Neutrophils at work

Nauseef, William M.; Borregaard, Niels

doi:10.1038/ni.2921

Cited by 775 publications

(759 citation statements)

References 161 publications

Supporting

Mentioning

740

Contrasting

Unclassified

Order By: Relevance

“…111,112 The endothelium system is critical player of innate defense via releasing inflammatory cytokines and chemokines in the first barrier to recruit monocytes, neutrophils, eosinophils and basophils from the circulation. [113][114][115][116] TLR stimulation induce phenotypic and functional maturation of DC into potent antigen-presenting cells which efficiently initiate and control adaptive immune responses, while in combination with IFN-γ could induce M1 macrophage polarization that is critical for phagocytosis and killing of invading pathogens. In this section, we focus on two important cell populations in innate immunity, DCs as the key bridge between innate and adaptive immunity, and innate lymphoid cells (ILCs) as the key mediator of effector responses during innate immunity.…”

Section: Cellular Regulation Of Innate Immunity and Inflammationmentioning

confidence: 99%

Cellular and molecular regulation of innate inflammatory responses

2016

View full text Add to dashboard Cite

Section: Cellular Regulation Of Innate Immunity and Inflammationmentioning

confidence: 99%

Cellular and molecular regulation of innate inflammatory responses

2016

View full text Add to dashboard Cite

“…Estas células se originam na medula óssea e, quando totalmente diferenciadas e maduras, são liberadas na corrente sanguínea, onde constituem de 50 a 70% do total de leucócitos circulantes e possuem um tempo de vida médio de 8 a 12 horas (Mantovani et al, 2011;Kolaczkowska;Kubes, 2013;Borregaard, 2014).…”

Section: Aspectos Geraisunclassified

“…Para atuar na defesa do organismo, os neutrófilos possuem como principais atividades funcionais: a migração orientada para o local da infecção (quimiotaxia), a aderência ao patógeno e seu englobamento (fagocitose), e a destruição dos microrganismos invasores, através da liberação das substâncias tóxicas presentes nos grânulos e da produção de espécies reativas de oxigênio (ERO) (Kolaczkowska;Kubes, 2013 (Kumar;Sharma, 2010;Kolaczkowska;Kubes, 2013;Borregaard, 2014).…”

Section: Aspectos Geraisunclassified

“…Para atingir os sítios de inflamação ou injúria, os neutrófilos circulantes devem deixar a corrente sanguínea através do endotélio e migrar, ao longo de um gradiente químico, para o local da lesão nos tecidos, processo este chamado de quimiotaxia (Zachariae, 1993 (Amulic et al, 2012;Kolaczkowska;Kubes, 2013;Borregaard, 2014).…”

Section: -Quimiotaxiaunclassified

“…Based on the set of results obtained in this study, we conclude that EEBd has a great potential to become a therapeutic adjuvant in the treatment of neutrophil-mediated inflammatory diseases, and that caffeic acid is a possible chemical marker of the anti-inflammatory activity of the extract. Sharma, 2010;Kolaczkowska;Kubes, 2013;Borregaard, 2014). …”

mentioning

confidence: 99%

See 2 more Smart Citations

O extrato etanólico das folhas de Baccharis dracunculifolia (Asteraceae) modula funções efetoras de neutrófilos: um promissor agente fitoterápico para o tratamento de processos inflamatórios mediados por estas células

Rinhel¹

View full text Add to dashboard Cite

RibeirãoNeutrophils are phagocytic immune cells mobilized primarily to fight against infections. These cells are capable to kill microorganisms through degranulation, which leads to the release of antimicrobial molecules, and production of reactive oxygen species. Although neutrophil recruitment is essential for host protection, excessive activation of these cells culminates in intense discharge of cytotoxic molecules to the extracellular milieu, which can elicit the development of and/or aggravate the clinical condition in autoimmune and inflammatory diseases such as rheumatoid arthritis. Modulation of the effector functions of neutrophils is a possible therapeutic approach to treat these diseases. Previous studies have shown that the ethanolic extract of Baccharis dracunculifolia leaves (EEBd) has a great therapeutic potential due to its significant antioxidant effect towards the oxidative metabolism of neutrophils. To continue investigating the anti-inflammatory and/or immunomodulatory action of such extract, the first part of the present work examined its modulator effect on three important effector functions of human neutrophils that mediate recognition and clearance of pathogens -phagocytosis, degranulation, and microbial killing -and on the activity of lysosomal enzymes. EEBd inhibited the oxidative metabolism of neutrophils without affecting their defense ability, depending on the extract concentration used. To propose possible chemical markers for the biological effects of the extract, the second part of this study evaluated the antioxidant activity of seven compounds isolated from EEBd -caffeic acid, coumaric acid, ferulic acid, cinnamic acid, aromadendrin-4'-methyl ether, isosakuranetin, and hispidulin -towards the oxidative metabolism of neutrophils. The results pointed to caffeic acid as a potential chemical marker for this biological activity. The third part of this investigation aimed to improve the bioavailability and favor the therapeutic use of the natural products studied. EEBd and caffeic acid were incorporated into liposomal carrier systems. The incorporation of both samples into liposomes did not alter their antioxidant activity towards neutrophils, as compared with their respective free forms. Finally, this study examined the therapeutic effect of EEBd and caffeic acid, both in the free form and incorporated into liposomes, in an animal model of arthritis. The free samples markedly improved the joint inflammatory parameters: they reduced edema and migration of total cells and neutrophils, and slightly lowered the levels of the inflammatory cytokines tumor necrosis factor  and interleukin 6 and 1 (TNF-, IL-6, and IL-1, respectively). In addition, incorporation of EEBd and caffeic acid into liposomes can help to reduce their concentrations required to achieve the desired therapeutic effect. Based on the set of results obtained in this study, we conclude that EEBd has a great potential to become a therapeutic adjuvant in the treatment of neutrophil-mediated inflammatory diseases, and that...

show abstract

Resolvin E1, but not resolvins E2 and E3, promotes fMLF‐induced ROS generation in human neutrophils

et al. 2018

View full text Add to dashboard Cite

E-series resolvins are biosynthesized from eicosapentaenoic acid during the resolution phase of acute inflammation and enhance inflammation resolution. However, the role of E-series resolvins in inflammation resolution is not yet known. Herein, we show that in human polymorphonuclear neutrophils (PMNs), resolvin E1 (RvE1) selectively enhances reactive oxygen species (ROS) generation induced by N-formylmethionyl-leucyl-phenylalanine. The RvE1-mediated enhancement is eliminated by a pan-antagonist of leukotriene B4 (LTB4) receptors, LY255283, or an NADPH oxidase inhibitor, diphenyleneiodonium. Thus, RvE1 enhances NADPH oxidase-mediated ROS generation via LTB4 receptors. Unlike RvE1, resolvins E2 and E3 do not show such activation of PMNs. Our findings suggest that RvE1 contributes to regulation of ROS generation, in accordance with the inflammatory state of the host.

show abstract

Neutrophils at work

Cited by 775 publications

References 161 publications

Cellular and molecular regulation of innate inflammatory responses

Cellular and molecular regulation of innate inflammatory responses

O extrato etanólico das folhas de Baccharis dracunculifolia (Asteraceae) modula funções efetoras de neutrófilos: um promissor agente fitoterápico para o tratamento de processos inflamatórios mediados por estas células

Resolvin E1, but not resolvins E2 and E3, promotes fMLF‐induced ROS generation in human neutrophils

Contact Info

Product

Resources

About