New method of evaluating sperm morphology with predictive value for human in vitro fertilization

Kruger, Thinus F.; Acosta, Anı́bal A.; Simmons, Kathryn; Swanson, R. James; Matta, James F.; Veeck, Lucinda L.; Morshedi, Mahmood; Brugo, S.

doi:10.1016/0090-4295(87)90246-9

Cited by 252 publications

(155 citation statements)

References 7 publications

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“…It is generally accepted that good-quality human MII oocytes should have a clear, moderately granular cytoplasm that does not contain inclusions, a small perivitelline space (PVS) containing a single unfragmented IPB and a round, clear, colourless zona pellucida (ZP) [29,44,45]. Nevertheless, more than half of all collected oocytes show at least one morphological abnormality [19,21].…”

Section: Introductionmentioning

confidence: 99%

The predictive value of high-magnification sperm morphology examination on ICSI outcomes in the presence of oocyte dysmorphisms

Setti

Braga

Figueira

et al. 2012

J Assist Reprod Genet

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Purpose To evaluate the relationship between oocyte dysmorphisms and IMSI outcomes Methods Data of IMSI cycles performed in 332 patients were included in this study. Patients were included only if presented more than four and less than 30 oocytes upon oocyte retrieval. Patients who underwent IMSI were matched, concerning female age and sperm parameters, with patients who underwent ICSI in the same period (n0332). The two groups (ICSI and IMSI) were compared with regard to treatment outcomes. The influence of IMSI on fertilization and embryo quality on D3 and D5, when oocyte dysmorphisms were present was analyzed. Results A total of 6444 oocytes were morphologically evaluated and injected. Regardless of the oocyte quality, IMSI performance was a determinant of the increased odds of development to high quality embryo on D3 (OR: 1.98; CI: 1.54-2.56) and D5 (OR: 3.27; CI: 1.61-6.66). Conclusions The selection of a morphologically normal spermatozoon under high-magnification is associated with increased embryo quality, regardless of the oocyte morphology. The injection of a spermatozoon, selected under highmagnification, into a morphologically normal oocyte leads to the highest probability of developing high quality embryos.

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Section: Introductionmentioning

confidence: 99%

The predictive value of high-magnification sperm morphology examination on ICSI outcomes in the presence of oocyte dysmorphisms

Setti

Braga

Figueira

et al. 2012

J Assist Reprod Genet

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“…Basically, an aliquot from each semen specimen was analyzed and sperm kinematic parameters measured using the Hamilton-Thorn HTM-C (HamiltonThorn, Danvers, MA) computer CASA system [10]. Sperm strict normal morphology and acrosome intactness were determined using the Tygerberg strict criteria method [5,6] and the Spermac method [11] respectively and described below.…”

Section: Preparation and Analyses Of Spermmentioning

confidence: 99%

“…The Tygerberg strict criteria method [5,6] was used to analyze the morphology of at least 100 sperm. A sperm was classified as normal when the head was oval with the acrosome occupying 40-70% of the head, absence of midpiece and tail defects and absent or small cytoplasmic droplets with the appropriate head dimensions.…”

Section: Preparation and Analyses Of Spermmentioning

confidence: 99%

“…Currently, sperm is randomly selected for intracytoplasmic sperm injection (ICSI) based on a reasonable shape, motility without consideration for strict normal morphology [2]. Increasing the percentage of sperm with strict normal morphology is associated with an increase in sperm with normal DNA [3,4] and enhances fertilization and pregnancy outcomes [5][6][7][8]. A simple method to select for cryopreserved-thawed sperm with normal strict morphology based on membrane charge is proposed here.…”

Section: Introductionmentioning

confidence: 99%

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Retention of membrane charge attributes by cryopreserved-thawed sperm and zeta selection

