New Escherichia coli WP2 tester strains highly sensitive to reversion by oxidative mutagens

Blanco, Manuel; Urios, Amparo; Martínez, Antonio

doi:10.1016/s1383-5718(98)00013-8

Cited by 48 publications

(53 citation statements)

References 18 publications

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“…It has been claimed that the negative results which occurred when D5 was tested for mutagenicity using E. coli strain WP2 uvrA indicate that D5 does not cause mutations due to oxidative DNA damage. However, the parent E. coli strain WP2 uvrA has been demonstrated to be relatively insensitive to oxidative DNA damage (Blanco et al, 1998;Martinez et al, 2000). This suggests the need for further testing to determine if D5 is capable of causing oxidative DNA damage.…”

Section: D5 Cancer Risk Evaluationmentioning

confidence: 99%

Distribution, Elimination, and Rearrangement of Cyclic Volatile Methylsiloxanes in Oil-Contaminated Soil of the Shengli Oilfield, China

Shi

et al. 2015

Environ. Sci. Technol.

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SUBJECT: REVIEW OF TOXICITY INFORMATION ON D5 ______________________________________________________________________________We are forwarding our review of available information on the toxicity and persistence of decamethylcyclopentasiloxane (D5), a proposed alternative for perchloroethylene in dry cleaning. The review was conducted to provide ARB with information on which to base a determination of whether D5 could be considered a non-toxic alternative to perchloroethylene for dry cleaning under AB 998 (Lowenthal, Chapter 821, Statutes of 2003), pursuant to contract number 05-414. In response to your request to evaluate available information on the toxicity of D5 under the statutory mandate, OEHHA staff have reviewed information submitted by the Silicones Environmental Health and Safety Council (SEHSC), including studies evaluating acute and subchronic toxicity, neuroendocrine activity, estrogenicity, genotoxicity, chronic toxicity and carcinogenicity and related mode of action studies, and pharmacokinetics. In addition, we evaluated an SEHSC white paper summarizing the toxicity of D5, and an exposure assessment conducted by Environ Corporation for SEHSC. We also searched the open literature for additional information, including government documents from other countries, and identified additional information on human exposure, environmental persistence and accumulation in biota. As requested by ARB, we focused on evaluating the applicability of the SEHSC-proposed mechanism of action of tumor formation in rodents to human health risk assessment. Our review of the available information is attached.In the process of reviewing the information, we met three times with the SEHSC representatives, including their toxicologists. We sought outside expertise from the University of California, Davis, regarding the merits of the proposed mode of action of D5 induced tumors in rodents. We also spoke with U.S.EPA scientists, who reviewed materials on D5 toxicity under

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Section: D5 Cancer Risk Evaluationmentioning

confidence: 99%

Distribution, Elimination, and Rearrangement of Cyclic Volatile Methylsiloxanes in Oil-Contaminated Soil of the Shengli Oilfield, China

Shi

et al. 2015

Environ. Sci. Technol.

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“…Test is performed on both OxyR proficient IC185 and OxyR deficient IC202 strains [61]. The OxyR protein is a redox-sensitive transcriptional activator of genes encoding antioxidative enzymes: catalase-hydroperoxidase I, alkyl hydroperoxide reductase and glutathione reductase [77]. Mutants in oxyR are deficient in inducible expression of antioxidant enzymes and thus very sensitive for detection of oxidative mutagens and antimutagenic effect of antioxidants.…”

Section: Other Reversion Testsmentioning

confidence: 99%

Molecular Mechanisms of Action of Antimutagens from Sage (Salvia officinalis) and Basil (Ocimum basilicum)

Nikolić¹,

Mitić-Ćulafić²,

Vuković-Gačić³

et al. 2012

Mutagenesis

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“…Both media were solidified with 1.5% Difco bacto agar. E medium (Vogel J Biol Chem 1956) [10] solidified with 1.5% Difco bacto agar was supplemented with histidine for the Ames test (Maron Mutat Res 1983) [11] or tryptophan for the WP2 mutagenicity test (Blanco Mutat Res 1998) [12] assays. A selective minimal medium containing 0.4% lactose was used for the lac + -revertant mutagenesis assay (Cupples PNAS 1989) [13] (Cupples Genetics 1990 [14].…”

Section: Bacterial Mediamentioning

confidence: 99%

“…This assay was carried out as described by Blanco et al (Mutat Res 1998) [12], using the tryptophan auxotroph strains WP2s, IC203, IC204, IC206 e IC208 of E. coli ( Table 1). Each experimental determination was performed in duplicate, and results represent the average of at least three independent experiments.…”

Section: Tryptophan Mutagenesis Assaymentioning

confidence: 99%

Genotoxic and Mutagenic Effects of Melissa officinalis (Erva Cidreira) Extracts

Alves¹,

Vidal²,

Küster³

et al. 2009

TOTOXIJ

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Erva cidreira (Melissa officinalis) is a plant with sedative properties and in Brazil it has been used for insomnia and anxiety. It is also employed to stimulate liver functions and normalize menstruation and for intestinal constipation. The aim of this work was to evaluate the genotoxic and mutagenic effects of erva cidreira. Results indicate the presence of genotoxic activity in the lyophilized extract, with lysogenic induction increasing about 80-fold over the spontaneous background after treatment with lyophilized total aqueous extract. Beta-galactosidase levels raised 9-fold in indicator strains after treatment under the same conditions. A deeper look into the mechanism underlying the genotoxic action implicated reactive oxygen species generated by compounds retained in the ethyl-acetate, polar partition of the total aqueous extract. The spectrum of mutations revealed a great trend for base substitutions, mainly in guanines and adenines. The presence of phenolic compounds, especially caffeic acid derivatives, may be correlated to its genotoxic activity. We thus conclude that compounds present in current concentrations of erva cidreira extracts are genotoxic and mutagenic, and might have carcinogenic potential.

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New Escherichia coli WP2 tester strains highly sensitive to reversion by oxidative mutagens

Cited by 48 publications

References 18 publications

Distribution, Elimination, and Rearrangement of Cyclic Volatile Methylsiloxanes in Oil-Contaminated Soil of the Shengli Oilfield, China

Distribution, Elimination, and Rearrangement of Cyclic Volatile Methylsiloxanes in Oil-Contaminated Soil of the Shengli Oilfield, China

Molecular Mechanisms of Action of Antimutagens from Sage (Salvia officinalis) and Basil (Ocimum basilicum)

Genotoxic and Mutagenic Effects of Melissa officinalis (Erva Cidreira) Extracts

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