New approach to improve encapsulation and antitumor activity of doxorubicin loaded in solid lipid nanoparticles

Mussi, Samuel Vidal; Silva, Renata Carvalho; Oliveira, Mônica Cristina; Lucci, Carolina Madeira; Azevedo, Ricardo Bentes; Ferreira, Lucas Antônio Miranda

doi:10.1016/j.ejps.2012.10.025

Cited by 101 publications

(65 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Notably, SLN's have been investigated for oral drug delivery and have been shown to exhibit good release characteristics and systemic bioavailability of the encapsulated drugs (Demirel et al 2001;Yang, et al 1999). SLN tolerability and toxicity has also been characterised in vivo (Fundarò et al 2000;Sanna et al 2004) as well as in vitro (Müller et al 1997;Mussi, et al 2013;Nassimi, et al 2010), and they are regarded to be generally well tolerated, owing to their composition from physiologically similar lipids.…”

Section: Introductionmentioning

confidence: 99%

A novel approach to oral iron delivery using ferrous sulphate loaded solid lipid nanoparticles

Zariwala

El-Said

Jackson

et al. 2013

International Journal of Pharmaceutics

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

A novel approach to oral iron delivery using ferrous sulphate loaded solid lipid nanoparticles

Zariwala

El-Said

Jackson

et al. 2013

International Journal of Pharmaceutics

View full text Add to dashboard Cite

“…27,28 Stahl et al have presented two criteria that determine ion pairing to create insoluble salt forms. First, the difference in pKa between the acid and the base must be at least 2 pH units to ensure sufficient proton transfer.…”

mentioning

confidence: 99%

Alendronate-coated long-circulating liposomes containing 99mtechnetium-ceftizoxime used to identify osteomyelitis

Ferreira¹,

Boratto²,

Cardoso³

et al. 2015

IJN

View full text Add to dashboard Cite

Osteomyelitis is a progressive destruction of bones caused by microorganisms. Inadequate or absent treatment increases the risk of bone growth inhibition, fractures, and sepsis. Among the diagnostic techniques, functional images are the most sensitive in detecting osteomyelitis in its early stages. However, these techniques do not have adequate specificity. By contrast, radiolabeled antibiotics could improve selectivity, since they are specifically recognized by the bacteria. The incorporation of these radiopharmaceuticals in drug-delivery systems with high affinity for bones could improve the overall uptake. In this work, long-circulating and alendronate-coated liposomes containing 99m technetium-radiolabeled ceftizoxime were prepared and their ability to identify infectious foci (osteomyelitis) in animal models was evaluated. The effect of the presence of PEGylated lipids and surface-attached alendronate was evaluated. The bone-targeted long-circulating liposomal 99m technetium–ceftizoxime showed higher uptake in regions of septic inflammation than did the non-long-circulating and/or alendronate-non-coated liposomes, showing that both the presence of PEGylated lipids and alendronate coating are important to optimize the bone targeting. Scintigraphic images of septic or aseptic inflammation-bearing Wistar rats, as well as healthy rats, were acquired at different time intervals after the intravenous administration of these liposomes. The target-to-non-target ratio proved to be significantly higher in the osteomyelitis-bearing animals for all investigated time intervals. Biodistribution studies were also performed after the intravenous administration of the formulation in osteomyelitis-bearing animals. A significant amount of liposomes were taken up by the organs of the mononuclear phagocyte system (liver and spleen). Intense renal excretion was also observed during the entire experiment period. Moreover, the liposome uptake by the infectious focus was significantly high. These results show that long-circulating and alendronate-coated liposomes containing 99m technetium-radiolabeled ceftizoxime have a tropism for infectious foci.

show abstract

“…Notably, in all cell lines, VRS-NLCs showed better performance than free VRS at most concentrations. The lipophilicity of NLCs might increase the interaction between particles with the membranes of cells resulting in high internalization into cytosol (Mussi et al, 2013). In addition, slow release profile and rapid penetration provided safety in blood circulation as well as efficacy in killing the cancer cell.…”

Section: Cellular Uptake and In Vitro Cytotoxicitymentioning

confidence: 99%

Development of lipid nanoparticles for a histone deacetylases inhibitor as a promising anticancer therapeutic

et al. 2014

View full text Add to dashboard Cite

Background: Vorinostat (VRS), a histone deacetylases inhibitor, has significant cytotoxic potential in a large number of human cancer cell lines. Objective: To clarify its promising anticancer potential and to improve its drawback related to physical properties and in vivo performance of VRS. Methods: VRS was successfully incorporated into nanostructured lipid carriers (NLCs) by the hot microemulsion method using sonication following a homogenization technique. Results: After the optimization process, VRS-loaded NLCs (VRS-NLCs) were obtained as ideal quality nanoparticles with a spherical shape, small size ($150 nm), negative charge ($À22 mV), and narrow size distribution. In addition, the high entrapment efficiency ($99%) and sustained drug release profile were recorded. Cytotoxicity study in three different cell lines (A549, MCF-7, and SCC-7) demonstrated higher cytotoxicity of VRS-NLCs than free drug. Finally, the AUC of VRS (118.16 ± 17.35 mgh/mL) was enhanced $4.4 times compared with that of free drug (27.03 ± 3.25 mgh/mL). Conclusion: These results suggest the potential of NLCs as an oral delivery system for enhancement of cellular uptake, in vitro cytotoxicity in cancer cell lines and the oral bioavailability of VRS.

show abstract

New approach to improve encapsulation and antitumor activity of doxorubicin loaded in solid lipid nanoparticles

Cited by 101 publications

References 35 publications

A novel approach to oral iron delivery using ferrous sulphate loaded solid lipid nanoparticles

A novel approach to oral iron delivery using ferrous sulphate loaded solid lipid nanoparticles

Alendronate-coated long-circulating liposomes containing 99mtechnetium-ceftizoxime used to identify osteomyelitis

Development of lipid nanoparticles for a histone deacetylases inhibitor as a promising anticancer therapeutic

Contact Info

Product

Resources

About