New insight into the analysis of amniotic fluid microflora using 16S rRNA gene sequencing

You, Young Ah; Son, Ga Hyun; Kwon, Eun Jin; Park, Mi Hye; Lee, Keun Young; Kim, Young Ju

doi:10.1099/jmmcr.0.005021

Cited by 3 publications

(7 citation statements)

References 29 publications

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“…The set of oligonucleotide primers for the two targeted genes used showed no amplification, indicating that there was no presence of F. nucleatum species in the uterus. However, our finding is at variance with the previous studies by You et al (36) in Korea and that of Vander Haar et al (28) in which F. nucleatum was detected in amniotic fluid samples. None of the 16 amniotic fluids screened had F. nucleatum even though some of these samples were purulent and cloudy as opposed to the normal pale.…”

Section: Discussioncontrasting

confidence: 99%

Evaluation of Fusobacterium nucleatum in Amniotic Fluid of Women with Preterm Labour

Nwaokorie,

Okunade,

Edet

et al. 2023

Babcock Univ. Med. J.

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Objective: The effect of Fusobacterium nucleatum, an oral commensal, on pregnancy outcomes is of global concern. This study screened for F. nucleatum and the fadA gene in amniotic fluid obtained from women presenting with preterm labour. Methods: This is a cross-sectional pilot study. Amniotic fluid was collected from women with preterm labour at Lagos University Teaching Hospital and analysed using conventional polymerase chain reaction (PCR). I6SrRNA species-specific primers were used to assess the presence of F. nucleatum species and fadA genes for virulence potentials. Questionnaire-based data obtained was analysed using SPSS version 20.0. and amplicon analysis was measured on base pairs. Results: Fourty participants had preterm deliveries between April and August 2018. Of these 34 (85%) were enrolled, out of which 25 amniotic fluid samples (AF) were collected. The mean age of the participants was 30.5 years. Among them, 10 (29.4%) were primigravida, and 24 (70.6%) were multigravida. Six (1.8%) of the women had a history of gestational diabetes, and four (1.2%) had hypertension. Only 4 (1.2%) were aware that bacteria could cause preterm labour. 11 (32%) visited the dentist during the period of their pregnancy. There was no amplification of 16SrRNA or the fadA genes in the tested samples. Conclusions: This study showed that preterm delivery was more common in multigravida pregnant women aged 31-35 years. Although some of the samples were cloudy and purulent, Fusobacterium nucleatum species-specific and virulent fadA genes were not detected in amniotic fluid obtained from the population of women with preterm labour studied.

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Section: Discussioncontrasting

confidence: 99%

Evaluation of Fusobacterium nucleatum in Amniotic Fluid of Women with Preterm Labour

Nwaokorie,

Okunade,

Edet

et al. 2023

Babcock Univ. Med. J.

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“…was observed in the urine of PTB women [ 59 ]. The BV strains Sneathia sanguinegens and Fusobacterium nucleatum were identified in amniotic fluid samples at mid-trimester of women with PTB [ 60 ]. The low levels of Clostridium subcluster (XVIII, XIVa) and Bacteroides and a high level of Lactobacillales were observed in the feces of the PTB [ 58 ].…”

Section: Microbiota Pathological Insight In Ptbmentioning

confidence: 99%

Molecular Mechanism of Microbiota Metabolites in Preterm Birth: Pathological and Therapeutic Insights

Ansari

Bose

You

et al. 2021

IJMS

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Preterm birth (PTB) refers to the birth of infants before 37 weeks of gestation and is a challenging issue worldwide. Evidence reveals that PTB is a multifactorial dysregulation mediated by a complex molecular mechanism. Thus, a better understanding of the complex molecular mechanisms underlying PTB is a prerequisite to explore effective therapeutic approaches. During early pregnancy, various physiological and metabolic changes occur as a result of endocrine and immune metabolism. The microbiota controls the physiological and metabolic mechanism of the host homeostasis, and dysbiosis of maternal microbial homeostasis dysregulates the mechanistic of fetal developmental processes and directly affects the birth outcome. Accumulating evidence indicates that metabolic dysregulation in the maternal or fetal membranes stimulates the inflammatory cytokines, which may positively progress the PTB. Although labour is regarded as an inflammatory process, it is still unclear how microbial dysbiosis could regulate the molecular mechanism of PTB. In this review based on recent research, we focused on both the pathological and therapeutic contribution of microbiota-generated metabolites to PTB and the possible molecular mechanisms.

