New method for quantification of gasotransmitter hydrogen sulfide in biological matrices by LC-MS/MS

Tan, Bo; Jin, Sheng; Sun, Junqiang; Gu, Zhongkai; Sun, Xiaotian; Zhu, Yi‐Chun; Huo, Keke; Cao, Zonglian; Yang, Ping; Xin, Xiaoming; Liu, Xinhua; Pan, Li-Long; Qiu, Furong; Jiang, Jian; Jia, Yanming; Ye, Fuyuan; Xie, Ying; Zhu, Yi Zhun

doi:10.1038/srep46278

Cited by 88 publications

(63 citation statements)

References 50 publications

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“…The level of free H 2 S in RBCs was 10.5±0.8 µmol l −1 in warm normoxic turtles ( Fig. 2A), which is not far from the value reported for human RBCs measured using a MBB-based method (3.8± 1.0 µmol l −1 ) (Tan et al, 2017). RBC free H 2 S increased 2-fold in cold-acclimated turtles in normoxia but did not change further in anoxia ( Fig.…”

Section: H 2 S and Bss In Rbcsmentioning

confidence: 49%

Tissue-dependent variations of hydrogen sulfide homeostasis in anoxic freshwater turtles

Jensen

Pardue

Kevil

et al. 2019

Journal of Experimental Biology

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Hydrogen sulfide (H 2 S) controls numerous physiological responses. To understand its proposed role in metabolic suppression, we measured free H 2 S and bound sulfane sulfur (BSS) in tissues of the freshwater turtle Trachemys scripta elegans, a species undergoing strong metabolic suppression when cold and anoxic. In warm normoxic turtles, free H 2 S was higher in red blood cells (RBCs) and kidney (∼9-10 µmol l −1 ) than in brain, liver and lung (∼1-2 µmol l −1 ). These values overall aligned with the tissue H 2 S-generating enzymatic activity. BSS levels were similar in all tissues (∼0.5 µmol l −1 ) but ∼100-fold higher in RBCs, which have a high thiol content, suggesting that RBCs function as a circulating H 2 S reservoir. Cold acclimation caused significant changes in free and bound H 2 S in liver, brain and RBCs, but anoxia had no further effect, except in the brain. These results show tissue-dependent sulfide signaling with a potential role in brain metabolic suppression during anoxia in turtles.

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Section: H 2 S and Bss In Rbcsmentioning

confidence: 49%

Tissue-dependent variations of hydrogen sulfide homeostasis in anoxic freshwater turtles

Jensen

Pardue

Kevil

et al. 2019

Journal of Experimental Biology

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“…Blood samples were collected in EDTA-containing tubes and tissues including heart, liver, kidney, spleen and lung removed. Samples were further processed according to previously reported methods and H 2 S levels determined as described by Tan et al 37 In brief, chromatography was performed using a Waters Acquity UPLC (Milford, MA, USA) equipped with a Kinetex XB-C18 column (50 mm × 2.1 mm, 2.6 μm) from Phenomenex (Torrance, CA, USA). The components were eluted by a gradient of A) 0.08% formic acid in water and B) acetonitrile and the flow rate was 0.6 mL/min.…”

Section: Pharmacokinetic Studiesmentioning

confidence: 99%

<p>A Novel Liposomal S-Propargyl-Cysteine: A Sustained Release of Hydrogen Sulfide Reducing Myocardial Fibrosis via TGF-β1/Smad Pathway</p>

