New microsatellite markers developed from an enriched microsatellite common bean library

Cardoso, Juliana Morini Kupper; Oblessuc, Paula Rodrigues; Campos, Tatiana de; Sforça, Danilo Augusto; Carbonell, Sérgio Augusto Moraes; Chioratto, Alisson Fernando; Formighieri, Eduardo Fernandes; Souza, Anete Pereira de; Benchimol, Luciana Lasry

doi:10.1590/s0100-204x2008000700019

Cited by 12 publications

(13 citation statements)

References 14 publications

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“…A parental polymorphism survey involving 220 Simple Sequence Repeat (SSR) markers spanning all eleven chromosomes was carried out, and polymorphic loci were used for the construction of the genetic linkage map. SSR markers were named according to the respective authors (IAC-, [ 107 , 108 , 109 ]; BM-, GATS-, [ 110 , 111 ]; BMb-, [ 112 ]; BMc-, [ 113 , 114 ]; BMd-, [ 115 ]; PVBR-, [ 116 , 117 ]; PVEST-, [ 98 ]; PvM-, [ 118 ]). PCR amplifications were performed according to author’s instructions with some modifications.…”

Section: Methodsmentioning

confidence: 99%

Dissection of Resistance Genes to Pseudomonas syringae pv. phaseolicola in UI3 Common Bean Cultivar

González

Godoy

Santalla

2017

IJMS

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Few quantitative trait loci have been mapped for resistance to Pseudomonas syringae pv. phaseolicola in common bean. Two F2 populations were developed from the host differential UI3 cultivar. The objective of this study was to further characterize the resistance to races 1, 5, 7 and 9 of Psp included in UI3. Using a QTL mapping approach, 16 and 11 main-effect QTLs for pod and primary leaf resistance were located on LG10, explaining up to 90% and 26% of the phenotypic variation, respectively. The homologous genomic region corresponding to primary leaf resistance QTLs detected tested positive for the presence of resistance-associated gene cluster encoding nucleotide-binding and leucine-rich repeat (NL), Natural Resistance Associated Macrophage (NRAMP) and Pentatricopeptide Repeat family (PPR) proteins. It is worth noting that the main effect QTLs for resistance in pod were located inside a 3.5 Mb genomic region that included the Phvul.010G021200 gene, which encodes a protein that has the highest sequence similarity to the RIN4 gene of Arabidopsis, and can be considered an important candidate gene for the organ-specific QTLs identified here. These results support that resistance to Psp from UI3 might result from the immune response activated by combinations of R proteins, and suggest the guard model as an important mechanism in pod resistance to halo blight. The candidate genes identified here warrant functional studies that will help in characterizing the actual defense gene(s) in UI3 genotype.

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Section: Methodsmentioning

confidence: 99%

Dissection of Resistance Genes to Pseudomonas syringae pv. phaseolicola in UI3 Common Bean Cultivar

González

Godoy

Santalla

2017

IJMS

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“…SSR markers were named according to the respective authors [IAC: Benchimol et al, 2007; Cardoso et al, 2008; Campos et al, 2011; BM, GATS: Gaitán-Solís et al, 2002; Blair et al, 2009a; BMb: Córdoba et al, 2010; BMc: Blair et al, 2009b, 2011; BMd: Blair et al, 2003; PV, Pvtttc001: Yu et al, 2000; PvBR: Buso et al, 2006; Grisi et al, 2007; PVEST, X04001: García et al, 2011; PvM: Hanai et al, 2007]. SSR markers were evaluated either by gel electrophoresis or capillary electrophoresis in an ABI PRISM® 3130 XL Genetic Analyzer (Applied Biosystems, USA).…”

Section: Methodsmentioning

confidence: 99%

Major Contribution of Flowering Time and Vegetative Growth to Plant Production in Common Bean As Deduced from a Comparative Genetic Mapping

González

Yuste‐Lisbona

Saburido³

et al. 2016

Front. Plant Sci.

