New approaches to single-cell analysis by capillary electrophoresis

Zabzdyr, Jennifer L.; Lillard, Sheri J.

doi:10.1016/s0165-9936(01)00051-6

Cited by 32 publications

(19 citation statements)

References 35 publications

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“…detection and isolation of rare stem cells and circulating tumor cells [74–75] and cellular metabolism [76–77]) grow, a cell sorter capable of sorting single cells with high throughput, purity, and cell viability has become indispensable for a microfabricated flow cytometer. The cell sorter typically operates under continuous flow separation [22].…”

Section: Cell Sorting Techniquesmentioning

confidence: 99%

Microfluidics and photonics for Bio‐System‐on‐a‐Chip: A review of advancements in technology towards a microfluidic flow cytometry chip

Godin

Chen

Cho

et al. 2008

Journal of Biophotonics

150

106

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Microfluidics and photonics come together to form a field commonly referred to as ‘optofluidics’. Flow cytometry provides the field with a technology base from which both microfluidic and photonic components be developed and integrated into a useful device. This article reviews some of the more recent developments to familiarize a reader with the current state of the technologies and also highlights the requirements of the device and how researchers are working to meet these needs. A microfluidic flow cytometer protoype employing on-chip lenses for illumination and light collection in conjunction with a microfluidic sample flow system for device miniaturization.

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Section: Cell Sorting Techniquesmentioning

confidence: 99%

Microfluidics and photonics for Bio‐System‐on‐a‐Chip: A review of advancements in technology towards a microfluidic flow cytometry chip

Godin

Chen

Cho

et al. 2008

Journal of Biophotonics

150

106

View full text Add to dashboard Cite

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“…CE is a technique that has evolved significantly due to the use of microtechnology [43]. The capillary structures are relatively simple to make in several materials, the devices are suitable for coupling to sensitive detection methods, and they are cheap to produce [43][44][45][46].…”

Section: Overview Of Existing Designsmentioning

confidence: 99%

“…The capillary structures are relatively simple to make in several materials, the devices are suitable for coupling to sensitive detection methods, and they are cheap to produce [43][44][45][46]. In conventional CE of cell content, a batch of cells is lysed after which a small sample is analyzed [47].…”

Section: Overview Of Existing Designsmentioning

confidence: 99%

Electroporation of cells in microfluidic devices: a review

et al. 2006

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In recent years, several publications on microfluidic devices have focused on the process of electroporation, which results in the poration of the biological cell membrane. The devices involved are designed for cell analysis, transfection or pasteurization. The high electric field strengths needed are induced by placing the electrodes in close proximity or by creating a constriction between the electrodes, which focuses the electric field. Detection is usually achieved through fluorescent labeling or by measuring impedance. So far, most of these devices have only concerned themselves solely with the electroporation process, but integration with separation and detection processes is expected in the near future. In particular, single-cell content analysis is expected to add further value to the concept of the microfluidic chip. Furthermore, if advanced pulse schemes are employed, such microdevices can also enhance research into intracellular electroporation.

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“…From the mid-1900s, scientists and engineers started to have strong interest in manipulation biological particles techniques, not just in observation of stationary microscopic particles [1]. In order to meet the demands of observation and analysis of cellular and intracellular components, much research have been done to find an effectively way to manipulate cells at single-cell-level by researchers [2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Single Cell Manipulation Technology

Lin¹,

Chen²,

Xie³

et al. 2015

Nano BioMed ENG

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With microfluidic technique emerging, cell manipulation technology combines with microfluidic become a promising tool for single-cell-level manipulation. To obtain a kind of or single pure target cell for eliminating interference of useless cells in the trial of molecular biology, genetic analysis, proteomics and single cell analysis, various cell manipulation techniques have been developed for recovery specific cells. In this review, we introduce the principles of each cell manipulation technology and overlook the latest achievements of cell manipulation technique by categorizing externally applied manipulation forces: optical, electrical, magnetic, acoustic, mechanical. We also summarize the advantages and drawbacks of each cell manipulation technique.

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New approaches to single-cell analysis by capillary electrophoresis

Cited by 32 publications

References 35 publications

Microfluidics and photonics for Bio‐System‐on‐a‐Chip: A review of advancements in technology towards a microfluidic flow cytometry chip

Microfluidics and photonics for Bio‐System‐on‐a‐Chip: A review of advancements in technology towards a microfluidic flow cytometry chip

Electroporation of cells in microfluidic devices: a review

Single Cell Manipulation Technology

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