New and Old Genes Associated with Topotecan Resistance Development in Ovarian Cancer Cell Lines

doi:10.21873/anticanres.11493

Cited by 25 publications

(34 citation statements)

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“…Increased expression after a short time of exposure confirms the significance of ABCC2 gene in CIS resistance. Previously, we also observed increased expression of ABCG2 gene, after a short time of exposure to TOP, in ovarian cancer cell lines [ 19 ]. Thus, the first response to the cytotoxic agent seems to indicate the direction of response to the drug.…”

Section: Discussionmentioning

confidence: 64%

“…CIS is the most important drug used in the first line of ovarian cancer chemotherapy [ 3 ], and TOP is used in the second line of ovarian cancer chemotherapy in case of platinum and taxane resistance in patients [ 5 ]. Previously, we described the expression of “old” genes related to CIS or TOP resistance in investigated cell lines [ 17 , 18 , 19 , 20 ]. Recently, we detected the expression of some “new” genes that may be related to drug resistance in ovarian cancer [ 19 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

“…Previously, we described the expression of “old” genes related to CIS or TOP resistance in investigated cell lines [ 17 , 18 , 19 , 20 ]. Recently, we detected the expression of some “new” genes that may be related to drug resistance in ovarian cancer [ 19 , 25 ]. Here, we describe the expression of four new genes in established and primary responses to CIS and TOP treatment.…”

Section: Discussionmentioning

confidence: 99%

“…The most significant mechanism of resistance to this drug is active transport from cancer cells. The most important protein playing a role in this phenomenon is BCRP (breast cancer resistant protein) encoded by the ABCG2 gene [ 16 , 17 , 18 , 19 ]. Previously, we reported that the expression of the ABCB1 ( MDR1 ) gene, encoding the P-gp protein, may also be related to TOP resistance [ 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…The most important protein playing a role in this phenomenon is BCRP (breast cancer resistant protein) encoded by the ABCG2 gene [ 16 , 17 , 18 , 19 ]. Previously, we reported that the expression of the ABCB1 ( MDR1 ) gene, encoding the P-gp protein, may also be related to TOP resistance [ 18 , 19 , 20 ]. Another important mechanism of TOP resistance involves mutations in DNA topoisomerases or decreased expression of these enzymes, making them less sensitive to drugs [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

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New and Old Genes Associated with Primary and Established Responses to Cisplatin and Topotecan Treatment in Ovarian Cancer Cell Lines

et al. 2017

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Low efficiency of chemotherapy in ovarian cancer results from the development of drug resistance. Cisplatin (CIS) and topotecan (TOP) are drugs used in chemotherapy of this cancer. We analyzed the development of CIS and TOP resistance in ovarian cancer cell lines. Incubation of drug sensitive cell lines (W1 and A2780) with cytostatic drugs was used to determine the primary response to CIS and TOP. Quantitative polymerase chain reaction (Q-PCR) was performed to measure the expression levels of the genes. We observed decreased expression of the MCTP1 gene in all resistant cell lines. We observed overexpression of the S100A3 and HERC5 genes in TOP-resistant cell lines. Increased expression of the S100A3 gene was also observed in CIS-resistant A2780 sublines. Overexpression of the C4orf18 gene was observed in CIS- and TOP-resistant A2780 sublines. A short time of exposure to CIS led to increased expression of the ABCC2 gene in the W1 and A2780 cell lines and increased expression of the C4orf18 gene in the A2780 cell line. A short time of exposure to TOP led to increased expression of the S100A3 and HERC5 genes in both sensitive cell lines, increased expression of the C4orf18 gene in the A2780 cell line and downregulation of the MCTP1 gene in the W1 cell line. Our results suggest that changes in expression of the MCTP1, S100A3 and C4orf18 genes may be related to both CIS and TOP resistance. Increased expression of the HERC5 gene seems to be important only in TOP resistance.

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Section: Discussionmentioning

confidence: 64%