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Determinacy growth habit and accelerated flowering traits were selected during or after domestication in common bean. Both processes affect several presumed adaptive traits such as the rate of plant production. There is a close association between flowering initiation and vegetative growth; however, interactions among these two crucial developmental processes and their genetic bases remain unexplored. In this study, with the aim to establish the genetic relationships between these complex processes, a multi-environment quantitative trait locus (QTL) mapping approach was performed in two recombinant inbred line populations derived from inter-gene pool crosses between determinate and indeterminate genotypes. Additive and epistatic QTLs were found to regulate flowering time, vegetative growth, and rate of plant production. Moreover, the pleiotropic patterns of the identified QTLs evidenced that regions controlling time to flowering traits, directly or indirectly, are also involved in the regulation of plant production traits. Further QTL analysis highlighted one QTL, on the lower arm of the linkage group Pv01, harboring the Phvul.001G189200 gene, homologous to the Arabidopsis thaliana TERMINAL FLOWER1 (TFL1) gene, which explained up to 32% of phenotypic variation for time to flowering, 66% for vegetative growth, and 19% for rate of plant production. This finding was consistent with previous results, which have also suggested Phvul.001G189200 (PvTFL1y) as a candidate gene for determinacy locus. The information here reported can also be applied in breeding programs seeking to optimize key agronomic traits, such as time to flowering, plant height and an improved reproductive biomass, pods, and seed size, as well as yield.

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“…Different sets of SSR and SNP markers previously reported (references are shown below) were tested for polymorphism in the parental genotypes, and polymorphic loci were used for the construction of the genetic linkage map. SSR markers were named according to the respective authors (IAC-, [ 49 , 62 , 63 ]; ATA-, [ 45 ]; BM-, GATS-, [ 64 , 65 ]; BMb-, [ 47 ]; BMc-, [ 46 , 52 ]; BMd-, [ 66 ]; PV-, [ 67 ]; PVBR-, [ 68 , 69 ]; PVEST-, [ 50 ]; PvM-, FJ-, [ 26 , 70 ]). PCR reactions were carried out following the protocols described in the publications mentioned above, although PCR conditions were changed for some SSR markers.…”

Section: Methodsmentioning

confidence: 99%

Marker-based linkage map of Andean common bean (Phaseolus vulgarisL.) and mapping of QTLs underlying popping ability traits

et al. 2012

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BackgroundNuña bean is a type of ancient common bean (Phaseolus vulgaris L.) native to the Andean region of South America, whose seeds possess the unusual property of popping. The nutritional features of popped seeds make them a healthy low fat and high protein snack. However, flowering of nuña bean only takes place under short-day photoperiod conditions, which means a difficulty to extend production to areas where such conditions do not prevail. Therefore, breeding programs of adaptation traits will facilitate the diversification of the bean crops and the development of new varieties with enhanced healthy properties. Although the popping trait has been profusely studied in maize (popcorn), little is known about the biology and genetic basis of the popping ability in common bean. To obtain insights into the genetics of popping ability related traits of nuña bean, a comprehensive quantitative trait loci (QTL) analysis was performed to detect single-locus and epistatic QTLs responsible for the phenotypic variance observed in these traits.ResultsA mapping population of 185 recombinant inbred lines (RILs) derived from a cross between two Andean common bean genotypes was evaluated for three popping related traits, popping dimension index (PDI), expansion coefficient (EC), and percentage of unpopped seeds (PUS), in five different environmental conditions. The genetic map constructed included 193 loci across 12 linkage groups (LGs), covering a genetic distance of 822.1 cM, with an average of 4.3 cM per marker. Individual and multi-environment QTL analyses detected a total of nineteen single-locus QTLs, highlighting among them the co-localized QTLs for the three popping ability traits placed on LGs 3, 5, 6, and 7, which together explained 24.9, 14.5, and 25.3% of the phenotypic variance for PDI, EC, and PUS, respectively. Interestingly, epistatic interactions among QTLs have been detected, which could have a key role in the genetic control of popping.ConclusionsThe QTLs here reported constitute useful tools for marker assisted selection breeding programs aimed at improving nuña bean cultivars, as well as for extending our knowledge of the genetic determinants and genotype x environment interaction involved in the popping ability traits of this bean crop.

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New microsatellite markers developed from an enriched microsatellite common bean library

Cited by 12 publications

References 14 publications

Dissection of Resistance Genes to Pseudomonas syringae pv. phaseolicola in UI3 Common Bean Cultivar

Dissection of Resistance Genes to Pseudomonas syringae pv. phaseolicola in UI3 Common Bean Cultivar

Major Contribution of Flowering Time and Vegetative Growth to Plant Production in Common Bean As Deduced from a Comparative Genetic Mapping

Marker-based linkage map of Andean common bean (Phaseolus vulgarisL.) and mapping of QTLs underlying popping ability traits

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