New Derivative Spectrophotometric Methods for the Determination of Fluoxetine - An Antidepressant Drug

Annapurna, Mukthinuthalapati Mathrusri; Pradhan, Debi Prasad

doi:10.7598/cst2012.225

Cited by 6 publications

(2 citation statements)

References 2 publications

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“…These assays/techniques were reviewed and published in some review articles [5,6]. Among these assays, spectrophotometric assays are the most widely used, as indicated from the number of published articles describing spectrophotometric assays for SSRIs [7][8][9][10][11][12][13][14][15][16][17][18][19]. The great interest in spectrophotometric assays for SSRIs is due to their simplicity, convenience, low cost, ready automation with spectrophotometric analyzers, and availability of the technique in almost all quality control laboratories in pharmaceutical manufacturing companies [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of Green and High-Throughput Microwell Spectrophotometric Assay for the Determination of Selective Serotonin Reuptake Inhibitors in Their Pharmaceutical Dosage Forms

Darwish

Alzoman

2023

Molecules

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This study describes the development and validation of a new green and high-throughput microwell spectrophotometric assay (MW-SPA) for the determination of three selective serotonin reuptake inhibitors (SSRIs) in their pharmaceutical dosage forms. These SSRIs are fluoxetine, fluvoxamine, and paroxetine, the most prescribed drugs for the treatment of depression. The proposed assay was based on the formation of orange-colored N-substituted naphthoquinone derivatives upon the reaction of SSRIs with 1,2-naphthoquinone-4-sulphonate (NQS) in alkaline media. The assay was conducted in 96-microwell assay plates, and the absorbances of the reaction products were measured by a microplate reader at their maximum absorbance wavelengths. The optimum conditions of the reaction were refined and established. Under these conditions, calibration curves were generated, and linear regression equations were computed. The linear relations between the absorbances and drug concentrations were linear with good correlation coefficients (0.9992–0.9997) in the range of 2–80 µg/mL. The assay limits of detection were in the range of 1.5–4.2 µg/mL. The precision was satisfactory as the values of relative standard deviation did not exceed 1.70%. The accuracy of the assay was ≥98.2%. The proposed MW-SPA was successfully applied to the analysis of the SSRIs in their pharmaceutical dosage forms with acceptable accuracy and precision; the label claims were 99.2–100.5% (±0.96–1.35%). The results of the proposed MW-SPA were compared with those of the official/pre-validated assays by statistical analysis with respect to the accuracy (by t-test) and precision (by F-test). No significant differences were found between the calculated and theoretical values of the t- and F-tests at the 95% confidence level, proving similar accuracy and precision in the determination of SSRIs by both assays. The greenness of the proposed assay was confirmed by two metric tools. In addition, the assay is characterized with a high-throughput property which enables the simultaneous analysis of many samples in a short time. Therefore, the assay is a valuable tool for rapid routine application in pharmaceutical quality control units for the determination of the investigated SSRIs.

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Section: Introductionmentioning

confidence: 99%

Development and Validation of Green and High-Throughput Microwell Spectrophotometric Assay for the Determination of Selective Serotonin Reuptake Inhibitors in Their Pharmaceutical Dosage Forms

Darwish

Alzoman

2023

Molecules

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“…Only few methods were reported for the determination of LIN in tablets, including spetrophotometric (18), TLC (19) and HPLC (20) methods, either alone or in the presence of alkaline induced degradation products. Estimation of LIN in biological fluids using LC/MS (21) and HPLC (22) was also described.…”

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confidence: 99%

Spectrofluorimetric determination of gemifloxacin mesylate and linezolid in pharmaceutical formulations: Application of quinone-based fluorophores and enhanced native fluorescence

Moussa¹,

Mahrouse²,

Hassan³

et al. 2014

Acta Pharmaceutica

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Quinone-based fluorophores and enhanced native fluorescence techniques were applied for a fast quantitative analysis of gemifloxacin mesylate (GEM) and linezolid (LIN) in pharmaceutical formulations. For this purpose, three sensitive, accurate and precise spectrofluorimetric methods were developed. GEM, as an n-electron donor, reacts with 7,7,8,8-tetracyanoquinodimethane (method A) and 2,5-dichloro-3,6-dihydroxy-p-benzoquinone (method B) as п-electron acceptors, forming charge transfer complexes that exhibit high fluorescence intensity at 441 and 390 nm upon excitation at 260 and 339 nm, respectively. Method C depends on measurement of enhanced native fluorescence of LIN in phosphate buffer (pH 5) at 380 nm upon excitation at 260 nm. Experimental factors affecting fluorescence intensity were optimized. Linearity was obtained over concentration ranges 50-500, 10-60 and 20-400 ng mL-1 for methods A, B and C, respectively. The developed methods were validated and successfully applied for determination of the cited drugs in tablets.

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Development of dispersive liquid–liquid microextraction with solid-phase evaporation as a novel hyphenated method prior to ion mobility spectrometry and its application for trace analysis of fluoxetine

et al. 2023

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New Derivative Spectrophotometric Methods for the Determination of Fluoxetine - An Antidepressant Drug

Cited by 6 publications

References 2 publications

Development and Validation of Green and High-Throughput Microwell Spectrophotometric Assay for the Determination of Selective Serotonin Reuptake Inhibitors in Their Pharmaceutical Dosage Forms

Development and Validation of Green and High-Throughput Microwell Spectrophotometric Assay for the Determination of Selective Serotonin Reuptake Inhibitors in Their Pharmaceutical Dosage Forms

Spectrofluorimetric determination of gemifloxacin mesylate and linezolid in pharmaceutical formulations: Application of quinone-based fluorophores and enhanced native fluorescence

Development of dispersive liquid–liquid microextraction with solid-phase evaporation as a novel hyphenated method prior to ion mobility spectrometry and its application for trace analysis of fluoxetine

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