New capillary gel electrophoresis method for fast and accurate identification and quantification of multiple viral proteins in influenza vaccines

Tricht, Ewoud van; Geurink, Lars; Pajic, Bojana; Nijenhuis, Johan; Backus, H. H. J.; Germano, Marta; Somsen, Govert W.; Griend, Cari Sänger van de

doi:10.1016/j.talanta.2015.07.047

Cited by 25 publications

(39 citation statements)

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“…Intact proteins in biopharmaceutical products are commonly analyzed by spectrometry [14], electrophoresis [15], liquid chromatography [16,17], or mass spectrometry [14,[18][19][20]. More specifically, viral proteins are typically analyzed by SDS-PAGE [10,21,22], capillary gel electrophoresis (CGE) [23][24][25] or RP-HPLC [12,13,26]. SDS-PAGE has been extensively used for adenovirus protein analysis [27,28].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, protein modifications (for example, detected by MS) could be introduced by the harsh SDS-PAGE and MS pretreatment conditions. Capillary gel electrophoresis was applied for the analysis of recombinant GB virus-C proteins [29], the analysis of the four major influenza virus proteins [25], and the characterization of proteins from Western, Eastern, and Venezuelan equine encephalitis (WEVEE) virus like particles (VLPs) [23]. The downside of the capillary gel electrophoresis methods is the limited molecular weight range.…”

Section: Introductionmentioning

confidence: 99%

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Fast, selective and quantitative protein profiling of adenovirus-vector based vaccines by ultra-performance liquid chromatography

Tricht

Raadt

Verwilligen

et al. 2018

Journal of Chromatography A

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a b s t r a c tA method for the quantitative determination of the protein composition of adenovirus-vector based vaccines was developed. The final method used RP-UPLC with UV absorbance detection, a C4 column (300 Å, 1.7 m, 2.1 × 150 mm), and a water-acetonitrile (ACN) gradient containing trifluoroacetic acid (TFA) as ion-pairing agent. The chromatographic resolution between the various adenovirus proteins was optimized by studying the effect of the TFA concentration and the column temperature, applying a full factorial design of experiments. A reproducible baseline separation of all relevant adenovirus proteins could be achieved within 17 min run time. Samples containing adenovirus particles could be directly injected into the UPLC system without sample pretreatment. The viruses reproducibly dissociate into proteins in the UPLC system upon contact with the mobile phase containing ACN. The new RP-UPLC method was successfully validated for protein profiling and relative quantification of proteins in vaccine products based on adenovirus vector types 26 and 35. The intermediate precision of the relative peak areas of all proteins was between 1% and 14% RSD, except for the peak assigned to protein V (26% RSD). The method proved to be stability indicating with respect to thermal and oxidation stress of the adenovirusvector based vaccine and was successfully implemented for the characterization of adenovirus-based products.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fast, selective and quantitative protein profiling of adenovirus-vector based vaccines by ultra-performance liquid chromatography

Tricht

Raadt

Verwilligen

et al. 2018

Journal of Chromatography A

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“…An extensive research program on the topic has identified cooperation as the intuitive response in anonymous one-shot social dilemma experiments, with further reflection leading to more selfish choices (Rand et al, 2012 , 2014 , 2015 ; Cone and Rand, 2014 ; Rand and Kraft-Todd, 2014 ; Evans et al, 2015 ). These findings have led to the Social Heuristics Hypothesis (SHH; Rand et al, 2014 ), according to which people internalize social behaviors that generate personal benefits in daily life.…”

Section: Introductionmentioning

confidence: 99%

The cognitive basis of social behavior: cognitive reflection overrides antisocial but not always prosocial motives

Corgnet

Espín

Hérnan-Gonzalez

2015

Front. Behav. Neurosci.

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Even though human social behavior has received considerable scientific attention in the last decades, its cognitive underpinnings are still poorly understood. Applying a dual-process framework to the study of social preferences, we show in two studies that individuals with a more reflective/deliberative cognitive style, as measured by scores on the Cognitive Reflection Test (CRT), are more likely to make choices consistent with “mild” altruism in simple non-strategic decisions. Such choices increase social welfare by increasing the other person's payoff at very low or no cost for the individual. The choices of less reflective individuals (i.e., those who rely more heavily on intuition), on the other hand, are more likely to be associated with either egalitarian or spiteful motives. We also identify a negative link between reflection and choices characterized by “strong” altruism, but this result holds only in Study 2. Moreover, we provide evidence that the relationship between social preferences and CRT scores is not driven by general intelligence. We discuss how our results can reconcile some previous conflicting findings on the cognitive basis of social behavior.

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“…63 The analyses were performed in uncoated capillaries using a commercial gel kit (AB Sciex). In the optimized CGE described in this paper, the influenza proteins were injected hydrodynamically at 100 mbar for 100 s which provided similar results (based on peak area, S/N, and migration time) compared to electrokinetic injection (EKI) at -18 kV for 100 s. However, hydrodynamic injection (HDI) is less affected by sample matrix and provides better precision.…”

Section: Separation Modes and Conditionsmentioning

confidence: 99%

Recent advances in protein analysis by capillary and microchip electrophoresis

Dawod

Arvin

Kennedy

2017

Analyst

120

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This review article describes the significant recent advances in analysis of proteins by capillary and microchip electrophoresis during the period mid 2014 to early 2017. The review highlights the progressions, new methodologies, innovative instrumental modifications, and challenges for efficient protein analysis in human specimens, animal tissues, and plant samples. Protein analysis fields covered in this review include analysis of native, reduced, and denatured proteins in addition to Western blotting, protein therapeutics and proteomics.

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New capillary gel electrophoresis method for fast and accurate identification and quantification of multiple viral proteins in influenza vaccines

Cited by 25 publications

References 21 publications

Fast, selective and quantitative protein profiling of adenovirus-vector based vaccines by ultra-performance liquid chromatography

Fast, selective and quantitative protein profiling of adenovirus-vector based vaccines by ultra-performance liquid chromatography

The cognitive basis of social behavior: cognitive reflection overrides antisocial but not always prosocial motives

Recent advances in protein analysis by capillary and microchip electrophoresis

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