New Diaz Coupling Reaction, Cloud Point Extraction Spectrophotometric Determination of Sulphadimidine Soudium in Pure form and Pharmacetical Preparation with Salicylic Acid as the Coupling Reaction

Abstract

In this research we study two simple methods, rapid of use spectrophotometric determination of sulphadimadine sodium (SDMS). The fi rst method based on diazotization of drug by sodium nitrite at 5Cº followed by coupling with Salicylic Acid in basic medium to form yellow color. The product was measured at 453 nm. Beer's law is obeyed in the concentration range of (1-16) μg•ml -1 . Sandell's sensitivity was 0.07575 μg•cm -1 , the detection limit was 0.3992 μg•ml -1 , and the limit of Quantitation was 1.07879μg•ml -1 . The second method was used cloud point extraction (CPE) with used Triton X-114 as surfactant. Beer's law obeyed in the range of concentration was (1-12) μg•ml -1. Sandell's sensitivity was 0.04 μg•cm -1 , the detection limit was 0.0259 μg•ml -1 , and the limit of quantitation was 0.029563 μg•ml -1. All variables were study including of the reagent concentration, reaction time. The composition of product (1:1). The methods were effectively useful to the determination of SDMS in pharmaceutical dose form, and the attained results were in good agreement with the offi cial and other methods in literature .No interference was observed from the commonly encountered additives and recipients.

A New Green Approach of CFIA Technique for Direct Assay with a High Throughput of Sulfamethoxazole Drugs Using Condensation Reaction with NQS Agent

“…For the spectrophotometric determination of the colored product the stoichiometry was to calculate the ratio between the drug and reagent using obs and the mole ratio methods 20 . The results obtained as in Fig.…”