1982
DOI: 10.1038/295160a0
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New Epstein–Barr virus variants from cellular subclones of P3J-HR-1 Burkitt lymphoma

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Cited by 148 publications
(120 citation statements)
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“…HH514-16 is a subclone of the P3J-HR1K BL cell line. This subclone was selected for absence of spontaneous EBV lytic replication and a high level of chemically induced EBV lytic-cycle gene expression (28). All cell lines were grown in RPMI 1640 medium containing 8% fetal bovine serum and antibiotics.…”
Section: Methodsmentioning
confidence: 99%
“…HH514-16 is a subclone of the P3J-HR1K BL cell line. This subclone was selected for absence of spontaneous EBV lytic replication and a high level of chemically induced EBV lytic-cycle gene expression (28). All cell lines were grown in RPMI 1640 medium containing 8% fetal bovine serum and antibiotics.…”
Section: Methodsmentioning
confidence: 99%
“…Virus stocks were obtained from the P3J-HR1 Burkitt's lymphoma cellular subclone 13 which when treated with 12-O-tetradecanoylphorbol-13-acetate (TPA) releases large amounts of virus into culture supernatant fluids (Heston et al, 1982). A tritiated EBV stock was prepared from clone 13 maintained 2 days in medium containing 760 lag/ml hypoxanthine and 18 p.g/ml aminopterin and induced with 30 ng/ml TPA in the presence of 2 ~Ci/ml [3H]thymidine (sp.…”
Section: Methodsmentioning
confidence: 99%
“…A detailed analysis of EBV gene expression in Mg68 has previously been reported (49). The cell line 253.30 is an LCL transformed by a mini-EBV plasmid, p1244.8a (32), packaged in transformation-defective HH514 helper virus (a subclone of P3HR1) (26). This plasmid was derived from 71 kb of noncontiguous portions of the B95-8 genome (163,477 to 19,359, 43,935 to 56,081, and 79,658 to 113,282) cloned into a recombinant F-factor-based Escherichia coli plasmid by a chromosome-building technique previously described in detail (32,47).…”
mentioning
confidence: 99%