New and potent production platform of the acetylcholinesterase inhibitor huperzine A by gamma-irradiated Alternaria brassicae under solid-state fermentation

Zaki, Amira G; El‐Sayed, El‐Sayed R.

doi:10.1007/s00253-021-11678-0

Cited by 16 publications

(6 citation statements)

References 42 publications

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“…RSM is a powerful approach for optimizing various applications such as composition of media, bioprocess improvement, physical and chemical factors affecting microbial growth, and enzyme and protein yield 28 . Media optimization is achieved by monitoring the impact of the components/nutrients on the microbial development using fermentation protocols, choosing critical parameters and optimizing them 29 . RSM exposes the interaction between process variables on a targeted response, and assists us to attain a general optimization with decreased experimental trials compared to conventional one factor at a time method, which consequently saves resources, time, and effort 30 .…”

Section: Discussionmentioning

confidence: 99%

Unlocking the biosynthetic potential of Penicillium roqueforti for hyperproduction of the immunosuppressant mycophenolic acid: Gamma radiation mutagenesis and response surface optimization of fermentation medium

El‐Sayed

Zaki

2022

Biotech and App Biochem

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Based on the broad clinical utility of the immunosuppressant mycophenolic acid (MPA), this article aims to intensify the biosynthetic potential of Penicillium roqueforti for more effective hyperproduction of the drug. Several mutants were generated from irradiation mutagenesis and screened. Two strains (GM1013 and GM1093) presented an elevated MPA productivity with significant yield constancy over 10 subsequent generations. By investigating the effect of some phosphorous sources and mineral salts on MPA production by the two mutants, KH 2 PO 4 and FeSO 4 ⋅7H 2 O were most preferred by the two mutants for higher MPA production rates. Statistics-dependent experimental designs were also employed for optimizing medium components for maximum MPA production.Medium components were primarily screened using the Plackett-Burman model to demonstrate the most important components that most significantly affect MPA production. The concentrations of these significant components were then optimized through a central composite rotatable model. In conclusion, gammaradiation mutation and response surface optimization resulted in a promising MPA productivity by P. roqueforti GM1013. To our knowledge, the MPA-yield achieved in this study (2933.32 mg L -1 ) is the highest reported by academic laboratories from P. roqueforti cultures, which could be of economic value for a prospective large industrialized application.

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Section: Discussionmentioning

confidence: 99%

Unlocking the biosynthetic potential of Penicillium roqueforti for hyperproduction of the immunosuppressant mycophenolic acid: Gamma radiation mutagenesis and response surface optimization of fermentation medium

El‐Sayed

Zaki

2022

Biotech and App Biochem

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“…Moreover, the same effect of exposure to gamma rays on the survival and the fungal growth was observed by previous reports (Awan et al 2011 ; El-Sayed and Zaki 2022 ; El-Sayed et al 2019a ; b ; c ). Previous reports concluded the effect of gamma rays as an energetic ionizing radiation in induction of mutations in the exposed cells (Thacker 1999 ) through the repair mechanism in genes of the DNA of the irradiated cells (Ismaiel et al 2014 , 2015 ; Zaki et al 2020 ; Zaki and El-Sayed 2021 ). Generally, exposure to physical mutagens such as gamma rays is a successful strategy to improve microbial strains by developing overproducers (Parekh et al 2000 ) with intensified production rates (El-Sayed et al 2020a , c ; Zaki et al 2020 ) with industrial applications thereby effectively lowering the overall cost of the process, as supported by several reports (Anwar et al 2022 ; El-Sayed et al 2020d , e , f ; El-Sayed 2021 ; El-Sayed et al 2022b ; c ).…”

