New contributions to the relationship between sequential changes of ATP-related metabolites and post-mortem interval in rats

Zhu, Weihao; Zhai, Xiandun; Zheng, Zhe; Sun, Kai; Yang, Meng; Mo, Yaonan

doi:10.1016/j.legalmed.2020.101809

Cited by 3 publications

(6 citation statements)

References 30 publications

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“…Zhu et al presented that ADP level did not significant change within the first 8 h but within 16-24 h the ADP increased. 6 At the 2-12 h, the AMP level significant decreased after that the AMP level significant increased (p<0.05). Zhu et.al showed that over all of the AMP level trended to increase in the first 144 h after death.…”

Section: Resultsmentioning

confidence: 84%

“…4 The ATP level with in the first 8 h increased following not significant change within 8-16 h in skeletal rat muscle model. 6 However, these results from in vitro model presented that the ATP decreased after 12 h and need to confirm in the crops study.…”

Section: Resultsmentioning

confidence: 88%

“…Zhu et.al showed that over all of the AMP level trended to increase in the first 144 h after death. 6 Energy is an important biochemical molecule for the adequate turnover of the biomolecular structures and the functional metabolic viability in unicellular organisms. ATP, ADP and AMP level and energy charge (EC) reflect to the energetic status of the cell.12 The EC value in physiological system presents in range 0.7-0.9 and reflects to a balance with the major ATP-consuming reaction.…”

Section: Resultsmentioning

confidence: 99%

“…9 After death, a biochemical changes will occur under the influence of various intrinsic and environmental factors. 6 These processes are nonreversible, progressive and time-sequence.…”

Section: Resultsmentioning

confidence: 99%

“…The ATP synthesis was stopped and rapidly degraded to adenosine diphosphate (ADP), adenosine monophosphate (AMP) which can be summarized as: ATP→ADP→AMP. [5][6] An extensive metabolomics and biochemical change in all body tissue due to lack of circulating oxygen, altered enzymatic reactions, cellular degradation and cessation of anabolic production of metabolites after death. The biochemical changes provide information about the effect of death on cellular function especially ATP blood level.…”

Section: Introductionmentioning

confidence: 99%

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Changing of ATP and Its Metabolites in Blood Samples for Post Mortem Interval: In Vitro Study

Warangkool Chanpan,

Churdsak Jaikang,

Chaturong Kanchai

2022

Indian Journal of Forensic Medicine & Toxicology

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Background: Postmortem interval (PMI) is an important in forensic practice. Many studies in blood and tissue of animals have revealed correlation between adenosine triphosphate (ATP) level and PMI. In this study aimed to explore the correlation between human blood ATP and its metabolites level and PMI in vitro model. Methods:Ten milliliter of venous blood samples were collected form four healthy men and contained in EDTA tube. One milliliter of the blood sample was divided at 0, 1, 2, 4, 8, 12 and 24 h, respectively. The blood samples were extracted and measured ATP, adenosine diphosphate (ADP) and adenosine monophosphate (AMP) level by High performance liquid chromatography-diode array. Energy charge value was calculated to predict charge of ATP metabolites. Results:The blood ATP, ADP and AMP levels increased in the first hour. The blood ATP, ADP and AMP levels did not obviously change before 12 hour. The blood ATP level trended to decrease but the blood ADP and AMP levels trended to increase after 24 hour. Energy Charge was not change in first 12 hour but trended to decrease after 24 hour. Conclusion:The blood ATP level and its metabolites changed after 12 hour and apply for the PMI investigation.

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Section: Resultsmentioning

confidence: 84%

Section: Resultsmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 99%

“…9 After death, a biochemical changes will occur under the influence of various intrinsic and environmental factors. 6 These processes are nonreversible, progressive and time-sequence.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Changing of ATP and Its Metabolites in Blood Samples for Post Mortem Interval: In Vitro Study

Warangkool Chanpan,

Churdsak Jaikang,

Chaturong Kanchai

2022

Indian Journal of Forensic Medicine & Toxicology

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A novel method for determining postmortem interval based on the metabolomics of multiple organs combined with ensemble learning techniques

Wei

et al. 2022

Int J Legal Med

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Informativeness of postmortem dynamics of skeletal muscles of dog and cat corpses for forensic veterinary diagnosis of death due to acute hypoxia

Yatsenko,

Kazantsev

2024

Ukrainian journal of veterinary sciences

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The relevance of the study is conditioned by the need in the practice of forensic veterinary expertise to establish the statute of limitations for the occurrence of death of dogs and cats during the investigation of crimes against animal health and life. In this regard, the purpose of the study is to solve diagnostic forensic veterinary problems regarding the informative value of postmortem disorganisation of skeletal neck muscles of dog and cat corpses within 72 hours after the death of animals. The main methods of investigating the diagnostic information content of postmortem dynamics of skeletal muscles of dog and cat corpses for forensic veterinary determination of the conditions and prescription of death due to acute hypoxia are those that can be comprehensively considered the identified problem, in particular, Papenheim-Kryukov staining of smears, slides – hematoxylin and eosin, fluorescein isothiocyanate, and Shabadash staining. The forensic veterinary diagnostic significance of two expert criteria is justified: “the number of patterns of disorganisation of the neck muscles of dog and cat corpses” and “glycogen content in the neck muscles of dog and cat corpses”. It is proved that biotransformation of skeletal muscles occurs in the following sequence: during the first 24 hours after the death of the animal, postmortem stiffness of the muscles of corpses occurs, accompanied by dehydration, compaction, contraction of myofibrils; muscle relaxation is observed from 24 to 48 hours after death, myofibrils are stretched and swollen; from 48 to 72 hours, autolysis of muscles is recorded under the influence of saprotrophs. It was found that the destruction of skeletal muscle tissue is more intense in the case of death due to suffocation compared to death from poisoning, and the destruction of muscle tissue is more dynamic in the case of death due to freezing compared to death caused by drowning in cold water. For the first time, the results obtained will contribute to solving diagnostic forensic veterinary tasks, in particular, establishing the statute of limitations and conditions for the occurrence of death of dogs and cats during the investigation by law enforcement agencies of crimes related to animal cruelty

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New contributions to the relationship between sequential changes of ATP-related metabolites and post-mortem interval in rats

Cited by 3 publications

References 30 publications

Changing of ATP and Its Metabolites in Blood Samples for Post Mortem Interval: In Vitro Study

Changing of ATP and Its Metabolites in Blood Samples for Post Mortem Interval: In Vitro Study

A novel method for determining postmortem interval based on the metabolomics of multiple organs combined with ensemble learning techniques

Informativeness of postmortem dynamics of skeletal muscles of dog and cat corpses for forensic veterinary diagnosis of death due to acute hypoxia

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