New frontiers in thermal analysis

Materazzi, Stefano; Gullifa, Giuseppina; Fabiano, Maria Aurora; Frati, Paola; Santurro, Alessandro; Scopetti, Matteo; Fineschi, Vittorio; Risoluti, Roberta

doi:10.1007/s10973-017-6239-y

Cited by 29 publications

(16 citation statements)

References 43 publications

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“…Thermogravimetric techniques have been largely applied to complex matrix as analytical tool that does not require sample pretreatment nor clean-up (Toth et al, 2010;Fonseca et al, 2012;Materazzi et al, 2015Materazzi et al, , 2017aPapadopoulos et al, 2016;Risoluti et al, 2016bRisoluti et al, , 2017Bach and Chen, 2017;Catauro et al, 2018) and permits to rapidly obtain both qualitative and quantitative outcomes (Skreiberg et al, 2011;Materazzi et al, 2014a,b;Napoli et al, 2014;Shan-Yang et al, 2015;Marcilla et al, 2017). Recently, a novel approach based on the association of thermogravimetric analysis (TGA) and chemometrics has been proposed in different fields (Pappa et al, 2003;Khanmohammadi et al, 2012;Caramés-Alfaya et al, 2013;Chauhan et al, 2020), including the investigation of biological samples for clinical analysis (Risoluti et al, 2016a(Risoluti et al, , 2018aMaterazzi et al, 2017a). Themogravimetry, in fact, with respect to clinical specimens, presents the advantage of processing samples without requiring any sample manipulation and the resulting curves reflect the characteristic composition of the sample in few microliters of sample.…”

Section: Introductionmentioning

confidence: 99%

An Innovative Multilevel Test for Hemoglobinopathies: TGA/Chemometrics Simultaneously Identifies and Classifies Sickle Cell Disease From Thalassemia

Risoluti

Caprari

Gullifa

et al. 2020

Front. Mol. Biosci.

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Introduction: Hemoglobinopathies are the most common genetic disorder wordlwide and because of migrations are become an emerging global health problem. Screening programmes for Sickle cell disease and Thalassemia have been implemented in some countries, but are not a common practice, due to a lack in the accuracy of the methods and to the costs of the analyses. Objectives: The objective of this study was the application of the thermogravimetry coupled to chemometrics as new screening method to perform an early diagnosis of thalassemia and sickle cell disease. Methods: Whole blood samples (30 µL) from sickle cell anemia and thalassemia patients were analyzed using the thermobalance TG7 and the resulting curves were compared with those of healthy individuals. A chemometric approach based on Principal Components Analysis (PCA) was exploited to enhance correlation between thermogravimetric profiles and a model of prediction by Partial Least Square Discriminant Analysis (PLS-DA) was developed and validated. Results: The characteristic profile of the blood sample thermal decomposition and the first derivative of the TG curve showed that patients were clearly distinguished from healthy individuals as a result of different amounts of water and corpuscular fraction of blood. The chemometric approach based on PCA allowed a quick identification of differences between healthy subjects and patients and also between thalassemic and sickle cell anemia subjects. Chemometric tools (PLS-DA) were used to validate a model of prediction to process the thermogravimetric curves and to obtain in 1 h an accurate diagnosis. The TGA/Chemometric test permitted to perform first level test for hemoglobinopathies with the same accuracy of confirmatory analyses obtained by the molecular investigation. Conclusions: A screening test based on the coupling of thermogravimetry and chemometrics was optimized for the differential diagnosis of hemoglobinopathies. The novel test is able to simultaneously perform a simple and fast diagnosis of sickle cell Risoluti et al. Innovative Multilevel Test for Hemoglobinopathies anemia or thalassemia, in a single analysis of few microliters of non-pretreated whole blood at low cost, and with high accuracy. Moreover this method results particularly suitable in pediatric patients as it requires small sample volumes and is able to characterize also transfused patients.

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Section: Introductionmentioning

confidence: 99%

An Innovative Multilevel Test for Hemoglobinopathies: TGA/Chemometrics Simultaneously Identifies and Classifies Sickle Cell Disease From Thalassemia

Risoluti

Caprari

Gullifa

et al. 2020

Front. Mol. Biosci.

