New approach for simultaneous analysis of commonly used antigout drugs by HPLC/UV method; Application in pharmaceutical and biological analysis

Abstract

An innovative, simple, fast, specific and sensitive high performance liquid chromatographic method with Ultraviolet detection (HPLC/UV) was established and validated for simultaneous determination of a combination of three commonly prescribed antigout drugs namely; Colchicine (CLN), Probenecid (PRD) and Febuxostat (FBX) in dosage forms and in human urine samples. GraceSmart RP C18 column was utilized for separation of the studied mixture using isocratic mode of mobile system consisting of acetonitrile: water (55: 45, v/v; containing 0.5% v/v formic acid). Flow rate and detection wavelengths programming were designed to obtain rapid and efficient separation. The cited drugs were separated within less than 9 minutes. The retention times (tR) were 3.597, 5.357 and 8.250 for CLN, PRD and FBX; respectively. The linearity range for all the investigated drugs was 0.040 -50.0 µg/mL with detection limits of 1.94 -6.48 ng/mL. Additionally, the developed method was validated according to ICH and US-FDA guidelines and was successfully applied for simultaneous estimation of the studied drugs in their pure form, and in laboratory-made tablets mixture. The proposed method was also used for analysis of CLN and PRD in their combined tablets and in human urine samples obtained from healthy volunteers with good recoveries (98.39-101.87%). Furthermore, the stability of CLN-PRD mixture in urine samples was studied. This method is suitable for quality control purposes for simultaneous analysis of these co-administered antigout agents in their binary and ternary mixtures.

Novel Heterogeneous Fenton’s-Like Catalysis for Degradation of Colchicine Coupled with Extraction of Its Biologically Active Metabolite

“…The lower LOD and LOQ values obtained (16.4 and 49.8 pg mL -1 ; respectively) indicate a high sensitivity of the proposed method. In conclusion, the method's simplicity and sensitivity are superior to other reported LC/MS or Tandem MS methods[23][24][25][26][27][28].3.1.2.2. Accuracy and precisionLow, medium, and high concentration ranges (0.100, 20.0, and 50.0 ng mL -1 ) of CLN were analyzed by the developed LC/MS method.…”

Drug Analysis Methods

“…Традиционные стандартные методы оценки качества лекарственных препаратов включают спектрофотометрию в УФ, видимой и средней ИК-области, флуоресцентные методы (в сочетании с модификациями квантовыми точками для визуализации) [2][3][4][5], спектроскопию ядерного магнитного резонанса [5] высокоэффективную жидкостную хроматографию (ВЭЖХ, HPLC) [6][7][8][9], газовую хроматографию [10], иммуноанализ (анализ с применением соответствующих антител) [11], различные модификации масс-спектрометрических методов [12][13][14], а также комбинации методов, такие как HPLC/UV детектирование [15], HPLC-MS/MS анализ [16].…”

“…ВЭЖХ за последние десятилетия во многом определила заметный прогресс в фармации и практической медицине. ВЭЖХ как универсальный и высокочувствительный метод анализа позволяет одновременно осуществлять контроль за содержанием нескольких лекарственных веществ, вспомогательных компонентов и возможных токсичных примесей в многокомпонентных системах, отличаясь при этом высокой точностью и воспроизводимостью [6][7][8][9].…”

Method Development and Optimization for Colchicine Estimation in Toxicological Analysis using Dispersive LiquidLiquid Micro-Extraction and HPLC-UV

“…The toxicological analysis of colchicine with optimized extraction and efficient instrumentation is hence necessary for the field of forensic science and medicine. RP-HPLC has proven to be one of the best instruments for the analysis of colchicine along with the routine liquid extraction (21)(22)(23) . The field of analytical extractions has improved throughout the years and microextraction is considered to be one of the best sample preparation techniques employed for alkaloids because of its efficiency (24) .…”