New Methodology for Diagnosing Nitrogen Nutrition and Predicting Wheat Grain Quality

Новиков, Н Н

doi:10.26897/0021-342x-2017-5-29-40

Cited by 3 publications

(2 citation statements)

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“…Полученный сок приливали в пробирки с 3%-ным раствором трихлоруксусной кислоты для осаждения белков и связанных с ними пигментов. затем кислоту нейтрализовали гидроксидом натрия и измеряли оптическую плотность полученного раствора при длине волны 280 нм, по которой оценивали содержание аминокислот в соке листьев в пересчете на тирозин (мг тирозина в 1 мл сока) [7].…”

Section: методика исследованийunclassified

“…В опытах с разными дозами азота было установлено, что концентрация аминокислот в соке листьев пшеницы и ячменя повышалась при низком уровне азотного питания растений. При увеличении доз азота до оптимального уровня содержание аминокислот в соке листьев понижалось вследствие активизации их использования на синтез структурных и функционально активных белков, нуклеотидов и других азотистых веществ, что было обусловлено усилением ростовых процессов в результате улучшения питания растений азотом [7,9].…”

Section: Introductionunclassified

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Nutrient Diagnostics and Biochemical Indicators of Oat Grain Quality Predicted by Leaf Juice Amino Acid Concentration

Novikov,

Sokolov,

Tereshenkov

2023

Тимирязевский биологический журнал

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In order to find out the relationship between the level of nitrogen nutrition of oat plants of the Yakov variety and the content of amino acids in leaf juice, as well as the possibility of using this indicator to assess the content and composition of proteins, the activity of amylases, catalases and peroxidases in ripe grain and the activity of theseenzymes in germinating grain, a field experiment was carried out at the Field Experimental Station of Russian State Agrarian University – Moscow Timiryazev Agricultural Academy, on sod-podzolic medium loamy soil. In the field experiment, variants with different levels of nitrogen nutrition of oat plants that had passed post-harvest ripening were tudied. Ammonium nitrate, double superphosphate, and potassium chloride were applied in advance. As a result, it was found that as the level of nitrogen nutrition of plants increased, the concentration of amino acids in the juice of oat leaves at the stage of formation of the first stem node decreased, which was closely correlated with the nitrogen doses. These data indicate the possibility of using this indicator for the diagnosis of nitrogen nutrition in oat plants. The studies also revealed a reliable correlation between the content of amino acids in leaf juice and the concentration of proteins soluble in water and solutions of 10% KCl, 0.2% NaOH, as well as the activity of acid (pH 5.5), neutral (pH 7) and alkaline (pH 8) isoenzymes of α-amylase and acid isoforms of β-amylase in ripe grain. In addition, the amino acid content of leaf juice was found to be significantly correlated with the activity of acidic α-amylase, acidic and neutral β-amylase in germinated grain. The results obtained demonstrate the possibility of predicting the level of protein accumulation in oat grains. It is also possible to predict the protein composition, activity of α- and β-amylase, catalase, and peroxidase isoenzymes from the content of amino acids in leaf juice in the phase of the first stem node formation. A reliable correlation was also found for the activity of acid and neutral isoenzymes of α-amylase, catalase, and peroxidase in ripe and germinated oat grain.

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Section: методика исследованийunclassified

Section: Introductionunclassified

Nutrient Diagnostics and Biochemical Indicators of Oat Grain Quality Predicted by Leaf Juice Amino Acid Concentration

Novikov,

Sokolov,

Tereshenkov

2023

Тимирязевский биологический журнал

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Biological Rationale for the Protein Growth Stimulant Application to Increase Wheat Yield and Assessment of Grain Quality Using Microfocus X-ray and Optical Imaging Techniques

Колесников

Kremenevskaya

Priyatkin

et al. 2020

Russ. Agricult. Sci.

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Variation of winter wheat grain quality traits in the Steppe zone of the Rostov region

Fomenko,

Oleynikova,

Babrovskaya

et al. 2024

Vestnik of the Russian agricultural science

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The research was carried out in 2016–2023 in the steppe zone of the Rostov region. The article presents data on the variability of grain quality indicators in various environmental conditions in varieties of winter soft wheat of the selection Federal Rostov Agricultural Research Centre: Donskaya T 20, Bogema, Pafos, Palmira 18, Donya, Konstanta 22. The soil of the experimental site is southern medium – sized carbonate chernozem. The predecessor in the experiment is black steam. Various correlations from r = –0,74–0,26 between yield and protein content in grain were revealed. The ratio of the gluten and protein content of winter wheat in the steppe zone of the Rostov region is 1,3–2,3. In the conditions of the steppe of the Rostov region, the high stability of the varieties of winter wheat of the French selection was noted in terms of protein content, gluten, volume yield of bread and grain vitreousness. The greatest variability was revealed on the basis of the number of drops that determines the activity of α-amylase, which indicates the dependence of the genotype on the conditions for the formation of grain quality. The influence of proteolytic enzymes of the bug harmful turtle (Eurygaster integriceps) on the technological and baking properties of varieties was revealed.

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New Methodology for Diagnosing Nitrogen Nutrition and Predicting Wheat Grain Quality

Cited by 3 publications

References 0 publications

Nutrient Diagnostics and Biochemical Indicators of Oat Grain Quality Predicted by Leaf Juice Amino Acid Concentration

Nutrient Diagnostics and Biochemical Indicators of Oat Grain Quality Predicted by Leaf Juice Amino Acid Concentration

Biological Rationale for the Protein Growth Stimulant Application to Increase Wheat Yield and Assessment of Grain Quality Using Microfocus X-ray and Optical Imaging Techniques

Variation of winter wheat grain quality traits in the Steppe zone of the Rostov region

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