New nanoparticles for topical ocular delivery of erythropoietin

Silva, Beatriz; Marto, Joana; Bráz, Berta São; Delgado, Esmeralda; Almeida, António J.; Gonçalves, Lídia

doi:10.1016/j.ijpharm.2020.119020

Cited by 48 publications

(57 citation statements)

References 68 publications

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“…The study was performed with the cornea used in the ex-vivo permeation study. 26 The treated cornea removed from the diffusion cell after permeation study and placed into formalin solution (8% v/v). The cornea was washed with alcohol to remove water and fixed with paraffin wax for making the solid block.…”

Section: Histopathological Examinationmentioning

confidence: 99%

Clarithromycin-Loaded Ocular Chitosan Nanoparticle: Formulation, Optimization, Characterization, Ocular Irritation, and Antimicrobial Activity

Bin-Jumah

Gilani

Jahangir³

et al. 2020

IJN

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The topically administered drugs through conventional delivery systems have low bioavailability. Henceforth, the present study was designed to prepare and optimize clarithromycin (CTM)-loaded chitosan nanoparticles (CHNPs) to demonstrate the efficacy against microorganisms. Methods: Clarithromycin-loaded chitosan nanoparticles (CTM-CHNPs) were prepared by ionotropic gelation method. The formulation was optimized by box-Behnken design using the formulation variables like CH (A), STPP concentration (B), and stirring speed (C). Their effects were evaluated on the independent variables like particle size (Y 1) and entrapment efficiency (Y 2). Further, CTM-CHNPs were evaluated for physicochemical parameters, invitro drug release, ex-vivo permeation, bioadhesive study, corneal hydration, histopathology, HET-CAM, and antibacterial study. Results: The optimized formulation (CTM-CHNPopt) showed the low particle size (152±5 nm), which is desirable for ocular delivery. It also showed high encapsulation (70.05%), zeta potential (+35.2 mV), and was found in a spherical shape. The drug release study revealed a sustained drug release profile (82.98±3.5% in 12 hours) with Korsmeyer peppas kinetic (R 2 =0.996) release model. It showed a 2.7-fold higher corneal permeation than CTMsolution. CHNPs did not exhibit any sign of damage to excised goat cornea, which is confirmed by hydration, histopathology, and HET-CAM test. It exhibited significant (P<0.05) higher antibacterial susceptibility than CTM-solution. Conclusion: The finding of the study concluded that CTM-CHNPs can be used for effective management of bacterial conjunctivitis by increasing the precorneal residence time.

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Section: Histopathological Examinationmentioning

confidence: 99%

Clarithromycin-Loaded Ocular Chitosan Nanoparticle: Formulation, Optimization, Characterization, Ocular Irritation, and Antimicrobial Activity

Bin-Jumah

Gilani

Jahangir³

et al. 2020

IJN

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“…The antimicrobial properties of ceftazidime were retained after its encapsulation into NPs, and the produced nanoparticulate system exhibited optimal characteristics for the local ocular antibiotic delivery. Silva et al further encapsulated erythropoietin in CS/HA NPs [177]. Hyaluronic acids of different molecular weights and sources were investigated.…”

Section: Ionotropic Gelation With Other Polymersmentioning

confidence: 99%

Chitosan and its Derivatives for Ocular Delivery Formulations: Recent Advances and Developments

et al. 2020

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Chitosan (CS) is a hemi-synthetic cationic linear polysaccharide produced by the deacetylation of chitin. CS is non-toxic, highly biocompatible, and biodegradable, and it has a low immunogenicity. Additionally, CS has inherent antibacterial properties and a mucoadhesive character and can disrupt epithelial tight junctions, thus acting as a permeability enhancer. As such, CS and its derivatives are well-suited for the challenging field of ocular drug delivery. In the present review article, we will discuss the properties of CS that contribute to its successful application in ocular delivery before reviewing the latest advances in the use of CS for the development of novel ophthalmic delivery systems. Colloidal nanocarriers (nanoparticles, micelles, liposomes) will be presented, followed by CS gels and lenses and ocular inserts. Finally, instances of CS coatings, aiming at conferring mucoadhesiveness to other matrixes, will be presented.

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“…The histopathological study was performed on the treated cornea with the tested samples in the permeation study. 10 After completion of the permeation study, the cornea was removed and stored into a formalin solution (8% v/v). The cornea was dehydrated with alcohol and the solid block was prepared with paraffin wax.…”

Section: Histopathological Examinationmentioning

confidence: 99%

“…9 Erythropoietin entrapped topical chitosan nanoparticles were developed and depicted strong mucoadhesion over procine cornea and conjunctiva. 10 Yu and its associates developed dexamethasone chitosan nanoparticles for enhancement of bioavailability through ocular delivery. It exhibited prolong in vitro release and precorneal residence time than an aqueous solution.…”

Section: Introductionmentioning

confidence: 99%

Stimulus Responsive Ocular Gentamycin-Ferrying Chitosan Nanoparticles Hydrogel: Formulation Optimization, Ocular Safety and Antibacterial Assessment

Alruwaili¹,

Zafar²,

Imam³

et al. 2020

IJN

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Purpose: The present study was designed to study the gentamycin (GTM)-loaded stimulusresponsive chitosan nanoparticles to treat bacterial conjunctivitis. Methods: GTM-loaded chitosan nanoparticles (GTM-CHNPs) were prepared by ionotropic gelation method and further optimized by 3-factor and 3-level Box-Behnken design. Chitosan (A), sodium tripolyphosphate (B), and stirring speed (C) were selected as independent variables. Their effects were observed on particle size (PS as Y1), entrapment efficiency (EE as Y2), and loading capacity (LC as Y3). Results: The optimized formulation showed the particle size, entrapment efficiency, and loading capacity of 135.2±3.24 nm, 60.18±1.65%, and 34.19±1.17%, respectively. The optimized gentamycin-loaded chitosan nanoparticle (GTM-CHNPopt) was further converted to the stimulus-responsive sol-gel system (using pH-sensitive carbopol 974P). GTM-CHNPopt sol-gel (NSG5) exhibited good gelling strength and sustained release (58.99 ±1.28% in 12h). The corneal hydration and histopathology of excised goat cornea revealed safe to the cornea. It also exhibited significant (p<0.05) higher ZOI than the marketed eye drop. Conclusion: The finding suggests that GTM-CHNP-based sol-gel is suitable for ocular delivery to enhance the corneal contact time and improved patient compliance.

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New nanoparticles for topical ocular delivery of erythropoietin

Cited by 48 publications

References 68 publications

<p>Clarithromycin-Loaded Ocular Chitosan Nanoparticle: Formulation, Optimization, Characterization, Ocular Irritation, and Antimicrobial Activity</p>

<p>Clarithromycin-Loaded Ocular Chitosan Nanoparticle: Formulation, Optimization, Characterization, Ocular Irritation, and Antimicrobial Activity</p>

Chitosan and its Derivatives for Ocular Delivery Formulations: Recent Advances and Developments

<p>Stimulus Responsive Ocular Gentamycin-Ferrying Chitosan Nanoparticles Hydrogel: Formulation Optimization, Ocular Safety and Antibacterial Assessment</p>

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