New Catechol Derivatives of Safrole and Their Antiproliferative Activity towards Breast Cancer Cells

Abstract

Catechols were synthesized from safrole. Nine derivatives were prepared and assessed for antiproliferative effects using different human cell lines. The in vitro growth inhibition assay was based on the sulphorhodamine dye to quantify cell viability. The derivatives 4-allylbenzene-1,2-diol (3), 4 4-[3-(acetyloxy)propyl]-1,2-phenylene diacetate (6) and 4-[3-(acetyloxy)propyl]-5-nitro-1,2-phenylene diacetate (10) showed higher cytotoxicity than the parent compound 2 in tests performed on two breast cancer cell lines (MCF-7 and MDA-MB-231). The IC50 values of 40.2 ± 6.9 μM, 5.9 ± 0.8 μM and 33.8 ± 4.9 μM, respectively, were obtained without toxicity towards dermal human fibroblast (DHF cells).

Antioxidant and Anti-Proliferative Activity of Essential Oil and Main Components from Leaves of Aloysia polystachya Harvested in Central Chile

“…The panel of cancer cells were seeded at a density of 2 × 10 4 cells/well into 96-well plates and assayed as described previously [ 59 ]. Test compounds were solubilized just prior to the experiment in 0.1% DMSO.…”

Synthesis and Evaluation of Novel Oxyalkylated Derivatives of 2′,4′-Dihydroxychalcone as Anti-Oomycete Agents against Bronopol Resistant Strains of Saprolegnia sp.

“…Unless otherwise stated, all chemical reagents and positive controls purchased (Aldrich, Darmstadt, Germany) were of the highest commercially available purity and were used without previous purification. Structures of synthesized products were confirmed by spectroscopic methods have been given elsewhere [ 29 ]. The course of synthesis was controlled by means of thin layer chromatography and products were separated by column chromatography following a standard method [ 29 ].…”

“…Structures of synthesized products were confirmed by spectroscopic methods have been given elsewhere [ 29 ]. The course of synthesis was controlled by means of thin layer chromatography and products were separated by column chromatography following a standard method [ 29 ].…”

Synthesis and Antiproliferative Activity of New Cyclodiprenyl Phenols against Select Cancer Cell Lines

“…They included human prostate cancer cells (PC-3), breast carcinoma cells (MCF-7), human colorectal adenocarcinoma cells (HT-29), human dermal fibroblast cells (HDF) and human colonic epithelial cells (CoN). Cells were grown by the procedure previously described in reference [ 48 ].…”