New Breeding Lines Resistant to Tomato Mosaic Virus and Tomato Spotted Wilt Virus within the ‘De la Pera’ Tomato Type: UMH 1353 and UMH 1354

UMH1400 and UMH1401: New Cherry Tomato Breeding Lines Resistant to Virus

“…No significant differences have been found for the average fruit weight, TA and SSC. In previous works, breeding lines without TYLCV resistance had higher TA and SSC (ranging between 5% and 15%) than recurrent parentals in some crop cycles, both 'Muchamiel' (García-Martínez et al, 2015) and 'De la pera' types (García-Martínez et al, 2016). Comparing the breeding lines, UMH1400 shows significant decreases with respect to UMH1401 in marketable yield (ranging between 48% and 65%), average fruit weight (ranging between 15% and 33%), fruit number per plant (ranging between 35% and 63%), TA (ranging between 19% and 26%) and SSC (ranging between 10% and 17%) (Table 2).…”

“…'Anastasia F 1 ' was used as the donor parent of the Tm-2a, Sw-5, and Ty-1 genes (P erez de Castro et al, 2007), conferring resistance to ToMV, TSWV, and TYLCV, respectively. Twelve generations of backcrossing were performed to the cultivar cherry pera using Cleaved Amplified Polymorfhic Sequences (CAPS) markers for assisted selection for the virus resistance genes (García-Martínez et al, 2016). Several trials were carried out under different infection conditions (mechanical inoculation for ToMV and natural infection for TSWV) to check for the presence of resistance alleles in the first backcross (BC) generations and to assess the effectiveness of the molecular markers.…”

“…Several trials were carried out under different infection conditions (mechanical inoculation for ToMV and natural infection for TSWV) to check for the presence of resistance alleles in the first backcross (BC) generations and to assess the effectiveness of the molecular markers. Progenies for each backcross generation were screened with molecular markers for Tm-2a, Sw-5, and Ty-1 genes, described in García-Martínez et al (2016). In each BC progenie, only plants containing the three resistance genes (usually between 5 and 10 plants) were transplanted and then crossed with the recurrent parent cherry pera.…”

UMH1209 and UMH1155: New ‘Moruno Pera’ Tomato Breeding Lines Resistant to Virus

“…Anastasia F 1 was used as the donor parent of the Tm-2a, Sw-5, and Ty-1 genes (P erez de Castro et al, 2007), conferring resistance to ToMV, TSWV, and TYLCV, respectively. Six generations of backcrosses were performed to the 'Pera294' cultivar using Cleaved Amplified Polymorfhic Sequences (CAPS) markers described in (García-Martínez et al, 2016) for assisted selection for the virus resistance genes. In each BC progeny, only plants containing the three resistance genes (usually between five and 10 plants) were transplanted and then crossed with the recurrent parent 'Pera294'.…”

Twenty Years of Tomato Breeding at EPSO-UMH: Transfer Resistance from Wild Types to Local Landraces—From the First Molecular Markers to Genotyping by Sequencing (GBS)

“…The morphological characteristics and quality of these lines are similar to those found in the traditional varieties they are derived from, and there is no drop in production like that which occurs in UMH 1200, and in UMH 1203. These lines are therefore of great interest for cultivation purposes in TYLCV-free conditions, which can be achieved in a greenhouse with sufficient control measures, particularly in the spring season, when TYLCV occurs less frequently [44][45][46].…”