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“…Thermal analysis is recognized as instrumental method of food analysis able to give unique information regarding the nature of the sample or the modifications induced by industrial processing. Books and reviews report the applications of thermoanalytical techniques to the food science [22][23][24][25][26][27][28]. Thermal analysis has been extensively applied to characterize the thermal, structural, and rheological properties of the chocolates [3,[8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Thermogravimetric characterization of dark and milk chocolates at different processing stages

Ostrowska–Ligęza

Górska

Wirkowska‐Wojdyła

et al. 2018

J Therm Anal Calorim

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The effect of different processing stages of dark and milk chocolate samples on their thermal properties was investigated. The ingredients of chocolates were investigated too. Thermal behavior was evaluated by means of thermogravimetry (TG) and first-derivative thermogravimetry (DTG). The measurements were made at a temperature range of 50-700°C with a heating rate of 10°C min -1 . TG and DTG are thermal techniques, which have been taken advantage of research cocoa liquor, sugar, and cocoa butter in chocolates during the different processing stages. The obtained results evidenced possibility of indicating differences between milk fat and cocoa butter contribution and content of cocoa liquor and sugar which differ for dark and milk chocolates.

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“…Multivariate statistical analysis based on Principal Component Analysis (PCA; Risoluti et al, 2016a; Materazzi et al, 2017c) was used as exploratory method, while Partial Least Square Linear Discriminant Analysis (PLS-DA; Savitzky and Golay, 1964; Barker and Rayens, 2003; Materazzi et al, 2017a,b) was applied as the classification model of prediction. Each blood sample was analyzed three times and data was reported as means ± standard deviations.…”

Section: Methodsmentioning

confidence: 99%

TGA/Chemometric Test Is Able to Detect the Presence of a Rare Hemoglobin Variant Hb Bibba

Risoluti

Caprari

Gullifa

et al. 2019

Front. Mol. Biosci.

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In this study the TGA/Chemometric test was applied for diagnosis of a case of congenital hemolytic anemia for which the common first level diagnostic tests were not able to find the erythrocyte congenital defect. A 6 years old girl presented chronic hemolytic anemia characterized by hyperbilirubinemia, increased spleen, negative Coombs tests, normal hemoglobin values, decreased mean corpuscular volume (MCV), increased red cell distribution width (RDW), reticulocytes and lactate dehydrogenase (LDH), and altered erythrocyte morphology (ovalocytes, spherocytes, and rare schizocytes). The diagnostic protocols for differential diagnosis of hereditary hemolytic anemia were carried out by the investigation of the congenital hemolytic anemias due to defects of membrane proteins and the most common erythrocyte enzymes, but no defect was found. The TGA/Chemometric test was applied and the PLS-DA model of prediction was used to process results. The thermogravimetric profile of the patient was very distinct from those of healthy subjects and comparable with those of thalassemia patients. The classification model applied to the patient identified a chronic hemolytic anemia due to a hemoglobin defect and the molecular characterization confirmed the TGA/Chemometrics results, demonstrating the presence of a very rare hemoglobin variant Hb Bibba (α2136(H19)Leu → Proβ2). In conclusion the TGA/Chemometric test proved to be a promising tool for the screening of the hemoglobin defects, in a short time and at low cost, of this case of congenital hemolytic anemia of difficult diagnosis. This method results particularly suitable in pediatric patients as it requires small sample volumes and is able to characterize patients subjected to transfusion.

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New frontiers in thermal analysis

Cited by 29 publications

References 43 publications

An Innovative Multilevel Test for Hemoglobinopathies: TGA/Chemometrics Simultaneously Identifies and Classifies Sickle Cell Disease From Thalassemia

An Innovative Multilevel Test for Hemoglobinopathies: TGA/Chemometrics Simultaneously Identifies and Classifies Sickle Cell Disease From Thalassemia

Thermogravimetric characterization of dark and milk chocolates at different processing stages

TGA/Chemometric Test Is Able to Detect the Presence of a Rare Hemoglobin Variant Hb Bibba

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