New insight on the role of melatonin receptors in reproductive processes of seasonal breeders on the example of mature male European bison ( Bison bonasus , Linnaeus 1758)

Tabęcka-Łonczyńska, Anna; Mytych, Jennifer; Sołek, Przemysław; Kulpa, Magdalena; Koziorowski, Marek

doi:10.1016/j.jphotobiol.2017.05.026

Cited by 14 publications

(8 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The concentration of extracted total RNA was quantified using the NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific Inc., Waltham, MA, USA), and the RNA integrity was evaluated through the 1.0% agarose gel electrophoresis. Subsequently, cDNA was prepared from 1 μg of total RNA using High Capacity cDNA Reverse Transcription Kit as previously described (Tabecka-Lonczynska et al 2017). Afterwards, 1 ug RNA was diluted with sterile water and then mixed with 10 μl of master mix prepared freshly before use (2 μl of 10 × RT Buffer, 0.8 μl of 25 × dNTP Mix, 2 μl of 10 × RT Random Primers, 1 μl of Multiscribe Reverse Transcriptase, and 3.2 μl H 2 O).…”

Section: Rna Isolation Reverse Transcription and Rt Pcr Analysismentioning

confidence: 99%

“…In the European bison, the seasonality of reproduction results from cyclical changes in the structure and activity of the testis and the epididymis, and indicates the presence of molecular mechanisms responsible for the stimulation of cell proliferation and the induction of spermatogenesis in the reproductive period. These processes are strictly regulated not only by a normal function of the hypothalamic-pituitary-gonadal axis, but it also was demonstrated that local mechanisms have a great impact on them (McGuire et al 2011;Tabecka-Lonczynska et al 2018b;Tabecka-Lonczynska et al 2017). The normally functioning male reproductive system produces wholesome reproductive cells spermatozoa, with Leydig and Sertoli cells involved in this process.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Autophagy as a consequence of seasonal functions of testis and epididymis in adult male European bison (Bison bonasus, Linnaeus 1758)

et al. 2019

Self Cite

View full text Add to dashboard Cite

The European bison is still an animal endangered with extinction, so by learning factors that regulate its reproduction, we can contribute to the survival of this species. On the other hand, autophagy is a dynamic, lisosomal, and evolutionary conserved process which is essential for animal cell survival, homeostasis, and differentiation. This process was demonstrated in many species and in many organs; however, information on the metabolic course of autophagy in the male reproductive system in seasonally reproducing species is lacking. Therefore, in this study, we examined for the first time several autophagy-related factors (mTOR, ULK1, Atg13, PI3K, beclin1, beclin2, Atg14, Atg5, Atg16L, LC3) in testicular and epididymal tissues obtained from adult male individuals of the European bison. We compared the level of gene expression, protein synthesis, and localization of autophagy-related factors between June, September, and December (before, during, and after reproductive activity, respectively). We confirmed that the induction of autophagy was at the highest level in the period after reproductive activity, i.e., in December, when a significant increase in the gene and protein expression was observed for the majority of these factors, probably to ensure cellular protection. However, autophagy was also clearly marked in September, during the intense spermatogenesis, and this may indicate a great demand for autophagy-related proteins required for the normal development of reproductive cells. Obtained results seem to confirm that autophagy pathway, as a consequence of seasonal reproduction, may control the normal course of spermatogenesis in the male European bison.

show abstract

Section: Rna Isolation Reverse Transcription and Rt Pcr Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Autophagy as a consequence of seasonal functions of testis and epididymis in adult male European bison (Bison bonasus, Linnaeus 1758)

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition to its modulator activity on the HPG axis, melatonin is capable of passing through the blood-testis barrier, exerting a direct action on testicular tissue by binding its specific receptors 1 and 2 (MT1 and MT2) and regulating androgen production, increasing Sertoli cells responsiveness to FSH during testicular development, and modulating cellular growth and proliferation [12,15]. MT1 and MT2 are G protein-coupled receptors present in both Leydig and Sertoli cells in many animal species [10,[16][17][18][19][20]. In addition, melatonin can also be directly synthesized within the testis upon the action of two pivotal enzymes: aralkylamine-N-acetyltransferase (AANAT) and N-acetylserotonin-O-methyltransferase (ASMT), which catalyze the conversion of serotonin (5-HT) into melatonin [21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Testicular Melatonin and Its Pathway in Roe Deer Bucks (Capreolus capreolus) during Pre- and Post-Rut Periods: Correlation with Testicular Involution

