New method for arbuscular mycorrhizal fungus spore separation using a microfluidic device based on manual temporary flow diversion

Srisom, K.; Tittabutr, Panlada; Teaumroong, Neung; Lapwong, Yingyod; Phatthanakun, Rungrueang; Sirivisoot, Sirinrath; Kuntanawat, Panwong

doi:10.1007/s00572-020-00986-4

Cited by 9 publications

(8 citation statements)

References 53 publications

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“… 35 However, only two single studies involving AMF have been published, which focus on spore sorting and extraradical hyphae. 36 …”

Section: Introductionmentioning

confidence: 99%

AMF-SporeChip provides new insights into arbuscular mycorrhizal fungal asymbiotic hyphal growth dynamics at the cellular level

Richter,

Calonne-Salmon,

van der Heijden

et al. 2024

Lab Chip

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“… 35 However, only two single studies involving AMF have been published, which focus on spore sorting and extraradical hyphae. 36 …”

Section: Introductionmentioning

confidence: 99%

AMF-SporeChip provides new insights into arbuscular mycorrhizal fungal asymbiotic hyphal growth dynamics at the cellular level

Richter,

Calonne-Salmon,

van der Heijden

et al. 2024

Lab Chip

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“…AMF spores are usually isolated by wet-sieving and centrifugation with a sucrose solution (WST) [ 22 , 23 ]. Some researchers have developed improved methods for spore extraction [ 24 , 25 , 26 , 27 , 28 , 29 ]. These methods mostly use a sieving step at 45 µm to extract AMF spores (50–750 µm and up to 1 mm) and exclude those of other fungi (2–10 µm range) [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, there is the presence of multiple unwanted substances that are extracted together. Removing these interferents requires the utilization of fine-diameter mesh filters and leads to the wastage of water [ 28 , 29 ]. Sugar-based solutions are employed to improve the purity of the spore suspension.…”

Section: Introductionmentioning

confidence: 99%

A New Technique for the Extraction of Arbuscular Mycorrhizae Fungal Spores from Rhizosphere

Boyno

Demir

Danesh

et al. 2023

JoF

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Monitoring the dynamics of the spore bank of arbuscular mycorrhizal fungi (AMF) is essential for the sustainable management and protection of agroecosystems. The most common method for extracting AMF spores from soil is the wet-sieving technique (WST). However, this method has many disadvantages. In this study, we modified the WST using new approaches: the ultrasound wet-sieving technique (UWST) and the ultrasound centrifuge technique (UCT). We enumerated and compared the numbers and quality of spores obtained from WST, UWST, and UCT to validate the new modified techniques. We extracted AMF spores from the rhizospheres of different plants, including wheat (Triticum aestivum L.), bean (Phaseolus vulgaris L.), tomato (Solanum lycopersicum L.), pepper (Piper nigrum L.), parsley (Petroselinum crispum Mill.), and turfgrass (Lolium perenne L.) collected from the Van Lake basin, Turkey. The highest and lowest AMF spore numbers were observed in wheat and turfgrass rhizospheres. The UCT allowed for the extraction of the highest number of spores from all rhizospheres, followed by the UWST and WST. The UWST and WST allowed for the extraction of similar spore numbers from wheat, pepper, parsley, and turfgrass rhizospheres. Beyond the high extracted spore number, UCT was shown to be a fast and low-material-consuming approach. These findings demonstrate that the UCT can be used to efficiently extract AMF spores in future research.

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“…Separation, purification, and enrichment of biological microparticles are necessary steps before clinical and bioresearch applications 1 . For example, circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) isolation help early-stage cancer diagnosis 2 – 4 , and spore enrichment is a prerequisite of chemical analysis and production 5 . Microfluidic separation devices have established their reputation based on a reduced sample and reagent volumes, improved portability, significant sensitivity, and low cost 6 .…”

Section: Introductionmentioning

confidence: 99%

“…Microfluidic separation devices have established their reputation based on a reduced sample and reagent volumes, improved portability, significant sensitivity, and low cost 6 . Minute bioparticles, such as red blood cells (RBCs) and white blood cells (WBCs) 7 , CTCs 2 , 8 – 10 , exosomes 11 , 12 , DNA 13 , parasites 14 , bacteria 15 , 16 , and spores 5 , 17 , 18 , can be separated by microfluidics based on their size differences and other attributes. There are two types of microfluidic separation devices: active and passive 19 .…”

Section: Introductionmentioning

confidence: 99%

Geometric structure design of passive label-free microfluidic systems for biological micro-object separation

et al. 2022

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Passive and label-free microfluidic devices have no complex external accessories or detection-interfering label particles. These devices are now widely used in medical and bioresearch applications, including cell focusing and cell separation. Geometric structure plays the most essential role when designing a passive and label-free microfluidic chip. An exquisitely designed geometric structure can change particle trajectories and improve chip performance. However, the geometric design principles of passive and label-free microfluidics have not been comprehensively acknowledged. Here, we review the geometric innovations of several microfluidic schemes, including deterministic lateral displacement (DLD), inertial microfluidics (IMF), and viscoelastic microfluidics (VEM), and summarize the most creative innovations and design principles of passive and label-free microfluidics. We aim to provide a guideline for researchers who have an interest in geometric innovations of passive label-free microfluidics.

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New method for arbuscular mycorrhizal fungus spore separation using a microfluidic device based on manual temporary flow diversion

Cited by 9 publications

References 53 publications

AMF-SporeChip provides new insights into arbuscular mycorrhizal fungal asymbiotic hyphal growth dynamics at the cellular level

AMF-SporeChip provides new insights into arbuscular mycorrhizal fungal asymbiotic hyphal growth dynamics at the cellular level

A New Technique for the Extraction of Arbuscular Mycorrhizae Fungal Spores from Rhizosphere

Geometric structure design of passive label-free microfluidic systems for biological micro-object separation

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