New nanostructured support for carrier-mediated electromembrane extraction of high polar compounds

Román-Hidalgo, Cristina; Martín-Valero, María Jesús; Fernández‐Torres, Rut; Mochón, M. Callejón; Bello‐López, Miguel Ángel

doi:10.1016/j.talanta.2016.10.022

Cited by 46 publications

(10 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Traces of these metabolites could however be observed when employing more manual, centrifugation-based sample preparation ( Figure S-4 ). A key reason is a weakness of EME, that highly polar compounds have low recovery; this can in many cases be fine-tuned 53,55 .…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Electromembrane extraction and mass spectrometry for liver organoid drug metabolism studies

Skottvoll

Hansen

Harrison

et al. 2020

Preprint

View full text Add to dashboard Cite

33Liver organoids (miniature, organ-like biomaterials derived from e.g. a patient´s stem 34 cells) are emerging tools for precision drug development and toxicity screening. We 35 demonstrate that electromembrane extraction (EME) is suited for collecting organoid-36 derived drug metabolites prior to mass spectrometry (MS)-based measurements. 37 EME, which is essentially electrophoresis across an oil membrane, allowed drugs 38 and drug metabolites to be separated from medium components (albumin, etc.) that 39 could interfere with subsequent measurements. Multi-well EME (100 µL solutions) 40 allowed for simple and repeatable monitoring of heroin metabolism kinetics. Organoid 41 EME extracts were compatible with ultrahigh-pressure liquid chromatography 42 (UHPLC) and capillary electrophoresis (CE), used to separate the analytes prior to 43 detection. These initial efforts show that organoids are well-matched with various 44 electrophoresis/chromatography techniques and MS measurements. 45 46 47

show abstract

Section: Resultsmentioning

confidence: 99%

“…this can in many cases be fine-tuned 53,55 . To complement the observations of the liver organoids enzymatic heroin metabolizing properties, a case study using MS-based untargeted proteomics was undertaken.…”

Section: Parallel Electromembrane Extraction Of Liver Organoid Heroinmentioning

confidence: 99%

Electromembrane extraction and mass spectrometry for liver organoid drug metabolism studies

Skottvoll

Hansen

Harrison

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Thus, there have been only a few investigations into alternative materials. Recently, however, a new support was proposed based on 100 µm thickness sheets of acrylic nanofiber membrane manufactured by electrospinning [43] . An SLM comprising Aliquat 336 dissolved in octanol was immobilized in this support, and used to extract the highly polar acidic drugs such as nicotinic acid, amoxicillin, hippuric acid, and salicylic acid.…”

Section: Recent Trendsmentioning

confidence: 99%

Electromembrane extraction–Recent trends and where to go

Pedersen‐Bjergaard

Huang

Gjelstad

2017

Journal of Pharmaceutical Analysis

View full text Add to dashboard Cite

Electromembrane extraction (EME) is an analytical microextraction technique, where charged analytes (such as drug substances) are extracted from an aqueous sample (such as a biological fluid), through a supported liquid membrane (SLM) comprising a water immiscible organic solvent, and into an aqueous acceptor solution. The driving force for the extraction is an electrical potential (dc) applied across the SLM. In this paper, EME is reviewed. First, the principle for EME is explained with focus on extraction of cationic and anionic analytes, and typical performance data are presented. Second, papers published in 2016 are reviewed and discussed with focus on (a) new SLMs, (b) new support materials for the SLM, (c) new sample additives improving extraction, (d) new technical configurations, (e) improved theoretical understanding, and (f) pharmaceutical new applications. Finally, important future research objectives and directions are defined for further development of EME, with the aim of establishing EME in the toolbox of future analytical laboratories.

show abstract

“…In most papers related to EME of acidic substances, 1‐heptanol or 1‐octanol were found to be the most efficient SLMs . To our knowledge, only one single paper is available in the literature describing on‐chip EME of acidic drugs , where simultaneous extraction of acidic and basic drugs was proposed, achieving the best extraction efficiency with 1‐octanol as SLM for the acidic analytes.…”

Section: Introductionmentioning

confidence: 99%

On‐chip electromembrane extraction of acidic drugs

et al. 2019

View full text Add to dashboard Cite

In the present work, a new supported liquid membrane (SLM) has been developed for on‐chip electromembrane extraction of acidic drugs combined with HPLC or CE, providing significantly higher stability than those reported up to date. The target analytes are five widely used non‐steroidal anti‐inflammatory drugs (NSAIDs): ibuprofen (IBU), diclofenac (DIC), naproxen (NAX), ketoprofen (KTP) and salicylic acid (SAL). Two different microchip devices were used, both consisted basically of two poly(methyl methacrylate) (PMMA) plates with individual channels for acceptor and sample solutions, respectively, and a 25 µm thick porous polypropylene membrane impregnated with the organic solvent in between. The SLM consisting of a mixture of 1‐undecanol and 2‐nitrophenyl octyl ether (NPOE) in a ratio 1:3 was found to be the most suitable liquid membrane for the extraction of these acidic drugs under dynamic conditions. It showed a long‐term stability of at least 8 hours, a low system current around 20 µA, and recoveries over 94% for the target analytes. NPOE was included in the SLM to significantly decrease the extraction current compared to pure 1‐undecanol, while the extraction properties was almost unaffected. Moreover, it has been successfully applied to the determination of the target analytes in human urine samples, providing high extraction efficiency.

show abstract

New nanostructured support for carrier-mediated electromembrane extraction of high polar compounds

Cited by 46 publications

References 35 publications

Electromembrane extraction and mass spectrometry for liver organoid drug metabolism studies

Electromembrane extraction and mass spectrometry for liver organoid drug metabolism studies

Electromembrane extraction–Recent trends and where to go

On‐chip electromembrane extraction of acidic drugs

Contact Info

Product

Resources

About