Kam

Jacobson

Patton

et al. 2007

J Assist Reprod Genet

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Purpose Mature sperm can be selected based on their negative zeta electrokinetic potential. The zeta selection of cryopreserved sperm is unknown. The objective was to study the effect of zeta processing on the morphology and kinematic parameters of cryopreserved-thawed sperm. Methods Colloid-washed sperm (N=9 cases) were cryopreserved for 24 h, thawed and diluted in serum-free medium in positive-charged tubes. After centrifugation, the tubes were decanted, serum-supplemented medium was added and the resuspended sperm were analyzed. Untreated sperm and fresh sperm served as the controls. Results There were improvements in strict normal morphology in fresh (11.8±0.3 versus control 8.8±0.3 %, mean ±SEM) and thawed (8.7±0.2 versus control 5.4±0.2%) sperm after zeta processing. Percent sperm necrosis was reduced after zeta processing (66.0±0.6 versus unprocessed 74.6±0.3%). Progression decreased by 50% but not total motility after zeta processing of thawed sperm. Conclusions The results suggested that the cryopreservation process did not impact the sperm membrane net zeta potential and higher percentages of sperm with normal strict morphology, acrosome integrity and reduced necrosis were recovered. The zeta method was simple and improved the selection of quality sperm after cryopreservation but more studies would be needed before routine clinical application.

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“…It is believed that sperm morphology may correlate with a man's fertility potential as reflected by in vitro fertilization (IVF) success in case cohort studies. 7 More recently, however, the ability of sperm morphology to distinguish candidates who are at risk of failing IVF has been called into question. 8 In addition, test reliability and reproducibility are low and normal biological variation may be high, all of which complicate and reduce the performance of sperm morphology as a diagnostic test.…”

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confidence: 99%

An Assessment of New Sperm Tests for Male Infertility

Natali

Turek

2011

Urology

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The routine semen analysis, although used for more than 50 years, fails to accurately distinguish between fertile and infertile men. As a consequence, many tests of sperm function (TSF) have been developed. This review discusses both older and newer diagnostic TSF. It outlines the principles underlying each assay and reviews aggregate clinical data to determine its current relevance and utility. It concludes that the relevance of many older TSF is questionable, with the wide acceptance of intracytoplasmic sperm injection (ICSI). Newer TSF have the potential to deliver more clinically relevant information but require more extensive study to better understand their predictive role in the ICSI era. UROLOGY 77: 1027-1034, 2011. © 2011 Elsevier Inc. All rights reserved.I n addition to the medical history and physical examination, the conventional semen analysis has been an essential laboratory test for the evaluation of male fertility for at least 50 years. However, the idea that fertility can be defined by threshold values of semen parameters is a concept that is fundamentally flawed. 1 Although not a true measure of fertility, the semen analysis, if abnormal, suggests that the probability of achieving fertility is lower than normal. 2 Because of biological variability, two semen analyses are generally needed, performed with two to three days of sexual abstinence and evaluated in a standardized fashion. 2 Recognized normal values for semen parameters are given in Table 1 in agreement with the latest recommendations by the World Health Organization (WHO). 2 Traditionally, (except for the latest WHO recommendations), these references are derived by expert consensus and not by prospective clinical trials and thus their true relationship to male fertility is unclear. In addition, the definition of what constitutes "normal" semen parameters is constantly challenged. A meta-analysis of 29 US studies of semen quality from 9612 fertile, or presumably fertile, men suggested that a sperm concentration of 98 million/mL is normal. 3 Although sperm motility is considered the "best" predictor of fertility, normal sperm motility ranges from 53% to 62%. 4 Thus, simply deriving "normal" semen parameters has been a prohibitively lengthy and inconclusive process to date.Finally, other biological variables affect the clinical utility of the semen analysis. In addition to wide intraindividual variation, seasonal 5 and geographic variation 4 further complicate the performance of the semen analysis as a fertility measure. As examples, within-subject, interejaculate coefficients of variation for sperm concentration and motility are estimated at 44.7% and 15% in one study. 1 Thus, even with excellent quality control, wide biological variation in semen quality profoundly challenges the notion that the conventional semen analysis can accurately assess male fertility. OLDER ADJUNCTIVE SPERM TESTSBecause of the need to more precisely characterize normal fertile semen, adjunctive semen testing has become popular (Figure 1). The concept behind de...

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New method of evaluating sperm morphology with predictive value for human in vitro fertilization

Cited by 252 publications

References 7 publications

The predictive value of high-magnification sperm morphology examination on ICSI outcomes in the presence of oocyte dysmorphisms

The predictive value of high-magnification sperm morphology examination on ICSI outcomes in the presence of oocyte dysmorphisms

Retention of membrane charge attributes by cryopreserved-thawed sperm and zeta selection

An Assessment of New Sperm Tests for Male Infertility

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