New approaches in sensitive chiral CE

Abstract

CE has shown to have a big potential for chiral separations, with advantages such as high efficiency, high resolution, and low sample and reagents consumption. Nevertheless, when UV detection is employed, CE has some drawbacks, especially the low sensitivity obtained due to the short optical path length. Notwithstanding, sensitivity improvements can be achieved when different approaches are employed, such as sample treatment strategies (off-line or on-line), in-capillary sample preconcentration techniques, and/or alternative detection systems to UV-Vis (such as fluorescence, conductimetry, electrochemiluminiscence, MS, etc.). This article reviews the most recent methodological and instrumental advances reported from June 2011 to May 2013 for enhancing the sensitivity in chiral analysis by CE. The sensitivity achieved for the enantioseparated analytes and the applications carried out using the developed methodologies are also summarized.

Improving the sensitivity in chiral capillary electrophoresis

“…In order to extract or isolate a compound from the matrix components, the use of an extraction technique is necessary. Liquid–liquid extraction (LLE) and SPE are the most known extraction techniques; however, as anticipated in the previous review article regarding this topic , SPE is less used every time and as a consequence, regarding off‐line sample treatments, only LLE has been used to enhance the sensitivity in chiral CE methodologies in the period of time covered by this review.…”

Chiral separation of d/l-aldoses by micellar electrokinetic chromatography using a chiral derivatization reagent and a phenylboronic acid complex

“…It is important in various chemical fields, especially in the pharmaceutical industry, food production, clinical toxicology, and forensic toxicology [1][2][3][4][5][6]. As a consequence, the development of effective methods for chiral separation is a significant discipline in the analytical sciences [7]. Direct chiral separations are often achieved in the presence of a chiral selector that is, in most cases, bound to a solid support in high-performance liquid chromatography, whereas it is added to the background electrolyte (BGE) in capillary electrophoresis (CE).…”

Method for determination of optical purity of 2‐arylpropanoic acids using urea derivatives based on a 1,1′‐binaphthalene skeleton as chiral NMR solvating agents: Advantages and limitations thereof

“…For example, high-performance liquid chromatography (HPLC), [15][16][17][18][19][20][21] gas chromatography (GC), 22 capillary electrophoresis (CE), 23 or nuclear magnetic resonance (NMR) 24 can be employed. For example, high-performance liquid chromatography (HPLC), [15][16][17][18][19][20][21] gas chromatography (GC), 22 capillary electrophoresis (CE), 23 or nuclear magnetic resonance (NMR) 24 can be employed.…”