Section: Discussionmentioning

confidence: 99%

Bioprospecting endophytic fungi for antifeedants and larvicides and their enhancement by gamma irradiation

et al. 2022

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The search and discovery of new natural products with antifeedant and larvicidal potentials to mitigate harmful insects are scientific pressing issues in the modern agriculture. In this paper, the antifeedant and larvicidal potentials of 69 fungal isolates were screened against the Egyptian cotton leafworm Spodoptera littoralis. A total of 17 isolates showed the insecticidal potentials with three promising isolates. These strains were Aspergillus sydowii, Lasiodiplodia theobromae, and Aspergillus flavus isolated from Ricinus communis (bark), Terminalia arjuna (Bark), and Psidium guajava (twigs), respectively. The effect of gamma irradiation on the antifeedant and larvicidal activities of the three strains was investigated. Exposure of the fungal spores to 1000 Gy of gamma rays significantly intensified both the antifeedant and larvicidal potentials. To identify compounds responsible for these activities, extracts of the three strains were fractionated by thin layer chromatography. The nature of the separated compounds namely, Penitrem A, 1, 3, 5, 8- tetramethyl- 4, 6-diethyl- 7- [2- (methoxycarbonyl)ethyl] porphyrin (from A. sydowii), Penitrem A, 2, 7, 12, 17-Tetramethyl-3, 5:8, 10:13, 15:18, 20-tetrakis (2,2-dimethylpropano) porphyrin (from A. flavus), N,N-Diethyl-3-nitrobenzamide, and Diisooctyl-phthalate (from L. theobromae) were studied by GC-MS analysis. These findings recommend endophytic fungi as promising sources of novel natural compounds to mitigate harmful insects.

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“…Statistical optimization approaches like response surface methodology (RSM) are commonly used to investigate responses after simultaneous variation of multiple process parameters (Tortella et al 2019 ; Zaki et al 2019 ; Saeed et al 2020 ; Zaki and El-Sayed 2021 ). By employing the response surface methodology, the nanocomposite fabrication showed a better response to variations in the different investigated parameters as pH, reaction time, AgNO 3 concentration and RH concentration.…”

Section: Discussionmentioning

confidence: 99%

Novel fabrication of SiO2/Ag nanocomposite by gamma irradiated Fusarium oxysporum to combat Ralstonia solanacearum

2022

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The bacterial wilt is a global destructive plant disease that initiated by the phytopathogenic Ralstonia solanacearum. This study display a novel biofabrication of silica/silver nanocomposite using Fusarium oxysporum-fermented rice husk (RH) under solid state fermentation (SSF). The biofabricated nanocomposite was characterized by XRD, UV–Vis. spectroscopy, DLS, SEM, EDX elemental mapping, and TEM analyses as well as investigated for anti-R. solanacearum activity. Response surface methodology was also processed for optimizing the biofabrication process and improving the anti-bacterial activity of the fabricated nanocomposite. Maximum suppression zone of 29.5 mm against R. solanacearum was reached at optimum RH content of 6.0 g, AgNO3 concentration of 2.50 mM, reaction pH of 6.3, and reaction time of 2 days. The anti-R. solanacearum activity of the fabricated nanocomposite was further improved by exposing the F. oxysporum strain to a gamma irradiation dose of 200 Gy. In conclusion, RH recycling under SSF by F. oxysporum could provide an innovative, facile, non-expensive, and green approach for fabricating SiO2/Ag nanocomposite that could be applied efficiently as an eco-friendly antibacterial agent to combat R. solanacearum in agricultural applications. Moreover, the developed method could serve as a significant platform for the designing of new nanostructures for broad applications.

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New and potent production platform of the acetylcholinesterase inhibitor huperzine A by gamma-irradiated Alternaria brassicae under solid-state fermentation

Cited by 16 publications

References 42 publications

Unlocking the biosynthetic potential of Penicillium roqueforti for hyperproduction of the immunosuppressant mycophenolic acid: Gamma radiation mutagenesis and response surface optimization of fermentation medium

Unlocking the biosynthetic potential of Penicillium roqueforti for hyperproduction of the immunosuppressant mycophenolic acid: Gamma radiation mutagenesis and response surface optimization of fermentation medium

Bioprospecting endophytic fungi for antifeedants and larvicides and their enhancement by gamma irradiation

Novel fabrication of SiO2/Ag nanocomposite by gamma irradiated Fusarium oxysporum to combat Ralstonia solanacearum

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