Elmi

Govoni

Zannoni

et al. 2021

Animals

View full text Add to dashboard Cite

Roe deer are seasonal breeders with a complete yearly testicular cycle. The peak in reproductive activity is recorded during summer, the rutting period, with the highest levels of androgens and testicular weight. Melatonin plays a pivotal role in seasonal breeders by stimulating the hypothalamus–pituitary–gonads axis and acting locally; in different species, its synthesis within testes has been reported. The aim of this study was to evaluate the physiological melatonin pattern within roe deer testes by comparing data obtained from animals sampled during pre- and post-rut periods. Melatonin was quantified in testicular parenchyma, along with the genetic expression of enzymes involved in its local synthesis (AANAT and ASMT) and function (UCP1). Melatonin receptors, MT1-2, were quantified both at protein and gene expression levels. Finally, to assess changes in reproductive hormonal profiles, testicular dehydroepiandrosterone (DHEA) was quantified and used for a correlation analysis. Melatonin and AANAT were detected in all samples, without significant differences between pre- and post-rut periods. Despite DHEA levels confirming testicular involution during the post-rut period, no correlations appeared between such involution and melatonin pathways. This study represents the first report regarding melatonin synthesis in roe deer testes, opening the way for future prospective studies in the physiology of this species.

show abstract

“…Many reports have also shown that melatonin regulates Sertoli cells metabolism during gametes development. Melatonin, due to its amphiphilic properties, acts peripherally through binding to intracellular proteins, nuclear receptors and membrane melatonin receptors including MT1 and MT2 . In this regard, melatonin and melatonin receptors act via affecting the secretion of both gonadotropin‐releasing hormone and luteinizing hormone (LH) gonadotropins, and testosterone synthesis as well as testicular maturation.…”

Section: Introductionmentioning

confidence: 99%

The role of melatonin on chemotherapy-induced reproductive toxicity

Haghi‐Aminjan

Asghari

Farhood

et al. 2018

Journal of Pharmacy and Pharmacology

View full text Add to dashboard Cite

Objectives Reproductive malfunctions after chemotherapy still are a reason of reducing fertility and need specialized intensive care. The aim of this review was to investigate the effect of melatonin on the reproductive system under threatening with chemotherapeutic drugs. Methods To find the role of melatonin in the reproductive system during chemotherapy, a full systematic literature search was carried out based on Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines in the electronic databases up to 17 April 2017 using search terms in the titles and abstracts. A total of 380 articles are screened according to our inclusion and exclusion criteria. Finally, 18 articles were included in this study. Key findings It has been cleared that melatonin has bilateral effects on reproductive cells. Melatonin protects normal cells via mechanisms, including decrease in oxidative stress, apoptosis, inflammation and modulating mitochondrial function, and sexual hormones. Furthermore, melatonin with antiproliferative properties and direct effects on its receptors improves reproductive injury and function during chemotherapy. On the other hand, melatonin sensitizes the effects of chemotherapeutic drugs and enhances chemotherapy-induced toxicity in cancerous cells through increasing apoptosis, oxidative stress and mitochondrial malfunction. Conclusions The study provides evidence of the bilateral role of melatonin in the reproductive system during chemotherapy.

show abstract

New insight on the role of melatonin receptors in reproductive processes of seasonal breeders on the example of mature male European bison ( Bison bonasus , Linnaeus 1758)

Cited by 14 publications

References 48 publications

Autophagy as a consequence of seasonal functions of testis and epididymis in adult male European bison (Bison bonasus, Linnaeus 1758)

Autophagy as a consequence of seasonal functions of testis and epididymis in adult male European bison (Bison bonasus, Linnaeus 1758)

Testicular Melatonin and Its Pathway in Roe Deer Bucks (Capreolus capreolus) during Pre- and Post-Rut Periods: Correlation with Testicular Involution

The role of melatonin on chemotherapy-induced reproductive toxicity

Contact Info

Product

Resources

About