New Bacterial Compositions in Root-filled Teeth with Periradicular Lesions

Schirrmeister, Jörg F.; Liebenow, Anna-Lisa; Pelz, Klaus; Wittmer, Annette; Serr, Annerose; Hellwig, Elmar; Al‐Ahmad, Ali

doi:10.1016/j.joen.2008.10.024

Cited by 100 publications

(108 citation statements)

References 39 publications

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“…S. mutans was chosen since it is considered to be a principal causative agent of dental caries (Beighton et al, 1991;Al-Ahmad et al, 2006;Yoo et al, 2011). E. faecalis is frequently isolated in secondary endodontic infections and is considered to be capable of withstanding unfavourable environmental conditions in obturated root canals (Schirrmeister et al, 2009;Zhu et al, 2010). Apart from testing the effects of APDT on planktonic bacteria it is necessary to study the effectiveness of new antimicrobial therapeutic modalities against adherent bacteria in biofilms, due to the higher antibiotic resistance of micro-organisms embedded within biofilms (Costerton et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial photodynamic therapy using visible light plus water-filtered infrared-A (wIRA)

Al‐Ahmad

Tennert

Karygianni

et al. 2013

Journal of Medical Microbiology

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The aim of this study was to investigate the effectiveness of antimicrobial photodynamic therapy (APDT) using visible light together with water-filtered infrared-A (VIS+wIRA) to eradicate single species of planktonic bacteria and micro-organisms during initial oral bacterial colonization in situ. A broadband VIS+wIRA radiator with a water-filtered spectrum in the range 580-1400 nm was used for irradiation. Toluidine blue (TB) was utilized as a photosensitizer at concentrations of 5, 10, 25 and 50 mg ml "1 . The unweighted (absolute) irradiance was 200 mW cm "2 and it was applied for 1 min. Planktonic cultures of Streptococcus mutans and Enterococcus faecalis were treated with APDT. Salivary bacteria harvested by centrifugation of native human saliva were also tested. In addition, initial bacterial colonization of bovine enamel slabs carried in the mouths of six healthy volunteers was treated in the same way. Up to 2 log 10 of S. mutans and E. faecalis were killed by APDT. Salivary bacteria were eliminated to a higher extent of 3.7-5 log 10 . All TB concentrations tested proved to be highly effective. The killing rate of bacteria in the initial oral bacterial colonization was significant (P50.004) at all tested TB concentrations, despite the interindividual variations found among study participants. This study has shown that APDT in combination with TB and VIS+wIRA is a promising method for killing bacteria during initial oral colonization. Taking the healing effects of wIRA on human tissue into consideration, this technique could be helpful in the treatment of peri-implantitis and periodontitis. INTRODUCTIONAlthough photodynamic therapy (PDT) is currently being applied in cancer cell elimination (Allison & Sibata, 2010;Ericson et al., 2008), more attention has been paid to its application in antimicrobial treatment (Wood et al., 2006;Donnelly et al., 2007;Maisch, 2007). Due to the growing number of antibiotic-resistant micro-organisms, research is focusing on PDT as an alternative chemotherapy modality (Garcez et al., 2010;Kharkwal et al., 2011). Since PDT causes damage to different parts of microbial cells and affects different interaction pathways in the micro-organisms, the development of antibiotic resistance against PDT can be excluded (Wainwright et al., 2010).PDT impresses with its excellent target-cell specificity and high efficiency. The photodynamic effect requires a photosensitizer, suitable light and oxygen. The photosensitizer can be transferred to the high-energy triplet state and reacts with oxygen, producing singlet oxygen and other radical species which themselves cause increased target cell damage (Konopka & Goslinski, 2007). The photosensitizer should be non-toxic and show antimicrobial activity only after activation by illumination. In addition it should not be bio-destructible. Light sources used in PDT should produce low-power visible light at a specific wavelength (or range of wavelengths) which itself does not harm human tissues in the absence of the photosensitizer. Additionally, light s...

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Section: Discussionmentioning

confidence: 99%

Antimicrobial photodynamic therapy using visible light plus water-filtered infrared-A (wIRA)

Al‐Ahmad

Tennert

Karygianni

et al. 2013

Journal of Medical Microbiology

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“…Factors that contribute to a persistent periradicular infection after root canal treatment include Most persistent organism that, despite making up a small proportion of the flora in untreated canals, plays a major role in the aetiology of persistent periradicular lesions after root canal treatment. It is commonly found in a high percentage of root canal failures and it is able to survive in the root canal as a single organism or as a major component of flora (8) .Enterococcus faecalis are gram positive cocci that occur singly, in pairs or in short chains. They are facultative anaerobes, possessing the ability to grow in the presence or absence of oxygen.…”

Section: Introductionmentioning

confidence: 99%

“…It is active against viruses, bacteria, bacterial spores and yeasts (16) via the production of hydroxyl free radicals which attack proteins and DNA. (7) It has been shown that NaOCl, combined with H2O2, is no more effective against E. faecalis than NaOCl alone; (8) MTAD, a common intracanal irrigant, consists of 3% doxycycline, 4.5% citric acid, and 0.5% polysorbate 80 detergent and is used to remove pathogenic bacteria and smear layers during root canal procedures. MTAD has many advantages in root canal irrigation, but its bactericidal activity remains to be improved upon as its antibacterial effect has been largely attributed to doxycycline, a tetracycline that is bacteriostatic rather than bactericidal.…”

