New insights on phage efficacy to control Aeromonas salmonicida in aquaculture systems: An in vitro preliminary study

Duarte, João; Pereira, Carla; Moreirinha, Catarina; Salvio, Rugiada; Lopes, Ana Isabel; Wang, Dapeng; Almeida, Adelaide

doi:10.1016/j.aquaculture.2018.07.002

Cited by 49 publications

(52 citation statements)

References 77 publications

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“…Also, using cocktails composed of phages targeting different receptors of the same bacteria can help slowing down the rate of resistance development (Tanji et al, 2004;Hall et al, 2012;Filippov et al, 2011;Crothers-Stomps et al, 2010). In aquaculture studies, resistance apparition rate to a cocktail typically stands between those of the individual phages and generally varies around 10 −4 and 10 −5 CFU/ml (Silva et al, 2014a;Duarte et al, 2018;Pereira et al, 2017;Moreirinha et al, 2018).…”

Section: Managing Host Specificity and Resistance With Phage Cocktailsmentioning

confidence: 99%

“…This could turn as a serious issue when designing a formulation for a phage cocktail (Malik et al, 2017;Leung et al, 2017;Leung et al, 2018). Peculiarities of the farming environment should also be taken into account as formulation may also be used to mitigate the impact of variables such as radiation and salinity (Duarte et al, 2018;Silva et al, 2014b). Apart from the fact that larger viruses are more sensitive to recrystallization in powder formulation, no clear correlation has yet be found between phage phylogeny and recommended formulation (Vandenheuvel et al, 2014).…”

Section: Stabilizing and Delivering Phages Thanks To Galenicsmentioning

confidence: 99%

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Overcoming the challenges of phage therapy for industrial aquaculture: A review

2019

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Aquaculture is the fastest-growing sector in food industry. Its development is powered by the intensification of the production which increased bacterial disease occurrence and spreading. As aquaculture deeply relies on a massive prophylactic and therapeutic use of antibiotics, it is threatened by the emergence of multi drug resistant bacteria. The stalled development of new antibiotics makes finding new therapeutic solutions a burning issue. Thanks to their specific host range, their ability to treat both the farmed species and the environment, their limited ecological impact and their abundance in the environment, bacteriophages represent a promising sustainable solution to control pathogenic aquaculture bacteria. In this review we discuss the interest of phage biocontrol for aquaculture and how can bacterial resistance, ecological, pharmacological and production related issues be solved.

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Section: Managing Host Specificity and Resistance With Phage Cocktailsmentioning

confidence: 99%

Section: Stabilizing and Delivering Phages Thanks To Galenicsmentioning

confidence: 99%

Overcoming the challenges of phage therapy for industrial aquaculture: A review

2019

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“…In in vitro studies, the use of effective phage cocktails (AS-A, AS-D, AS-E) against this pathogen showed a faster control of bacterial concentration as well as a decrease in the frequency of occurrence of phage-resistant mutants. [122]. Worthy of note is also the success obtained when the phage FCP1 was administered intramuscularly to treat the disease caused by F. columnaris in the catfish Clarias batrachus [126].…”

Section: Aquaculturementioning

confidence: 99%

A Hundred Years of Bacteriophages: Can Phages Replace Antibiotics in Agriculture and Aquaculture?

et al. 2020

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Agriculture, together with aquaculture, supplies most of the foodstuffs required by the world human population to survive. Hence, bacterial diseases affecting either agricultural crops, fish, or shellfish not only cause large economic losses to producers but can even create food shortages, resulting in malnutrition, or even famine, in vulnerable populations. Years of antibiotic use in the prevention and the treatment of these infections have greatly contributed to the emergence and the proliferation of multidrug-resistant bacteria. This review addresses the urgent need for alternative strategies for the use of antibiotics, focusing on the use of bacteriophages (phages) as biocontrol agents. Phages are viruses that specifically infect bacteria; they are highly host-specific and represent an environmentally-friendly alternative to antibiotics to control and kill pathogenic bacteria. The information evaluated here highlights the effectiveness of phages in the control of numerous major pathogens that affect both agriculture and aquaculture, with special emphasis on scientific and technological aspects still requiring further development to establish phagotherapy as a real universal alternative to antibiotic treatment.

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“…Nonetheless, phage inactivation can prove to be challenging following certain applications, such as crop treatment (Jones et al, 2012 ) or infection control in aquaculture (Richards, 2014 ). However, it must be noted that phage persistence in some environments is severely limited by UV-light inactivation, as is the case of the phyllosphere (Jones et al, 2012 ) or in aquaculture settings (Duarte et al, 2018 ). To overcome this limitation, some authors have recommended the application of bacteriophages at the end of the day or at night (Duarte et al, 2018 ).…”

mentioning

confidence: 99%

“…However, it must be noted that phage persistence in some environments is severely limited by UV-light inactivation, as is the case of the phyllosphere (Jones et al, 2012 ) or in aquaculture settings (Duarte et al, 2018 ). To overcome this limitation, some authors have recommended the application of bacteriophages at the end of the day or at night (Duarte et al, 2018 ). Overall, additional research is still required to examine not only the effectiveness of phages as antimicrobials, but also their persistence in the environment and the emergence of bacterial resistance in field trials.…”

mentioning

confidence: 99%