New Diffuse Reflectance Infrared Fourier Transform Spectroscopy for the Estimation of Tramadol Hydrochloride Capsules

Abstract

Objective: The objective of the study was to develop and validate a new diffuse reflectance infrared fourier transform (DRIFT) spectroscopic method for the quantification of Tramadol HCl in the pharmaceutical dosage form. Methods: The Fourier Transform Infrared (FTIR) analysis was carried out on Cary 630 FTIR spectrophotometer (Agilent Technologies, USA) equipped with a diffuse reflectance sampling interface (650-4000 cm-1, 32 scans, 8 cm-1 resolution). The solid-state samples were prepared by dilution in dry potassium bromide and were analysed by an FTIR spectrophotometer with the DRIFT sampling technique, which follows the Kubelka-Munk model depicting the theory of diffuse reflectance at scattering surfaces, relating band intensities to concentration for transmission measurements similar to Beer’s law. Results: A linear relationship was found in the selected wavenumber range of 3295-3298 cm-1denoting the carboxyl peak in the concentration range of 0.6-3.0% w/w with a good correlation coefficient of 0.997. The percent recovery of Tramadol HCl in the marketed dosage form was found to be 100.4%. Conclusion: The proposed method was found to be accurate, precise, reproducible, and eco-friendly. DRIFT spectroscopy may have the potential as an alternative method for qualitative and quantitative analysis of Tramadol HCl in its capsules.

Development and Validation of Fast and Simple Fourier Transform Infrared Spectrophotometric Method for Analysis of Thiamphenicol in Capsule Dosage Form

“…Infrared is a spectrophotometric method and vibrational spectroscopy Fadlelmoula et al (2022) with many advantages such as being selective, easy, fast, simple, environmentally friendly, and nondestructive (Estupiñán Méndez and Allscher, 2022) . Infrared spectroscopy is a popular technique for analyzing various sample matrixes, namely pharmaceutical products (Siregar et al, 2018;Burela andMandalemula, 2023), food (Sahachairungrueng et al, 2022;Mendes and Duarte, 2021), biological liquids (Kamnev et al, 2021) , or environmental samples (Tkachenko and Niedzielski, 2022) . Infrared spectrophotometry analysis can determine the functional groups in the compound (Enders et al, 2021) and predict the chemical reaction (Zeaiter et al, 2022) .…”