New Method Showing the Influence of Matrix Components in Leuconostoc mesenteroides Biofilm Formation

Badel, S.; Laroche, Céline; Gardarin, Christine; Bernardi, Thierry; Michaud, Philippe

doi:10.1007/s12010-008-8199-y

Cited by 30 publications

(15 citation statements)

References 14 publications

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“…The Biofilm Ring Test was further developed to test the sensitivity to antibiotics of clinical isolates in biofilm as "the antibiofilmogram", which provides complementary and sometimes more relevant information compared to a classical antibiogram. The Biofilm Ring Test has also been extended to study the contribution of different molecular determinants to the mechanisms of biofilm formation by different bacterial species (Badel et al, 2008(Badel et al, , 2011. Like the microtiter plate, the Biofilm Ring Test is sensitive to sedimentation due to gravity.…”

Section: Microtiter Platesmentioning

confidence: 99%

Critical review on biofilm methods

Azeredo

Azevedo

Briandet

et al. 2016

Critical Reviews in Microbiology

786

679

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Biofilms are widespread in nature and constitute an important strategy implemented by microorganisms to survive in sometimes harsh environmental conditions. They can be beneficial or have a negative impact particularly when formed in industrial settings or on medical devices. As such, research into the formation and elimination of biofilms is important for many disciplines. Several new methodologies have been recently developed for, or adapted to, biofilm studies that have contributed to deeper knowledge on biofilm physiology, structure and composition. In this review, traditional and cutting-edge methods to study biofilm biomass, viability, structure, composition and physiology are addressed. Moreover, as there is a lack of consensus among the diversity of techniques used to grow and study biofilms. This review intends to remedy this, by giving a critical perspective, highlighting the advantages and limitations of several methods. Accordingly, this review aims at helping scientists in finding the most appropriate and up-to-date methods to study their biofilms. ARTICLE HISTORY

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Section: Microtiter Platesmentioning

confidence: 99%

Critical review on biofilm methods

Azeredo

Azevedo

Briandet

et al. 2016

Critical Reviews in Microbiology

786

679

View full text Add to dashboard Cite

show abstract

“…This is generally used to study the kinetics of biofilm formation of Listeria monocytogenes, E. coli, Staphylococcus carnosus and Staphylococcus xylosus, 143 to measure the biofilm matrix mechanisms on Leuconostoc mesenteroides biofilm formation. 144 It can also be used to assess the antibiotics' effects on P. aeruginosa biofilms, 145 to relate the formation of biofilm among Campylobacter coli and Campylobacter jejuni. 146 Flow displacement method.…”

Section: Treatment Of Medical Implant-associated Infections With Nanomentioning

confidence: 99%

Bacterial adherence and biofilm formation on medical implants: A review

Veerachamy

Yarlagadda

Manivasagam

et al. 2014

Proc Inst Mech Eng H

453

347

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Biofilms are a complex group of microbial cells that adhere to the exopolysaccharide matrix present on the surface of medical devices. Biofilm-associated infections in the medical devices pose a serious problem to the public health and adversely affect the function of the device. Medical implants used in oral and orthopedic surgery are fabricated using alloys such as stainless steel and titanium. The biological behavior, such as osseointegration and its antibacterial activity, essentially depends on both the chemical composition and the morphology of the surface of the device. Surface treatment of medical implants by various physical and chemical techniques are attempted in order to improve their surface properties so as to facilitate bio-integration and prevent bacterial adhesion. The potential source of infection of the surrounding tissue and antimicrobial strategies are from bacteria adherent to or in a biofilm on the implant which should prevent both biofilm formation and tissue colonization. This article provides an overview of bacterial biofilm formation and methods adopted for the inhibition of bacterial adhesion on medical implants.

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“…Fogler (1999, 2000) concluded that dextran was important for biofilm formation of one strain of L. mesenteroides in porous medium. Badel et al (2008) found that dextran, proteins, and nucleic acids were all implied in biofilm formation by another strain of L. mesenteroides. On the other hand, some studies suggest that polysaccharides may not always be essential for biofilm formation (Jahn and Nielsen 1998;Wozniak et al 2003).…”

Section: Introductionmentioning

confidence: 96%

Biofilm formation by strains of Leuconostoc citreum and L. mesenteroides

Leathers

Bischoff

2010

Biotechnol Lett

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Although biofilms produced by various Leuconostoc sp. are economically important as contaminants of sugar processing plants, very few studies are available on these systems. Twelve strains of Leuconostoc citreum and L. mesenteroides that produce a variety of extracellular glucans were compared for their capacity to produce biofilms. 16s rRNA sequence analysis was used to confirm the species identity of these strains, which included four isolates of L. mesenteroides, five isolates of L. citreum, and three glucansucrase mutants of L. citreum strain NRRL B-1355. Strains identified as L. mesenteroides produce glucans that are generally similar to commercial dextran. Nevertheless, these strains differed widely in their capacity to form biofilms, with densities ranging from 2.7 to 6.1 log cfu/cm(2). L. citreum strains and their derivatives produce a variety of glucans. These strains exhibited biofilm densities ranging from 2.5 to 5.9 log cfu/cm(2). Thus, biofilm-forming capacity varied widely on a strain-specific basis in both species. The types of polysaccharides produced did not appear to affect the ability to form biofilms.

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New Method Showing the Influence of Matrix Components in Leuconostoc mesenteroides Biofilm Formation

Cited by 30 publications

References 14 publications

Critical review on biofilm methods

Critical review on biofilm methods

Bacterial adherence and biofilm formation on medical implants: A review

Biofilm formation by strains of Leuconostoc citreum and L. mesenteroides

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