New closed tube loop mediated isothermal amplification assay for prevention of product cross-contamination

Karthik, Kumaragurubaran; Rathore, Rajesh; Thomas, Prasad; Arun, T.R.; Viswas, K.N.; Dhama, Kuldeep; Agarwal, Rajesh

doi:10.1016/j.mex.2014.08.009

Cited by 99 publications

(76 citation statements)

References 14 publications

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“…LAMP has been designed for various pathogens and has also been developed for detection of Arcobacter spp. Karthik et al 2014b;Wang et al 2014). Results showed that the developed LAMP assay was more sensitive than the other molecular assays like multiplex PCR and conventional PCR that were compared in the study (Wang et al 2014).…”

Section: Detection Of Arcobacter Spp By Molecular Methodsmentioning

confidence: 89%

Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control – a comprehensive review

Ramees

Dhama

Karthik

et al. 2017

Veterinary Quarterly

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138

130

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Arcobacter has emerged as an important food-borne zoonotic pathogen, causing sometimes serious infections in humans and animals. Newer species of Arcobacter are being incessantly emerging (presently 25 species have been identified) with novel information on the evolutionary mechanisms and genetic diversity among different Arcobacter species. These have been reported from chickens, domestic animals (cattle, pigs, sheep, horses, dogs), reptiles (lizards, snakes and chelonians), meat (poultry, pork, goat, lamb, beef, rabbit), vegetables and from humans in different countries. Arcobacters are implicated as causative agents of diarrhea, mastitis and abortion in animals, while causing bacteremia, endocarditis, peritonitis, gastroenteritis and diarrhea in humans. Three species including A. butzleri, A. cryaerophilus and A. skirrowii are predominantly associated with clinical conditions. Arcobacters are primarily transmitted through contaminated food and water sources. Identification of Arcobacter by biochemical tests is difficult and isolation remains the gold standard method. Current diagnostic advances have provided various molecular methods for efficient detection and differentiation of the Arcobacters at genus and species level. To overcome the emerging antibiotic resistance problem there is an essential need to explore the potential of novel and alternative therapies. Strengthening of the diagnostic aspects is also suggested as in most cases Arcobacters goes unnoticed and hence the exact epidemiological status remains uncertain. This review updates the current knowledge and many aspects of this important food-borne pathogen, namely etiology, evolution and emergence, genetic diversity, epidemiology, the disease in animals and humans, public health concerns, and advances in its diagnosis, prevention and control.

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Section: Detection Of Arcobacter Spp By Molecular Methodsmentioning

confidence: 89%

Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control – a comprehensive review

Ramees

Dhama

Karthik

et al. 2017

Veterinary Quarterly

Self Cite

138

130

View full text Add to dashboard Cite

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“…LAMP assay has the intrinsic property of product carry over contamination hence a novel closed tube LAMP assay was also developed for detection of Brucella spp. (Karthik et al, 2014b). LAMP assay for specific diagnosis of B. abortus was also developed which was 100 fold more sensitive than the commonly employed PCR (Karthik et al, 2014c).…”

Section: Serological Tests Like Rose Bengal Plate Test (Rbpt) Standamentioning

confidence: 99%

Equine brucellosis: Review on epidemiology, pathogenesis, clinical signs, prevention and control

Karthik¹,

Prabakar²,

Bharathi³

et al. 2016

JEBAS

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KEYWORDS Brucellosis Equine B. abortus Poll evil Fistulous withers LAMP ABSTRACTBrucellosis is one of the major zoonotic diseases that affect several domestic animals, wild animals and also marine mammals. Though there is no specific Brucella sp. that can affect horses, B. abortus and B. suis can affect horses naturally and B. canis experimental infection has also been reported in equines. Brucellosis in equines is characterized by two conditions namely Poll evil and fistulous withers. Organism has its predilection for joints, ligaments and tendons in case of equines and causes inflammatory conditions leading to formation of fistula. Equine brucellosis has been documented from several parts of the world and prevalence has been reported time to time mostly based on serological diagnosis. Diagnosis of brucellosis mainly depends on serological methods though isolation of the organism is the gold standard. Due to hazardous nature of the pathogen, tests like Rose Bengal plate agglutination test, Standard tube agglutination test and other serological assays are commonly employed. Isothermal amplification assay like LAMP are gaining momentum these years due to swiftness in diagnosis of the pathogen. LAMP with high specificity and sensitivity for detection of Brucella spp. and also B. abortus has been developed in the recent years. Prevention and control of brucellosis is of utmost important to halt the spread of the organism to other animals and human. Trauma is a major reason for predisposition of poll evil and fistulous withers hence proper fitting of saddle will help to prevent the disease. Housing and feeding the horses separately can prevent spread of disease from cattle. The present review discusses equine brucellosis, its epidemiology, pathogenesis, clinical signs along with appropriate prevention and control strategies to be adapted.

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“…False positives observed in our study, could also stem from cross-contamination between positive and negative samples in the final stage of the test run. It was shown by Karthik et al (2014) that it is possible to contaminate working area when tubes are opened after isothermal amplification for addition of fluorescent dye. Replacing SYBR Green with calcein or hydroxyl methyl blue added before reaction (Goto et al 2009) could eliminate this problem.…”

Section: Resultsmentioning

confidence: 99%

Application of loop-mediated isothermal amplification (LAMP) assays for the detection of bovine herpesvirus 1

Socha¹,

Rola²,

Urban‐Chmiel³

et al. 2017

Polish Journal of Veterinary Sciences

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Bovine herpesvirus-1 (BoHV-1), a causative agent of Infectious Bovine Rhinotracheitis (IBR), is responsible for high economic losses in cattle farming industry. The use of testing methods that allow early detection of BoHV-1-infected animals is a key element of each program of IBR eradication. The aim of the study was to design and evaluate two variants of LAMP isothermal tests with SYBR Green fluorescence probes, specific to the genes encoding gD and gE glycoproteins of BoHV-1. LAMP gE BoHV-1 assay was able to distinguish between gE-and gE+ strains of the virus. Both LAMP gD and gE assays were specific to BoHV-1 and did not react with other related to BoHV-1 alphaherpesviruses. Sensitivity of LAMP gD was 2x10 4 copies of the viral genome whereas for LAMP gE it was 2x10 5 . Diagnostic sensitivity calculated for LAMP gD was 64.7% whereas for LAMP gE it was 80%. Diagnostic specificity for LAMP gD and LAMP gE was 78.9% and 89.3%, respectively. LAMP assay can be a rapid and simple method of diagnosis of acute BoHV-1 infections and discrimination of gE -strains.However, relatively low diagnostic sensitivity of the method can limit its use in routine diagnostics.

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New closed tube loop mediated isothermal amplification assay for prevention of product cross-contamination

Abstract: Graphical abstract

Cited by 99 publications

References 14 publications

Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control – a comprehensive review

Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control – a comprehensive review

Equine brucellosis: Review on epidemiology, pathogenesis, clinical signs, prevention and control

Application of loop-mediated isothermal amplification (LAMP) assays for the detection of bovine herpesvirus 1

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