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“…High-throughput, culture-independent technologies can be used to assess the microbiome of each organ in the body at high taxonomic resolution (Hillier et al, 1995; Hitti et al, 2001; Menon, 2008; Racicot et al, 2013). We previously reported analyses of the microbial communities of amniotic fluid and urine by sequencing of the bacterial 16S ribosomal RNA gene in women who delivered preterm (Yoo et al, 2016; You et al, 2016). We identified Sneathia sanguinegens and Fusobacterium nucleatum in amniotic fluid samples from two preterm delivered women (You et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…We previously reported analyses of the microbial communities of amniotic fluid and urine by sequencing of the bacterial 16S ribosomal RNA gene in women who delivered preterm (Yoo et al, 2016; You et al, 2016). We identified Sneathia sanguinegens and Fusobacterium nucleatum in amniotic fluid samples from two preterm delivered women (You et al, 2016). In urine samples, Ureaplasma spp.…”

Section: Introductionmentioning

confidence: 99%

“…and Veillonellaceae family members, including Megasphaera spp., were more abundant in preterm delivered women than in women who delivered at term (Yoo et al, 2016). While antibiotic use can alter the composition and structure of the microbiota in specific cases, such as in chorioamnionitis and cervical infection (You et al, 2016), microbes such as F. nucleatum , Leptotrichia ( Sneathia ), Ureaplasma urealyticum , Mycoplasma hominis , Streptococcus agalactiae , Escherichia coli , and a species of the order Clostridiales can induce local inflammatory responses in gestational tissues, causing PTB (Peltier, 2003; Han et al, 2006; Wang et al, 2013). However, the microbial composition of the blood of women who undergo preterm delivery has not been established.…”

Section: Introductionmentioning

confidence: 99%

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Blood Microbial Communities During Pregnancy Are Associated With Preterm Birth

et al. 2019

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Microbial infection of the placenta, amniotic fluid, vaginal canal, and oral cavity is known to significantly contribute to preterm birth (PTB). Although microbes can be translocated into the blood, little is known regarding the blood microbiota during pregnancy. To assess changes in the microbiome during pregnancy, blood samples were obtained 2 or 3 times during pregnancy from a cohort of 45 pregnant women enrolled between 2008 and 2010. To analyze the association with PTB, we conducted a case-control study involving 41 pregnant women upon admission for preterm labor and rupture of membrane (20 with term delivery; 21 with PTB). Bacterial diversity was assessed in number and composition between the first, second, and third trimesters in term delivered women according to 16S rRNA gene amplicon sequencing, and data were analyzed using Quantitative Insight Into Microbial Ecology (QIIME). Taxonomy was assigned using the GreenGenes 8.15.13 database. Dominant microorganisms at the phylum level in all pregnant women were identified as Firmicutes , Proteobacteria , Bacteroidetes , and Actinobacteria . However, the number and composition of bacteria in women with PTB differed from that in women with term delivery. Firmicutes and Bacteroidetes were more abundant in women with PTB than in women with term delivery, while Proteobacteria was less prevalent in women with PTB. At the genus level, Bacteroides , Lactobacillus , Sphingomonas , Fastidiosipila , Weissella , and Butyricicoccus were enriched in PTB samples. These observational results suggest that several taxa in the maternal blood microbiome are associated with PTB. Further studies are needed to confirm the composition of the blood microbiota in women with PTB. Additionally, the mechanism by which pathogenic microbes in maternal blood cause infection and PTB requires further analysis.

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New insight into the analysis of amniotic fluid microflora using 16S rRNA gene sequencing

Cited by 3 publications

References 29 publications

Evaluation of Fusobacterium nucleatum in Amniotic Fluid of Women with Preterm Labour

Evaluation of Fusobacterium nucleatum in Amniotic Fluid of Women with Preterm Labour

Molecular Mechanism of Microbiota Metabolites in Preterm Birth: Pathological and Therapeutic Insights

Blood Microbial Communities During Pregnancy Are Associated With Preterm Birth

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