New Disc Format for Biosensing by Using Optical Pick-Up Head System

Huang, Der‐Ray; Ju, Jau Jiu; Lee, Yuan Chin; Chen, Jiin Sen; Lo, Feng Hsiang; Chang, Sheng Li

doi:10.1109/tmag.2014.2300340

Cited by 1 publication

(3 citation statements)

References 4 publications

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“… 75 Besides the PMT, the OPU can also equip an avalanche photodiode for Cy5 detection of stained cell fluorescence on a spinning disc. 76 …”

Section: Opu-based Biosensingmentioning

confidence: 99%

“…The imaging depth can be adjusted in a range of ±20 μm through collimator lens B4 (Figure ) inside the OPU . Besides the PMT, the OPU can also equip an avalanche photodiode for Cy5 detection of stained cell fluorescence on a spinning disc …”

Section: Opu-based Biosensingmentioning

confidence: 99%

“…75 Besides the PMT, the OPU can also equip an avalanche photodiode for Cy5 detection of stained cell fluorescence on a spinning disc. 76 Nanoparticle labeling can enhance the biomolecule signals up to 100 times. 77 Using this labeling technique, OPUs can scan the DNA microarray with an external photodiode, which is much less expensive than a PMT.…”

Section: Acs Sensorsmentioning

confidence: 99%

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Hacking CD/DVD/Blu-ray for Biosensing

Hwu

Boisen

2018

ACS Sens.

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The optical pickup unit (OPU) within a CD/DVD/Blu-ray drive integrates 780, 650, and 405 nm wavelength lasers, diffraction-limited optics, a high-bandwidth optoelectronic transducer up to 400 MHz, and a nanoresolution x-, z-axis, and tilt actuator in a compact size. In addition, the OPU is a remarkable piece of engineering and could enable different scientific applications such as sub-angstrom displacement sensing, micro- and nanoimaging, and nanolithography. Although off-the-shelf OPUs can be easily obtained, manufacturers protect their datasheets under nondisclosure agreements to impede their availability to the public. Thus, OPUs are black boxes that few people can use for research, and only experienced researchers can access all their functions. This review details the OPU mechanism and components. In addition, we explain how to utilize three commercially available triple-wavelength OPUs from scratch and optimize sensing quality. Then, we discuss scientific research using OPUs, from standard optical drive-based turnkey-biomarker array reading and OPU direct bioapplications (cytometry, optical tweezing, bioimaging) to modified OPU-based biosensing (DNA chip fluorescence scanning, biomolecular diagnostics). We conclude by presenting future trends on optical storage devices and potential applications. Hacking low-cost and high-performance OPUs may spread micro- and nanoscale biosensing research from research laboratories to citizen scientists around the globe.

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“… 75 Besides the PMT, the OPU can also equip an avalanche photodiode for Cy5 detection of stained cell fluorescence on a spinning disc. 76 …”

Section: Opu-based Biosensingmentioning

confidence: 99%

Section: Opu-based Biosensingmentioning

confidence: 99%