New Granulocyte Antigens Demonstrated by Microgranulocytotoxicity Assay

Thompson, John S.; Overlin, V. L.; Herbick, J. M.; Severson, Charles D.; Claas, Frans H.J.; Amaro, Jony; Burns, C. Patrick; Rg, Strauss; Koepke, J.A.

doi:10.1172/jci109807

Cited by 47 publications

(15 citation statements)

References 15 publications

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“…very large numbers of leukocytes obtained following G-CSF plus corticosteroid donor stimulation). However, as reported previously [6,7], up to 80% of recipients of antiquated granulocyte transfusions (i.e. donors either not stimulated at all or only with adrenal corticosteroids) at our institution exhibited leukocyte antibodies, and it is likely that leukocyte alloimmunization will continue to occur in the future.…”

Section: Questionsupporting

confidence: 73%

Granulocyte Transfusions

2000

Vox Sanguinis

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Section: Questionsupporting

confidence: 73%

Granulocyte Transfusions

2000

Vox Sanguinis

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“…It was voluntarily withdrawn from the US market in 2000 due to its side-effect profile, which includes idiosyncratic agranulocytosis and the development of vasculitic lesions with prolonged exposure (7)(8)(9). Agranulocytosis was observed at rates of 2.5 to 13% in patients treated with moderate to high doses for protracted periods (10). Although the exact mechanism remains unclear, anti-neutrophil antibodies have been described in some patients (11,12).…”

Section: Introductionmentioning

confidence: 99%

Contaminated Cocaine and Antineutrophil Cytoplasmic Antibody-Associated Disease

McGrath

Isakova

Rennke

et al. 2011

Clinical Journal of the American Society of Nephrology

166

151

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SummaryBackground and objectives Approximately 70% of illicit cocaine consumed in the United States is contaminated with levamisole. Most commonly used as a veterinary antihelminthic agent, levamisole is a known immunomodulating agent. Prolonged use in humans has been associated with cutaneous vasculitis and agranulocytosis. We describe the development of a systemic autoimmune disease associated with antineutrophil cytoplasmic antibodies (ANCA) in cocaine users. This complication appears to be linked to combined cocaine and levamisole exposure. Design, setting, participants, & measurements Cases were identified between March 2009and November 2010 at Massachusetts General Hospital's ANCA laboratory. Cocaine exposure was identified from patient history in all cases. Medical records were reviewed for clinical presentation and for laboratory and diagnostic evaluation.Results Thirty cases of ANCA positivity associated with cocaine ingestion were identified. All had antimyeloperoxidase antibodies and 50% also had antiproteinase 3 antibodies. Complete clinical and laboratory data were available for 18 patients. Arthralgia (83%) and skin lesions (61%) were the most frequent complaints at presentation. Seventy-two percent of patients reported constitutional symptoms, including fever, night sweats, weight loss, or malaise. Four patients had biopsy-proven vasculitis. Two cases of acute kidney injury and three cases of pulmonary hemorrhage occurred. From the entire cohort of 30, two cases were identified during the first 3 months of our study period and nine cases presented during the last 3 months. ConclusionsWe describe an association between the ingestion of levamisole-contaminated cocaine and ANCA-associated systemic autoimmune disease. Our data suggest that this is a potentially life-threatening complication of cocaine use.

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“…For this purpose we used, in addition to DNA transfection analyses (tumorigenicity assay), a dot-blot screening procedure based on a combination of in vitro amplification of RAS-specific sequences and hybridization to mutation-specific oligonucleotide probes (9)(10)(11) In one CMML patient we separated various cell fractions obtained from peripheral blood specimens-namely, lymphocytes, granulocytes, as well as monocytes/macrophages according to standard techniques (17)(18)(19). Slides were prepared from each cell fraction for evaluation of purity by May/Grunwald/Giemsa and Sudan black B stainings and revealed >90% purity for each sample.…”

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confidence: 99%

RAS gene mutations in acute and chronic myelocytic leukemias, chronic myeloproliferative disorders, and myelodysplastic syndromes.

Janssen

Steenvoorden

Lyons

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

213

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We report on investigations aimed at detecting mutated RAS genes in a variety of preleukemic disorders (1,5,6). Moreover, in all cases investigated thus far, the mutations turned out to be specific for the tumor cells and were not found in normal cells of the respective patient. Activation of RAS genes has been detected in a variety of different neoplasias with variable frequencies. By far, the highest incidence (25-50%) has been reported in acute myelocytic leukemia (AML) (6-8).To gain further information on the biological significance of RAS mutations in leukemogenesis, we investigated the occurrence of RAS gene mutations in AML compared to a broad spectrum of other preleukemic and leukemic disorders involving the myeloid lineage. For this purpose we used, in addition to DNA transfection analyses (tumorigenicity assay), a dot-blot screening procedure based on a combination of in vitro amplification of RAS-specific sequences and hybridization to mutation-specific oligonucleotide probes (9)(10)(11)

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New Granulocyte Antigens Demonstrated by Microgranulocytotoxicity Assay

Cited by 47 publications

References 15 publications

Granulocyte Transfusions

Granulocyte Transfusions

Contaminated Cocaine and Antineutrophil Cytoplasmic Antibody-Associated Disease

RAS gene mutations in acute and chronic myelocytic leukemias, chronic myeloproliferative disorders, and myelodysplastic syndromes.

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