New host for raspberry bushy dwarf virus: arctic bramble (Rubus arcticus)

Kokko, Hanna; Lemmetty, Anne; Haimi, Perttu; Kärenlampi, Sirpa

doi:10.1007/bf01877253

Cited by 15 publications

(5 citation statements)

References 14 publications

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“…raspberry hybrids, wild R. idaeus var. strigosus, R. occidentalis, R. parviflorus, R. leucodermis, R. multibracteatus, R. glaucus, R. arcticus) (Chamberlain et al 2003;Kokko et al 1996;Martin et al 2013;Quito-Avila et al 2013;Wall and Shamoun 1990). In 2003 the first natural RBDV infection of non-Rubus host was reported for grapevine from Slovenia (Mavrič et al 2003) and later also from Serbia and Hungary (Jevremović and Paunović 2011;Mavrič Pleško et al 2012;Czotter et al 2018).…”

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confidence: 99%

Raspberry bushy dwarf virus in Slovenia - geographic distribution, genetic diversity and population structure

Pleško

Lamovšek

Lešnik³

et al. 2020

Eur J Plant Pathol

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Raspberry bushy dwarf virus (RBDV) is a long-known virus naturally infecting Rubus and grapevine. It is also one of the economically most important viruses of raspberries, but there are only a limited number of sequences covering a substantial part of the genome available in the databases. The aim of this study was: i) to study the geographic distribution of RBDV in Slovenia, and ii) to sequence RNA2 of several red raspberry and grapevine RBDV isolates and study their phylogeny and population structure. Geographic distribution studies were performed over a period of 13 years in three wine-growing regions of Slovenia (Primorska, Podravje and Posavje). The highest incidence of RBDV was found in Podravje (58.8%) and the lowest in Primorska (5.1%). Big differences were observed between Vipavska dolina (10.2%) and three other wine-growing districts of Primorska region (0.4–1.2%). Almost complete RNA2 sequences were obtained for four red raspberry isolates and seven grapevine isolates. Additionally, only coat protein sequences were obtained for three red raspberry isolates. Phylogenetic and population diversity analyses were performed on all available RBDV sequences. Phylogenetic analysis has shown clear differences in sequences from Rubus and grapevine that form two highly supported clades. In RNA2 analysis additional two sub-clades were found in grapevine clade. Two major subclades were identified also in the Rubus clade with further differentiation within these subclades. Purifying or stabilizing selection was found to be acting on both, CP and MP genes while few codons were found to be under positive selection.

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mentioning

confidence: 99%

Raspberry bushy dwarf virus in Slovenia - geographic distribution, genetic diversity and population structure

Pleško

Lamovšek

Lešnik³

et al. 2020

Eur J Plant Pathol

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“…Of 40 analyzed plant samples, 17 were found to contain two or three viruses. RBDV and BRNV have been previously identified in both cultivated and wild raspberries in Finland [7, 21, 39, 40]. In addition, RBDV has been detected in arctic brambles ( Rubus arcticus L.) in Finland [39, 41].…”

Section: Discussionmentioning

confidence: 99%

Small-RNA analysis of pre-basic mother plants and conserved accessions of plant genetic resources for the presence of viruses

et al. 2019

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Pathogen-free stocks of vegetatively propagated plants are crucial in certified plant production. They require regular monitoring of the plant germplasm for pathogens, especially of the stocks maintained in the field. Here we tested pre-basic mother plants of Fragaria , Rubus and Ribes spp., and conserved accessions of the plant genetic resources of Rubus spp. maintained at research stations in Finland, for the presence of viruses using small interfering RNA (siRNA) -based diagnostics (VirusDetect). The advance of the method is that unrelated viruses can be detected simultaneously without resumptions of the viruses present. While no virus was detected in pre-basic mother plants of Fragaria and Ribes species, rubus yellow net virus (RYNV) was detected in pre-basic mother plants of Rubus . Raspberry bushy dwarf virus (RBDV), black raspberry necrosis virus (BRNV), raspberry vein chlorosis virus (RVCV) and RYNV were detected in the Rubus genetic resource collection. The L polymerase encoding sequence characterized from seven RVCV isolates showed considerable genetic variation. The data provide the first molecular biological evidence for the presence of RYNV in Finland. RYNV was not revealed in virus indexing by indicator plants, which suggests that it may be endogenously present in some raspberry cultivars. In addition, a putative new RYNV-like badnavirus was detected in Rubus spp. Blackcurrant reversion virus (BRV) and gooseberry vein banding associated virus (GVBaV) were detected in symptomatic Ribes plants grown in the field. Results were consistent with those obtained using PCR or reverse transcription PCR and suggest that the current virus indexing methods of pre-basic mother plants work as expected. Furthermore, many new viruses were identified in the collections of plant genetic resources not previously tested for viruses. In the future, siRNA-based diagnostics could be a useful supplement for the currently used virus detection methods in certified plant production and thus rationalize and simplify the current testing system.

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“…Idaeovirus rubi is one of two species in the genus Idaeovirus [1]. RBDV naturally infects members of the Rubus species, including black raspberry (R. occidentalis), blackberry (R. fruticosus), loganberry (R. loganobaccus), boysenberry (R. ursinus × idaeus), and arctic bramble (R. arcticus) [2][3][4][5], as well as grapevine [6] and cherry [7]. It is transmitted both horizontally and vertically through pollen [8,9].…”

Section: Introductionmentioning

confidence: 99%

Genetic Characterization of Raspberry Bushy Dwarf Virus Isolated from Red Raspberry in Kazakhstan

Kolchenko

Kapytina

Kerimbek

et al. 2023

Viruses

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Raspberry bushy dwarf virus (RBDV) is an economically significant pathogen of raspberry and grapevine, and it has also been found in cherry. Most of the currently available RBDV sequences are from European raspberry isolates. This study aimed to sequence genomic RNA2 of both cultivated and wild raspberry in Kazakhstan and compare them to investigate their genetic diversity and phylogenetic relationships, as well as to predict their protein structure. Phylogenetic and population diversity analyses were performed on all available RBDV RNA2, MP and CP sequences. Nine of the isolates investigated in this study formed a new, well-supported clade, while the wild isolates clustered with the European isolates. Predicted protein structure analysis revealed two regions that differed between α- and β-structures among the isolates. For the first time, the genetic composition of Kazakhstani raspberry viruses has been characterized.

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New host for raspberry bushy dwarf virus: arctic bramble (Rubus arcticus)

Cited by 15 publications

References 14 publications

Raspberry bushy dwarf virus in Slovenia - geographic distribution, genetic diversity and population structure

Raspberry bushy dwarf virus in Slovenia - geographic distribution, genetic diversity and population structure

Small-RNA analysis of pre-basic mother plants and conserved accessions of plant genetic resources for the presence of viruses

Genetic Characterization of Raspberry Bushy Dwarf Virus Isolated from Red Raspberry in Kazakhstan

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