Tran

Chang

et al. 2019

IJN

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S-propargyl-cysteine (SPRC; alternatively known as ZYZ-802) is a novel modulator of endogenous tissue H 2 S concentrations with known cardioprotective and antiinflammatory effects. However, its rapid metabolism and excretion have limited its clinical application. To overcome these issues, we have developed some novel liposomal carriers to deliver ZYZ-802 to cells and tissues and have characterized their physicochemical, morphological and pharmacological properties. Methods: Two liposomal formulations of ZYZ-802 were prepared by thin-layer hydration and the morphological characteristics of each liposome system were assessed using a laser particle size analyzer and transmission electron microscopy. The entrapment efficiency and ZYZ-802 release profiles were determined following ultrafiltration centrifugation, dialysis tube and HPLC measurements. LC-MS/MS was used to evaluate the pharmacokinetic parameters and tissue distribution profiles of each formulation via the measurements of plasma and tissues ZYZ-802 and H 2 S concentrations. Using an in vivo model of heart failure (HF), the cardio-protective effects of liposomal carrier were determined by echocardiography, histopathology, Western blot and the assessment of antioxidant and myocardial fibrosis markers. Results: Both liposomal formulations improved ZYZ-802 pharmacokinetics and optimized H 2 S concentrations in plasma and tissues. Liposomal ZYZ-802 showed enhanced cardioprotective effects in vivo. Importantly, liposomal ZYZ-802 could inhibit myocardial fibrosis via the inhibition of the TGF-β1/Smad signaling pathway. Conclusion: The liposomal formulations of ZYZ-802 have enhanced pharmacokinetic and pharmacological properties in vivo. This work is the first report to describe the development of liposomal formulations to improve the sustained release of H 2 S within tissues.

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“…The blood collected was anticoagulated with heparin sodium (50 U/mL) and centrifuged (3000 rpm, 15 min) to obtain plasma. As to the H 2 S concentration of DATS@MION-PEG-LF in plasma cannot be assessed by real time H 2 Sselective microelectrode assay, therefore it was measured by HPLC method by an Agilent Technologies HPLC (1260 in nity, CA, USA) with uorescence detection (λex: 390 nm and λem: 475 nm) and an Eclipse XDB-C18 column (150 ⋅ 4.6 mm, 5 µm) within 24 h, which was previously described [7,29].…”

Section: In Vivo H 2 S Release Of Dats@mion-peg-lfmentioning

confidence: 99%

Novel Controlled and Targeted Releasing Hydrogen Sulfide System Exerts Combinational Cerebral and Myocardial Protection after Cardiac Arrest

Sun

Wang

Wen

et al. 2020

Preprint

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Background : Cardiac arrest (CA) is a leading cause of death worldwide. Even after successful cardiopulmonary resuscitation (CPR), the majorities of survivals are companied with permanent myocardial and cerebral injury. Hydrogen sulfide (H 2 S) has been recognized as a novel gasotransmitter exerting multiple organ protection; however, the lacks of ideal H 2 S donors which can controlled release H 2 S to targeted organs such as heart and brain limits its application. Results : This work utilized mesoporous iron oxide nanoparticle (MION) as the carriers of diallyl trisulfide (DATS), with polyethylene glycol (PEG) and lactoferrin (LF) modified to MIONs to acquire the prolonged circulation time and brain-targeting effects, and a novel targeted H 2 S releasing system was constructed ( DATS@MION-PEG-LF ), which exhibited excellent biocompatibility, controlled-releasing H 2 S pattern, heart and brain targeting features, and the ability to be non-invasive traced by magnetic resonance imaging. DATS@MION-PEG-LF presented potent protective effects against cerebral and cardiac ischemic injury after CA in both in vitro hypoxia/reoxygenation models and in vivo CA/CPR models, which mainly involves anti-apoptosis, anti-inflammatory and anti-oxidant mechanisms. Accordingly, the cardiac and cerebral functions were obviously improved after CA/CPR, with potentially improved survival. Conclusions : The present work provides a unique platform for targeted controlled release of H 2 S based on MIONs, and offers a new method for combinational myocardial and cerebral protection from ischemic injury, bringing considerable benefits for CA patients.

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New method for quantification of gasotransmitter hydrogen sulfide in biological matrices by LC-MS/MS

Cited by 88 publications

References 50 publications

Tissue-dependent variations of hydrogen sulfide homeostasis in anoxic freshwater turtles

Tissue-dependent variations of hydrogen sulfide homeostasis in anoxic freshwater turtles

<p>A Novel Liposomal S-Propargyl-Cysteine: A Sustained Release of Hydrogen Sulfide Reducing Myocardial Fibrosis via TGF-β1/Smad Pathway</p>

Novel Controlled and Targeted Releasing Hydrogen Sulfide System Exerts Combinational Cerebral and Myocardial Protection after Cardiac Arrest

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