New Isomerization Technology for High Fructose Syrup Production

Abstract

A modern glucose isomerization system has been developed which differs from conventional immobilized systems which use isomerases such as crosslinked cell‐based products; the latter are discarded after enzyme inactivation. The newer system consists of two separate phases: an inert, regenerable carrier that has a high protein binding capacity, and an enzyme solution containing a pure isomerase protein. This system offers different possibilities for the immobilization: Batch immobilization loading the carrier fully with the enzyme. Batch immobilization loading the carrier only partly with the enzyme. Continuous or semi‐continuous loading of the carrier with enzyme during the isomerization process. The technique of a gradual addition of enzyme to the column during the isomerization process, which is called „on‐column loading”︁, is a flexible system providing several advantages compared to the traditional isomerization technology Initial flow rate according to current needs. Flexible capacity adjustable according to the syrup need of the markets. Few isomerization columns needed for stable and even operation. simple to operate. Uninterrupted operation for up to two years. Low isomerization costs. This technology has been successfully tested in plant scale in several HFS plants, and one plant has been totally designed to be operated with this technology. this plant has been in operation for more than 1.5 years with the full satisfaction of the user.

An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design

“…For instance, Antrim and his coworkers immobilized glucose isomerase (GI) from Streptomyces rubiginosus to DEAE-cellulose–polystyrene–TiO 2 resin using electrostatic binding, resulting in immobilized glucose isomerase (IGI) with catalytic densities of up to 1500 U g −1 . IGI is very stable, with a half-life of over 1800 h under recommended operating conditions at a pH range of 7.2–8.2 with a preferred range of 7.6–7.8, and a temperature range of 54–62 °C with the temperature of optimum productivity being about 57 °C [ 72 , 73 ]. Novozyme 435 (a lipase) is utilized to synthesize specialty esters industrially.…”

Diversidade genética em cultivares de mandioca (Manihot esculenta) da Região Amazônica, padrões de atividade amilolítica e expressão gênica de α-amilase

“…Além da -amilase e da glicoamilase, utilizadas na hidrólise principal do amido à glicose, outros tipos de amilases microbianas, como a pululanase, a isoamilase e a ciclodextrina-glicosiltransferase, são empregadas industrialmente na hidrólise seletiva do polissacarídeo, sendo obtidos produtos com características específicas e alguns com alto valor agregado, como as ciclodextrinas, insumos importantes na fabricação de alimentos, fármacos, cosméticos, e materiais termoplásticos biodegradáveis. O uso dessas enzimas possibilitou um avanço considerável na indústria do amido, em substituição à hidrólise ácida (ANTRIM et al, 1989).…”