New Basal Media for Protocorm-Like Body and Callus Induction of Hybrid Cymbidium

Abstract

A B S T R A C THigh frequency protocorm-like body (PLB) production from hybrid Cymbidium Twilight Moon 'Day Light' has been developed through a new medium, Teixeira Cymbidium (TC) medium. Two new TC media containing variable amounts of macroand micronutrients and other additives, inspired by Winarto and Teixeira (WT) medium for Anthurium and Murashige and Skoog (MS) basal medium were used to induce PLBs and callus. Control medium was research-and industry-standard Vacin and Went (VW) medium. The first TC medium, TC PLB , could induce significantly more PLBs than on VW while high levels of macronutrients in the second TC medium, TC CALLUS , and MS were required to induce callus. All PLB induction media contained 0.1 mg/l α-naphthaleneacetic acid (NAA) and 0.1 mg/l kinetin (KIN), 2 g/l tryptone and 20 g/l sucrose, and solidified with 8 g/l Bacto agar while callusinduction media were identical, except that KIN was substituted by thidiazuron (TDZ). Basal medium had a significant effect on PLB and callus formation. This protocol could be used to induce PLBs and callus from other Cymbidium species or cultivars.

PENGARUH EKSTRAK TOMAT TERHADAP PERTUMBUHAN EMBRIO ANGGREK Phaius tankervilleae KHAS GUNUNG GALUNGGUNG KABUPATEN TASIKMALAYA

“…Menurut George dan Sherington (1984), media dasar yang pada umumnya digunakan dan dikembangkan khusus untuk medium anggrek misalnya media Vacin dan Went. Beberapa unsur dibutuhkan untuk mendukung pertumbuhan yaitu pemberian nutrisi pada media tanam termasuk pada fase pertumbuhan embrio anggrek dengan menambahkan bahan organik (Muharyati et al, 2015;Setiawati et al, 2016) atau zat pengatur tumbuh (Lestari, 2011;Semiarti et al, 2015;Teixeira da Silva, 2013). Keberadaan unsur zat pengatur tumbuh dalam medium pertumbuhan sangat menentukan pertumbuhan eksplan.…”

Sonication (Ultrasound) Affects In Vitro Growth of Hybrid Cymbidium

“…(Teixeira da silva, 2012b), optimized for this cultivar, was used in this study. All media were adjusted to pH 5.3 with 1 N NaOH or HCl prior to autoclaving at 100 kPa for 17 min.…”

Production of Synseed for Hybrid Cymbidium Using Protocorm-Like Bodies

“…PLBs of hybrid Cymbidium Twilight Moon 'Day Light' (Bio-U, Japan) originally developed spontaneously from shoot-tip culture on Vacin and Went (VW; Vacin and Went, 1949) agar medium without PGRs, were induced and subcultured (PLB induction and proliferation medium or VW PLB ) every two months on Teixeira Cymbidium (TC) medium (Teixeira da Silva, 2012) supplemented with 0.1 mg/l α-naphthaleneacetic acid (NAA) and 0.1 mg/l kinetin (KIN), 2 g/l tryptone and 20 g/l sucrose, and solidified with 8 g/l Bacto agar (Difco Labs., USA), according to procedures and advice outlined by Teixeira da and Teixeira da Silva and Tanaka (2006). All media were adjusted to pH 5.3 with 1 N NaOH or HCL prior to autoclaving at 100 KPa for 17 min.…”

“…This study focuses on single layer hydrogel encapsulation of a hybrid Cymbidium PLBs under several conditions and variables and subsequent germination on Teixeira Cymbidium (TC) medium (Teixeira da Silva, 2012) to establish the efficiency of a mid-to long-term storage choice for this ornamental crop. PLBs (apolar explant) pose a risk to survival during germination unlike polar explants such as somatic embryos or unipolar explants such as shoot tips (Sharma et al, 2012), although the risk of somaclonal variation is considerably reduced (Standardi and Piccioni, 1998).…”