New Global Insights on the Regulation of the Biphasic Life Cycle and Virulence Via ClpP-Dependent Proteolysis in Legionella pneumophila

Ge, Zhenhuang; Yuan, Peibo; Chen, Lingming; Chen, Junyi; Dong, Shen; She, Zhigang; Lü, Yongjun

doi:10.1016/j.mcpro.2022.100233

Cited by 7 publications

(7 citation statements)

References 194 publications

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“…They referred to such microbial behavior as a biphasic life cycle. Evidence of a biphasic life cycle has been found in B. bacteriovorus, where significant metabolic changes were observed when comparing predating bacteria with intraperiplasmic bacteria of the same strain ( Herencias et al, 2020 ) and in virulent versus non-virulent L. pneumophila ( Ge et al, 2022 ).…”

Section: Discussionmentioning

confidence: 98%

Adaptive lifestyle of bacteria determines phage-bacteria interaction

et al. 2022

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Bacteriophages and their interactions with microbes are not well understood. As a first step toward achieving a better understanding, we isolated and sequenced the Curvibacter phage PCA1 for the purpose of eliminating Curvibacter sp. AEP1.3, the main colonizer of Hydra vulgaris AEP. Our experiments showed that PCA1 phage caused a strong, virulent infection only in sessile Curvibacter sp. AEP1.3 but was unable to infect planktonic and host-associated bacterial cells of the same strain. In an effort to investigate this phenomenon, we compared sessile, planktonic, and host-associated bacteria via RNA sequencing and found that all three states differed significantly in their expression patterns. This finding led us to propose that the adaptive lifestyle of Curvibacter sp. AEP1.3 results in varying degrees of susceptibility to bacteriophage infection. This concept could be relevant for phage research and phage therapy in particular. Finally, we were able to induce phage infection in planktonic cells and pinpoint the infection process to a membrane protein. We further identified potential phage-binding protein candidates based on expression pattern analysis.

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Section: Discussionmentioning

confidence: 98%

Adaptive lifestyle of bacteria determines phage-bacteria interaction

et al. 2022

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“…Table 1 compiles the main regulatory systems described that play an important role in L. pneumophila infection in amoeba and in the biofilm life cycle. Proteomics and transcriptomics studies revealed key metabolic pathways, common to in vivo infection models and in vitro broth cultures, that dictate the phenotypic shift from the replicative to the transmissive phase [ 48 ]. This phenotype transition is coordinated by regulatory systems that control gene expression, such as regulatory proteins (CsrA, RpoS, FliA and FleQ), the LetA/LetS (LetA/S) two-component system ( Legionella transmission activator and sensor, respectively) the stringent response metabolites (RelA, SpoT and ‘ppGpp’) and noncoding/small RNA (nc/sRNA) [ [49] , [50] , [51] , [52] , [53] ].…”

Section: Pneumophila Biphasic Life Cyclementioning

confidence: 99%

Ecology of Legionella pneumophila biofilms: The link between transcriptional activity and the biphasic cycle

Barbosa,

Azevedo,

Goeres

et al. 2024

Biofilm

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“…There are two subclasses of the T4SS, namely the T4SS -A (Lvh) and the T4SS-B (Dot/Icm:(Defect in organelle trafficking/Intracellular multiplication). The Dot/Icm genes encode Dot/Icm secretory system proteins [ 67 ], and are vital for organelle trafficking and intracellular multiplication [ 80 ], while the Lvh secretion system contains genes encoding mobility factors and enzymes [ 81 ]. Furthermore, the Lvh can functionally replace defective Dot/Icm.…”

Section: The Mechanism Of Ldmentioning

confidence: 99%

Legionella pneumophila: The Journey from the Environment to the Blood

Iliadi

Staykova

Iliadis

et al. 2022

JCM

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An outbreak of a potentially fatal form of pneumonia in 1976 and in the annual convention of the American Legion was the first time that Legionella spp. was identified. Thereafter, the term Legionnaires’ disease (LD) was established. The infection in humans is transmitted by the inhalation of aerosols that contain the microorganisms that belong to the Legionellaceae family and the genus Legionella. The genus Legionella contains genetically heterogeneous species and serogroups. The Legionella pneumophila serogroup 1 (Lp1) is the most often detected strain in outbreaks of LD. The pathogenesis of LD infection initiates with the attachment of the bacterial cells to the host cells, and subsequent intracellular replication. Following invasion, Legionella spp. activates its virulence mechanisms: generation of specific compartments of Legionella-containing vacuole (LCV), and expression of genes that encode a type IV secretion system (T4SS) for the translocation of proteins. The ability of L. pneumophila to transmigrate across the lung’s epithelium barrier leads to bacteremia, spread, and invasion of many organs with subsequent manifestations, complications, and septic shock. The clinical manifestations of LD depend on the bacterial load in the aerosol, the virulence factors, and the immune status of the patient. The infection has two distinct forms: the non- pneumatic form or Pontiac fever, which is a milder febrile flu-like illness, and LD, a more severe form, which includes pneumonia. In addition, the extrapulmonary involvement of LD can include heart, brain, abdomen, and joints.

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New Global Insights on the Regulation of the Biphasic Life Cycle and Virulence Via ClpP-Dependent Proteolysis in Legionella pneumophila

Cited by 7 publications

References 194 publications

Adaptive lifestyle of bacteria determines phage-bacteria interaction

Adaptive lifestyle of bacteria determines phage-bacteria interaction

Ecology of Legionella pneumophila biofilms: The link between transcriptional activity and the biphasic cycle

Legionella pneumophila: The Journey from the Environment to the Blood

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