New epicardial mapping electrode with warming/cooling function for experimental electrophysiology studies

Tormos, Álvaro; Guill, Antonio; Millet, José; Roses, Eduardo J.; Trapero, Isabel; Such-Miquel, Luis; Chorro, Francisco J.

doi:10.1016/j.medengphy.2010.12.019

Cited by 8 publications

(5 citation statements)

References 23 publications

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“…After several tests, we corroborated that the cause of this effect was the Tyrode that oozed at 37 ºC, typical of the Langendorf System where the excess effluent accumulates in the right ventricle and is expelled through the pulmonary vein, oozing all over the epicardium. We were aware of this effect but its impact in previous experiments [3] and [7] had been minimal. In contrast to previous experiments that utilized a larger surface with screen-printed electrodes, our system uses tiny peltier cells arranged in an array with the HD Grid multi-electrode.…”

Section: Resultsmentioning

confidence: 95%

“…One of the possible variables to achieve this purpose is temperature. The local temperature drop applied to the epicardium in experiments with perfused rabbit hearts showed a decreasing effect on the local dominant frequency of the fibrillatory recordings [3] without affecting the normothermic zones. It has been provided acceptable linear correlation between temperature and dominant frequency values, as well as decreased conduction velocity with hypothermia in sinus rhythm [7].…”

Section: Introductionmentioning

confidence: 89%

“…Finally, the product of the mass with the specific heat allows us to obtain the thermal capacity of the hot side, the cold side, and the semiconductor element [4] [6]. The complete circuit is shown in Fig 1 B, considering the effect of the thermal capacity of each component forming the Peltier cell and its equations ( 1), (2), and (3).…”

Section: B Peltier Cell Modelingmentioning

confidence: 99%

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Mini Peltier Cell Array System for the Generation of Controlled Local Epicardial Heterogeneities

Segarra,

Cebrián,

Ruipérez-Campillo

et al. 2023

2023 45th Annual International Conference of the IEEE Engineering in Medicine &Amp; Biology Society (EMBC)

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The present study aims to design and fabricate a system capable of generating heterogeneities on the epicardial surface of an isolated rabbit heart perfused in a Langendorff system. The system consists of thermoelectric modules that can be independently controlled by the developed hardware, thereby allowing for the generation of temperature gradients on the epicardial surface, resulting in conduction slowing akin to heterogeneities of pathological origin. A comprehensive analysis of the system's viability was performed through modeling and thermal simulation, and its practicality was validated through preliminary tests conducted at the experimental cardiac electrophysiology laboratory of the University of Valencia. The design process involved the use of Fusion 360 for 3D designs, MATLAB/Simulink for algorithms and block diagrams, LTSpice and Altium Designer for schematic captures and PCB design, and the integration of specialized equipment for animal experimentation. The objective of the study was to efficiently capture epicardial recordings under varying conditions.Clinical relevance-The proposed system aims to induce local epicardial heterogeneities to generate labeled correct signals that can serve as a golden standard for improving algorithms that identify and characterize fibrotic substrates. This improvement will enhance the efficacy of ablation processes and potentially reduce the ablated surface area.

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Section: Resultsmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 89%

Section: B Peltier Cell Modelingmentioning

confidence: 99%

See 1 more Smart Citation

Mini Peltier Cell Array System for the Generation of Controlled Local Epicardial Heterogeneities

Segarra,

Cebrián,

Ruipérez-Campillo

et al. 2023

2023 45th Annual International Conference of the IEEE Engineering in Medicine &Amp; Biology Society (EMBC)

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“…Those with lower quality, noise, or artifacts were discarded. In each experiment, a self-manufactured multielectrode consisting of 128 stainless steel electrodes (interelectrode distance 1 mm; diameter 0.125 mm) [ 29 ] was positioned on the epicardial surface of the anterior wall of the left ventricle. A bipolar epicardial stimulating electrode was used (diameter, 0.125 mm; interelectrode distance, 1 mm), always positioned at the same location, proximally to the external lateral side of the recording electrode (Fig.…”

Section: Methodsmentioning

confidence: 99%

Evaluation and assessment of clique arrangements for the estimation of omnipolar electrograms in high density electrode arrays: an experimental animal model study

et al. 2023

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High-density catheters combined with Orientation Independent Sensing (OIS) methods have emerged as a groundbreaking technology for cardiac substrate characterisation. In this study, we aim to assess the arrangements and constraints to reliably estimate the so-called omnipolar electrogram (oEGM). Performance was evaluated using an experimental animal model. Thirty-eight recordings from nine retrospective experiments on isolated perfused rabbit hearts with an epicardial HD multielectrode were used. We estimated oEGMs according to the classic triangular clique (4 possible orientations) and a novel cross-orientation clique arrangement. Furthermore, we tested the effects of interelectrode spacing from 1 to 4 mm. Performance was evaluated by means of several parameters that measured amplitude rejection ratios, electric field loop area, activation pulse width and morphology distortion. Most reliable oEGM estimations were obtained with cross-configurations and interelectrode spacings $$\le 2$$ ≤ 2 mm. Estimations from triangular cliques resulted in wider electric field loops and unreliable detection of the direction of the propagation wavefront. Moreover, increasing interelectrode distance resulted in increased pulse width and morphology distortion. The results prove that current oEGM estimation techniques are insufficiently accurate. This study opens a new standpoint for the design of new-generation HD catheters and mapping software.

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“…A standard way to characterize these ex-vivo tissues is through the use of perfusion chambers for isolated tissues [4], or the use of the Langendorff perfused model for small animal hearts, often utilized in combination with an electrode array [5], an optical mapping system with voltage sensitive dye [6], or one lead ECG recordings [7]. These methods, however, are usually expensive and require specialized instrument systems.…”

Section: Introductionmentioning

confidence: 99%

An inexpensive alternative bath system for electrophysiological characterization of isolated cardiac tissue

Villa

White

Liao

et al. 2011

2011 Annual International Conference of the IEEE Engineering in Medicine and Biology Society

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A tissue bath system, to be used as an alternative to complex perfusion chambers, was constructed for use in cardiac electrophysiological studies. This system consists of an acrylic chamber to hold circulating physiological medium such as DMEM, suspended in a water bath warmed by a hot plate. Temperature and pH were controlled to mimic physiological conditions. Rat and porcine cardiac tissues, were used to test viability of the conditions presented in the bath system. Using a cardiac mapping system, the tissues were stimulated and responses recorded. From the recordings we were able to calculate conduction velocities and spatial dispersion of activation indices. The results are comparable to previous in-vivo work, which suggests that the tissue bath system design can maintain tissue viability. This tissue bath system is a relatively simple alternative for ex-vivo testing of cardiac tissues.

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New epicardial mapping electrode with warming/cooling function for experimental electrophysiology studies

Cited by 8 publications

References 23 publications

Mini Peltier Cell Array System for the Generation of Controlled Local Epicardial Heterogeneities

Mini Peltier Cell Array System for the Generation of Controlled Local Epicardial Heterogeneities

Evaluation and assessment of clique arrangements for the estimation of omnipolar electrograms in high density electrode arrays: an experimental animal model study

An inexpensive alternative bath system for electrophysiological characterization of isolated cardiac tissue

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