“…Monoclonal antibodies which react with D (or N), C (or H) or both D and C (N and H) particles have been identified (Singer et al, 1989;Ferguson et al, 1984;Brioen et al, 1982). For type 3 virus it has been shown that amino acid sequences at antigenic sites on infectious virus are also exposed on the surface of non-infectious C antigen but in a different conformation (Ferguson & Minor, 1990).…”

Section: Introductionmentioning

confidence: 99%

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Antigenic structure of poliovirus in inactivated vaccines

Ferguson¹,

Wood²,

Minor³

1993

Journal of General Virology

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antigenic structure of poliovirus in inactivated vaccines

Ferguson¹,

Wood²,

Minor³

1993

Journal of General Virology

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“…Single radial immunodiffusion antigen blocking tests were performed as described (Ferguson et al, 1984).…”

Section: Methodsmentioning

confidence: 99%

Antigenic structure of chimeras of type 1 and type 3 polioviruses involving antigenic sites 2, 3 and 4

Minor

Ferguson

Katrak

et al. 1991

Journal of General Virology

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“…Antigenic characteristics of the strains were assessed using a microneutralization assay as described by Huovilainen et al (1987), and a panel of type 3-specific MAbs (Ferguson et at., 1984;Minor et al, 1985). Four of the MAbs were directed against site 1 (MAbs 175,204,472 and 495), three were against site 2 (MAbs 882, 875 and 838) and one was against site 3 (879).…”

Section: Methodsmentioning

confidence: 99%

Antigenic Variation among 173 Strains of Type 3 Poliovirus Isolated in Finland during the 1984 to 1985 Outbreak

Huovilainen

Kinnunen

Ferguson

et al. 1988

Journal of General Virology

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SUMMARY Antigenic properties of 128 clinical type 3 poliovirus isolates of the 1984 to 1985Finland outbreak from 95 persons and 45 strains from sewage water specimens were evaluated using five neutralizing monoclonal antibodies (MAbs) directed against an antigenic site (designated site 1) on VP1 at amino acids 89 to 100. All five MAbs neutralized the type 3 poliovirus strains used in the vaccines, P3/Saukett and P3/Sabin, but none of them neutralized the prototype strain of the outbreak (P3/Finland/ 23127/84). Forty-six percent of the clinical isolates resembled the prototype strain (class A) while the rest of the isolates were neutralized by one or more of the MAbs (classes B to D). Although an antigenic drift from A to one of the other classes was observed in sequential specimens from several individuals, no clear-cut overall change in the class distribution was found within the 3 months time span of the outbreak. Homogeneous virus populations were isolated from the sewage specimens using a microtitre endpoint dilution method. The last positive sewage specimens which were obtained in January to February 1985 still had a majority of the class A strain. Some of the clinical isolates were also tested using MAbs directed against distinct antigenic sites. These studies showed that strains that gave the same pattern of reactivity with site 1 MAbs could be differentiated using antibodies directed against other sites. Fifteen strains belonging to different antigenic subclasses were subjected to partial RNA sequencing of the genome region coding for antigenic site t. The antigenic variation was usually, but not always associated with corresponding amino acid substitutions in antigenic site 1. These results indicate that the antigenic sites of type 3 poliovirus vary extensively within a given outbreak and even during replication in a given host. This variation may have both pathogenetic and epidemiological significance. INTRODUCTIONThere are three distinct serotypes of poliovirus that can be distinguished from each other in neutralization tests using polyclonal antisera. Individual clinical poliovirus isolates can be easily classified into one of the three serotypes using unabsorbed antisera. The poliovirus strains used in vaccines were isolated several decades ago but still appear to induce antibodies that protect well against the poliovirus strains that are prevalent today. This would suggest that the antigenic structure of polioviruses is relatively stable. In contrast, it has been shown by oligonucleotide fingerprinting (Nottay et al., 1981 ;Minor et al., 1982) that the genome of polioviruses (like that of other RNA viruses) can vary remarkably during replication in vivo.At least three separate antigenic sites are known to be involved in neutralization of polioviruses. Regions of the three major structural proteins (VP1, 2 and 3) have been identified as antigenic sites and designated sites 1, 2 and 3 (Minor et al., 1985). These sites have also been

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Neutralization Epitopes on Poliovirus Type 3 Particles: an Analysis Using Monoclonal Antibodies

Cited by 46 publications

References 14 publications

Antigenic structure of poliovirus in inactivated vaccines

Antigenic structure of poliovirus in inactivated vaccines

Antigenic structure of chimeras of type 1 and type 3 polioviruses involving antigenic sites 2, 3 and 4

Antigenic Variation among 173 Strains of Type 3 Poliovirus Isolated in Finland during the 1984 to 1985 Outbreak

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