Section: Introductionmentioning

confidence: 99%

Comparison of New and Conventional Root Canal Irrigants and their Efficacy on Treatment Outcomes in Endodontics

Soumya¹

2017

JMSCR

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“…Bactérias e seus subprodutos são considerados os principais agentes etiológicos das patologias pulpares e periapicais 1 , e provocam a persistência da infecção, devido à falha do tratamento endodôntico [2][3][4][5][6] e/ou à microinfiltração coronária, que permite a recontaminação microbiana dos canais radiculares 4,5,7 .…”

Section: Introductionunclassified

“…Em casos de retratamento endodôntico, micro-organismos têm sido isolados em 100% dos dentes tratados endodonticamente, após a remoção do material obturador 6 . A microbiota de um dente com insucesso endodôntico e lesão periapical mostra-se diferente daquela encontrada em dentes com necrose pulpar [2][3][4]8 , predominando bactérias anaeróbias facultativas Gram-positivas 6,[8][9][10] .…”

Section: Introductionunclassified

Avaliação da suscetibilidade antimicrobiana de bactérias anaeróbias facultativas isoladas de canais radiculares de dentes com insucesso endodôntico frente aos antibióticos de uso sistêmico

Santi

Barbosa-Ribeiro

Endo

et al. 2015

Rev. odontol. UNESP

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ResumoIntrodução: Bactérias associadas ao insucesso do tratamento endodôntico são capazes de adquirir e expressar resistência aos agentes antimicrobianos comumente empregados para tratar infecções, o que torna necessário, em determinadas situações, a realização de testes laboratoriais para detectar a resistência ou a suscetibilidade antimicrobiana desses micro-organismos. Objetivo: avaliar a suscetibilidade antimicrobiana das cepas de Enterococcus faecalis, Enterococcus faecium, Actinomyces viscosus e Staphylococcus aureus isoladas de canais radiculares de dentes com insucesso endodôntico. Material e método: Cepas clínicas de Enterococcus faecalis (n=3), Enterococcus faecium (n=3), Actinomyces viscosus (n=3) e Staphylococcus aureus (n=3), coletadas in vivo de canais radiculares com insucesso endodôntico, foram testadas quanto à suscetibilidade antimicrobiana por meio do método E-test em duplicata, utilizando os antibióticos: Amoxicilina (AC), Rifampicina (RI), Moxifloxacina (MX), Vancomicina (VA), Tetraciclina (TC), Ciprofloxacina (CI), Cloranfenicol (CL), Benzilpenicilina (PG), Amoxicilina + ácido clavulânico (XL), Doxiciclina (DC), Eritromicina (EM) e Azitromicina (AZ). Resultado: Todas as cepas clínicas testadas foram suscetíveis aos antibióticos AC, XL, PG, DC, MX, TC e VA. Todos os isolados das espécies de S. aureus foram suscetíveis aos 12 antibióticos testados. As cepas de E. faecalis, E. faecium e A. viscosus mostraram padrão de suscetibilidade intermediário contra EM. Algumas cepas de E. faecalis e E. faecium foram resistentes a AZ e RI. Conclusão: As cepas clínicas isoladas dos canais radiculares de dentes com insucesso endodôntico mostraram perfis diferentes de suscetibilidade antimicrobiana e nenhum isolado de E. faecalis e E. faecium apresentou-se suscetível a AZ e EM.Descritores: Endodontia; bactéria; antibióticos. AbstractIntroduction: Bacteria associated with failure endodontic treatment are capable of acquiring and expressing resistance against antimicrobial agents usually used to treat infections, which makes necessary, in some cases, laboratory tests in order to detect the resistance or antimicrobial susceptibility of these microorganisms. Objective: to evaluate the antimicrobial susceptibility of Enterococcus faecalis, Enterococcus faecium, Actinomyces viscosus and Staphylococcus aureus strains isolated from root canals of teeth with failure endodontic treatment. Material and method: Enterococcus faecalis (n=3), Enterococcus faecium (n=3), Actinomyces viscosus (n=3) and Staphylococcus aureus (n=3) clinical strains collected in vivo from root-filled canals with failure endodontic treatment thad been their antimicrobial susceptibility tested by the E-test method in duplicate using the antibiotics: Amoxicillin (AC), Rifampicin (RI), Moxifloxacin (MX), Vancomycin (VA), Tetracycline (TC), Ciprofloxacin (CI), Chloranphenicol (CL), Benzilpenicillin (PG), Amoxicillin + clavulanic acid (XL), Doxycycline (DC), Erythromycin (EM) e Azithromycin (AZ). Result: all the clinical strains tested were susceptib...

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New Bacterial Compositions in Root-filled Teeth with Periradicular Lesions

Cited by 100 publications

References 39 publications

Antimicrobial photodynamic therapy using visible light plus water-filtered infrared-A (wIRA)

Antimicrobial photodynamic therapy using visible light plus water-filtered infrared-A (wIRA)

Comparison of New and Conventional Root Canal Irrigants and their Efficacy on Treatment Outcomes in Endodontics

Avaliação da suscetibilidade antimicrobiana de bactérias anaeróbias facultativas isoladas de canais radiculares de dentes com insucesso endodôntico frente aos antibióticos de uso sistêmico

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